Constitutive Promoters Available for Transgene Expression Instead of CaMV 35S RNA Promoter : Arabidopsis Promoters of Tryptophan Synthase Protein β Subunit and Phytochrome B

The activity of a predicted promoter, PMC8, from Milk vetch dwarf virus was evaluated by comparing it with the cauliflower mosaic virus 35S RNA promoter (P35S) and PNCR, a promoter from Soybean chlorotic mottle virus. When the GUS fusion gene was introduced into tobacco, PMC8 showed a similar expression profile to P35S but with a more intense expression in proliferating tissues. The usefulness of PMC8 was confirmed by driving NPTII for selection of kanamycin-resistant tobacco plants with improved transformation efficiency. PMC8 was also effective in transgenic rice plants. Thus, PMC8 is useful as an alternative to P35S in both dicotyledonous and monocotyledonous plants, especially for gene expression in proliferating tissues.

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The promoter of Milk vetch dwarf virus component 8 confers effective gene expression in both dicot and monocot plants

Shirasawa-Seo

Sano

Nakamura

et al. 2005

Plant Cell Rep

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Efficiency of the alpha-hairpinin SmAMP-X gene promoter from Stellaria media plant depends on selection of transgenic approach

Ivanova,

Komakhin

2023

Transgenic Res

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Promoter of chrysanthemum actin confers high-level constitutive gene expression in Arabidopsis and chrysanthemum

Hong

Suh

Kwon

et al. 2016

Scientia Horticulturae

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Constitutive Promoters Available for Transgene Expression Instead of CaMV 35S RNA Promoter : Arabidopsis Promoters of Tryptophan Synthase Protein β Subunit and Phytochrome B

Cited by 5 publications

References 22 publications

The promoter of Milk vetch dwarf virus component 8 confers effective gene expression in both dicot and monocot plants

The promoter of Milk vetch dwarf virus component 8 confers effective gene expression in both dicot and monocot plants

Efficiency of the alpha-hairpinin SmAMP-X gene promoter from Stellaria media plant depends on selection of transgenic approach

Promoter of chrysanthemum actin confers high-level constitutive gene expression in Arabidopsis and chrysanthemum

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