CONSTRUCTION AND ANALYSIS OF ANTENNAL cDNA LIBRARY FROM RICE STRIPED STEM BORER, Chilo suppressalis (WALKER) (LEPIDOPTERA: PYRALIDAE), AND EXPRESSION PROFILES OF PUTATIVE ODORANT‐BINDING PROTEIN AND CHEMOSENSORY PROTEIN GENES

Abstract

In this study, we constructed a high-quality cDNA library from the antennae of the Chilo suppressalis (Walker) (Lepidoptera: Pyralidae). A total of 1,235 colonies with inserts greater than 0.7 kb were sequenced and analyzed. Homology searching coupled with bioinformatics analysis identified 15 and 7 cDNA sequences, respectively, encoding putative odorant-binding proteins (OBPs) and chemosensory proteins (CSPs). A phylogenetic tree of CsupCSPs showed that each CsupCSP has orthologs in Manduca sexta and Bombyx mori with strong bootstrapping support. One CSP was either very specific or more related to the CSPs of another species than to conspecific CSP. The expression profiles of the OBPs and CSPs in different tissues were measured by real-time quantitative PCR. The results revealed that of the 11 OBP genes, the transcript levels of CsupOBP1, CsupOBP5, and CsupOBP7 were higher in both male and female antennae than those in other tissues. And CsupCSP7 was highly expressed in both male and female antennae. Based on these results, the possible physiological functions of CsupOBPs and CsupCSPs were discussed.

Identification and expression profile of odorant‐binding proteins in Halyomorpha halys (Hemiptera: Pentatomidae)

“…3). Seven putative OBPs had the less common basic isoelectric point (pI), which was also observed in other species by Zhu et al (2012), Farias et al (2015) and Gong et al (2015). As expected, all of the HhalOBPs had an amino terminal signal peptide, comprising the first 16 to 26 residues.…”

“…(), Farias et al . () and Gong et al (). As expected, all of the HhalOBPs had an amino terminal signal peptide, comprising the first 16 to 26 residues.…”

Identification and expression patterns of chemosensory proteins in the black-back prominent moth, Clostera restitura (Lepidoptera: Notodontidae)

“…Interestingly, most of CresCSPs, such as CresCSP1, CresCSP2, CresCSP4, CresCSP5 and CresC-SP8 were mainly expressed in 3 to 4-day-old adults and, therefore, might be associated with mating behavior, in particular the secretion of sex-pheromone by females of C. restitura and their location by males. Many studies revealed that CSPs had a close connection with insect mating and egg-laying (Gong et al, 2012(Gong et al, , 2015Ju et al, 2014). Ligand-binding assays of CSPs in Plutella xylostella and Sesamia inferens reported higher binding to non-volatile oviposition deterrents and pheromone components, which supported the above suggestion (Liu et al, 2010;Zhang et al, 2014).…”