Promoter of chrysanthemum actin confers high-level constitutive gene expression in Arabidopsis and chrysanthemum

Hong, Joon Ki; Suh, Eun Jung; Kwon, Soo-Jin; Lee, Seung Bum; Kim, Jin A.; Lee, Soo In; Lee, Yeon-Hee

doi:10.1016/j.scienta.2016.08.006

Cited by 11 publications

(5 citation statements)

References 42 publications

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“…While we were performing our analysis, a report on the activity of another chrysanthemum actin promoter (CmActin, -1372 to +60) was published by another research group (Hong et al 2016). We compared the CmActin and CmACT2 promoters in the genome of Chrysanthemum seticuspe (Hirakawa et al 2019), which is a wild relative of Chrysanthemum morifolium.…”

Section: Discussionmentioning

confidence: 99%

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Parsley ubiquitin promoter displays higher activity than the CaMV 35S promoter and the chrysanthemum actin 2 promoter for productive, constitutive, and durable expression of a transgene in Chrysanthemum morifolium

2019

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The chrysanthemum (Chrysanthemum morifolium) is one of the most popular ornamental plants in the world. Genetic transformation is a promising tool for improving traits, editing genomes, and studying plant physiology. Promoters are vital components for efficient transformation, determining the level, location, and timing of transgene expression. The cauliflower mosaic virus (CaMV) 35S promoter is most frequently used in dicotyledonous plants but is less efficient in chrysanthemums than in tobacco or torenia plants. Previously, we used the parsley ubiquitin (PcUbi) promoter in chrysanthemums for the first time and analyzed its activity in transgenic calli. To expand the variety of constitutive promoters in chrysanthemums, we cloned the upstream region of the actin 2 (CmACT2) gene and compared its promoter activity with the 35S and PcUbi promoters in several organs, as well as its durability for long-term cultivation. The CmACT2 promoter has higher activity than the 35S promoter in calli but is less durable. The PcUbi promoter has the highest activity not only in calli but also in leaves, ray florets, and disk florets, and retains its activity after long-term cultivation. In conclusion, we have provided useful information and an additional type of promoter available for transgene expression in chrysanthemums.

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Section: Discussionmentioning

confidence: 99%

“…Therefore, we think that the origin of these two actin promoters is different. Using selected transformants, the CmActin promoter showed higher activity than the 35S promoter (Hong et al 2016). However, promoter activity also differed substantially among individual transformants (Figs.…”

Section: Author Contribution Statementmentioning

confidence: 93%

Parsley ubiquitin promoter displays higher activity than the CaMV 35S promoter and the chrysanthemum actin 2 promoter for productive, constitutive, and durable expression of a transgene in Chrysanthemum morifolium

2019

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“…Constitutive promoters such as CaMV35S were extensively used to drive expression of heterologous genes. However, they can cause additional metabolic burden or toxic effects, ultimately leading to morphological and physiological dysfunction [40,41]. Compared with constitutive promoters, the tissue-specific and induced promoters can fine-tune gene expression at different developmental stages and abiotic stresses.…”

Section: Rcfah12 Promoter Can Be Used For Genetic Engineeringmentioning

confidence: 99%

Identification and Functional Characterization of the RcFAH12 Promoter from Castor Bean in Arabidopsis thaliana

Wang

et al. 2022

Separations

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Castor (Ricinus communis L.) seed oil is the commercial source of ricinoleate, a valuable raw material used in many industries. Oleoyl-12-hydroxylase (RcFAH12) is a key enzyme in the biosynthesis of ricinoleate, accumulating nearly 90% of the triacylglycerol in castor seeds. Little is known about the transcriptional regulation of RcFAH12. We used rapid amplification of cDNA 5′ ends (5′RACE) to locate the transcription start site (TSS) of RcFAH12, and the sequence of a 2605 bp region, −2506~+99, surrounding the TSS was cloned. We then investigated these regions to promote β-glucuronidase (GUS) expression in transgenic Arabidopsis by the progressive 5′ and 3′ deletions strategies. The GUS staining showed that the GUS accumulation varied in tissues under the control of different deleted fragments of RcFAH12. In addition, the GUS expression driven by the RcFAH12 promoter markedly accumulated in transgenic seeds, which indicated that RcFAH12 might play an important role in the biosynthesis of ricinoleic acid. This study will lay a potential foundation for developing a tissue-specific promoter in oil-seed crops.

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“…The promoter of beta-actin gene has been isolated from and characterized in a wide variety of species such as human ( Gunning et al, 1987 ), chicken ( Kosuga et al, 2000 ), amphioxus ( Feng et al, 2014 ), teleosts ( Barman et al, 2015 ; Hwang et al, 2003 ; Kong et al, 2014 ; Liu et al, 1991 ; Noh et al, 2003 ) and shrimps ( Shi et al, 2015 ; Shi et al, 2016 ). Because the beta-actin gene promoter confers a high level of constitutive transcriptional expression, it is widely used for transgenesis in both plants and animals ( Cho et al, 2011 ; Hong et al, 2016 ; Kosuga et al, 2000 ). Although the cDNA of the beta-actin gene has been cloned from giant freshwater prawn ( Zhu et al, 2005 ), its promoter remains to be characterized.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of the giant freshwater prawn (Macrobrachium rosenbergii)beta-actingene promoter

Yan

Gao

Cui

et al. 2018

PeerJ

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Constitutive promoters are important tools for gene function studies and transgenesis. The Beta-actin (actb1) gene promoter has been isolated from many species but remains to be cloned from the giant freshwater prawn (Macrobrachium rosenbergii). In this study, we cloned and characterized the Mractb1 gene promoter. Two alternative promoters were identified for the Mractb1 gene, which direct the generation of two transcripts with different 5′ untranslated regions. Three CpG islands were predicted in the upstream sequence, which are intimately related to transcription initiation and promoter activity. In addition to the CCAAT-box and the CArG-box, molecular dissection of the flanking sequence revealed the existence of one negative and two positive elements in the upstream region and the first intron. Finally, the Mractb1 promoter demonstrated comparative activity to the carp (Cyprinus carpio) actb1 promoter. Our investigations provide a valuable genetic tool for gene function studies and shed light on the regulation of the Mractb1 gene.

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Promoter of chrysanthemum actin confers high-level constitutive gene expression in Arabidopsis and chrysanthemum

Cited by 11 publications

References 42 publications

Parsley <i>ubiquitin</i> promoter displays higher activity than the CaMV 35S promoter and the chrysanthemum <i>actin 2</i> promoter for productive, constitutive, and durable expression of a transgene in <i>Chrysanthemum morifolium</i>

Parsley <i>ubiquitin</i> promoter displays higher activity than the CaMV 35S promoter and the chrysanthemum <i>actin 2</i> promoter for productive, constitutive, and durable expression of a transgene in <i>Chrysanthemum morifolium</i>

Identification and Functional Characterization of the RcFAH12 Promoter from Castor Bean in Arabidopsis thaliana

Molecular characterization of the giant freshwater prawn (Macrobrachium rosenbergii)beta-actingene promoter

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