Construction of a Genetic Sexing Strain for Aedes albopictus: a promising tool for the development of sterilizing insect control strategies targeting the tiger mosquito

Abstract

A good production rate of TiCoq males makes this GSS suitable for any control method based on mass production of Ae. albopictus sterilizing males. Despite limitations resulting from affected egg hatch as well as the nature of the used insecticide, the construction of this GSS paves the way for industrial sex separation of Ae. albopictus.

Potential Impact of Integrating the Sterile Insect Technique into the Fight against Disease-Transmitting Mosquitoes

“…albimanus was produced in the 1970s for the SIT project in El Salvador but was lost after the trial was terminated (Dame et al 2009). Producing GSSs by classical genetics is lengthy and labour-intensive because it relies on mutagenizing and screening large numbers of individual families (due to the low probability of mutagenesis producing the desired linkage event) (Lebon et al 2018;Ndo et al 2018). In Aedes, species sex is determined by a male-determining factor located on a small, non-recombining M locus on chromosome 1 and not on a heteromorphic sex chromosome (Hall et al 2015), making it more unlikely and therefore more laborious to achieve translocation of the selectable marker to the sex-determining chromosome (Papathanos et al 2018).…”

Sex separation ofAedesspp. mosquitoes for sterile insect technique application: a review

“…aegypti in Thailand, Kittayapong et al (2018) demonstrated that with the adjustable glass plates, 99% of the males were obtained for sterilization and release in the field, with only 0.06 AE 0.10% of contamination by females. Lebon et al (2018) developed the first GSS for Ae. albopictus.…”