Identification and expression profile of odorant‐binding proteins in Halyomorpha halys (Hemiptera: Pentatomidae)

Abstract

The brown marmorated stink bug, Halyomorpha halys, is a devastating invasive species in the USA. Similar to other insects, olfaction plays an important role in its survival and reproduction. As odorant-binding proteins (OBPs) are involved in the initial semiochemical recognition steps, we used RNA-Sequencing (RNA-Seq) to identify OBPs in its antennae, and studied their expression pattern in different body parts under semiochemical stimulation by either aggregation or alarm pheromone or food odorants. Thirty full-length putative HhalOBPs were identified, corresponding to 22 'classic' OBPs and eight 'Plus-C' OBPs. The similarity amongst them ranged from 4.95-70.92%, and with another 325 hemipteran OBPs similarity ranged from 1.94-91.51%, the highest levels being with other stink bug OBPs. Phylogenetic analysis confirmed the monophyly of seven groups of stink bug and other hemipteran OBPs. All 30 HhalOBPs were expressed and about 2/3 were expressed primarily in antennae. The expression of 21 HhalOBPs was higher in the antennae under alarm pheromone stimulus, indicating that multiple OBPs may be responding to this pheromone. Two were highest in antennae under aggregation pheromone stimulus. These findings should provide a basis for understanding the physiological functions of HhalOBPs and the chemosensory perception of this pest, which may help to uncover new control targets for behavioural interference.

Transcriptome sequencing of Tessaratoma papillosa antennae to identify and analyze expression patterns of putative olfaction genes

“…papillosa transcripts through GO assignment generated results similar to those reported previously for dipteran 29–31 , coleopteran 32–34 , hymenopteran 35, 36 , lepidopteran 37–40 and other hemipteran antennal transcriptomes 41 . Such comparison with other insect orders suggested a certain level of conservation among the molecular component in the peripheral olfactory system.…”

“…papillosa (33 OBPs) are similar to the number reported in the antennal transcriptome of three plant bugs, Lygus lineolaris (32 OBPs) 49 , H . halys (30 OBPs) 41 and Apolygus lucorum (38 OBPs) 50 but more than the number expressed in the antennae of other Heteropteras (Pentatomidae) Adelphocoris lineolatus (14 OBPs) 51 , and Adelphocoris suturalis (16 OBPs) 52 . This difference could be potentially attributed to their specialized ecology.…”

“…Recently, the alarm pheromone stimulus-induced effect resulted in up-regulating the expression levels of twenty-one H . halys OBPs in the antennae, suggesting that multiple OBPs may respond to this pheromone 41 . Addition of antenna-predominant candidates to the existing olfactory related gene pool could provide clues to future studies on the functional characterization of T .…”

Molecular identification and expression patterns of odorant binding protein and chemosensory protein genes inAthetis lepigone(Lepidoptera: Noctuidae)

“…Many studies show that the periphery process of insect olfaction requires a set of genes, including those that encode odorant binding proteins (OBPs), chemosensory proteins (CSPs), and chemosensory receptors (Elfekih et al, 2016; Glaser et al, 2015; Larter, Sun & Carlson, 2016; Li et al, 2015; Paula et al, 2016; Zhang et al, 2013). Generally, OBPs/CSPs located in the antennal sensillar lymph can recognize and bind external odorants that can then be transferred by OBPs/CSPs through the sensillar lymph to chemosensory receptors, odorant receptors (ORs) and ionotropic receptors (IRs).…”

Identification and expression analysis of chemosensory genes in the tea green leafhopper, Empoasca onukii Matsuda

“…With the development and improvement of the genome and transcriptome sequencing technique, a large number of OBP and CSP genes have been identified from diverse orders, including Hemiptera (Cui et al., ), Lepidoptera (Yao, Xu, Dong, Lu, & Chen, ), Diptera (Wang, Liu, & Wang, ; Wang, Li, et al., ), Hymenoptera (Zhang, Zhang, Su, Gao, & Guo, ), Orthoptera (Ban et al., ), Isoptera (Krieger & Ross, ) and Coleoptera (Antony et al., ). In the Hemiptera insects, numerous OBP and CSP genes have been identified from diverse families, including Miridae [such as Apolygus lucorum (Yuan et al., ), Adelphocoris lineolatus (Gu et al., ; Sun, Wang, Wang, et al., ), Adelphocoris suturalis (Cui et al., ) and Lygus lineolaris (Hull, Perera, & Snodgrass, )], Aphididae [such as Aphis gossypii (Gu, Wu, et al., ), Sitobion avenae (Xue et al., ), Myzus persicae (Xu et al., ) and Acyrthosiphon pisum (Zhou et al., )], Delphacidae [such as Nilaparvata lugens (Zhou, Sun, Ma, Chen, & Wang, ), Sogatella furcifera (Ming & Peng, ) and Laodelphax striatellus (He, Zhang, Li, Li, & Dong, )] Aleyrodidae (such as Bemisia tabaci ; Wang, Liu, et al., ; Wang, Li, et al., ), Pseudococcidae [such as Phenacoccus solenopsis (Nie et al., )], Pentatomidae [such as Halyomorpha halys (Paula et al., )] and Reduviidae [such as Rhodnius prolixus (Ribeiro et al., )]. Furthermore, the functions of some OBP and CSP genes in the olfactory perception process of insects have also been predicted and verified.…”