The promoter of Milk vetch dwarf virus component 8 confers effective gene expression in both dicot and monocot plants

Shirasawa-Seo, Naomi; Sano, Yoshitaka; Nakamura, Shigeo; Murakami, Taka; Gotoh, Yoko; Naito, Yumi; Hsia, Chi Ni; Seo, Shigemi; Mitsuhara, Ichiro; Kosugi, Shunichi; Ohashi, Yuko

doi:10.1007/s00299-005-0917-0

Cited by 14 publications

(7 citation statements)

References 28 publications

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“…Furthermore, the 35S promoter may also drive TfGLO - and TfDEF - SRDX insufficiently to repress stamen development. Activity of the 35S promoter was different in each organ and was low in some organs and tissues (Mitsuhara et al 1996; Shirasawa-Seo et al 2005). Utilization of their own promoters may provide a more accurate understanding of the functions of torenia class B genes in floral development, especially in stamens.…”

Section: Discussionmentioning

confidence: 99%

Functional divergence within class B MADS-box genes TfGLO and TfDEF in Torenia fournieri Lind

et al. 2010

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Homeotic class B genes GLOBOSA (GLO)/PISTILLATA (PI) and DEFICIENS (DEF)/APETALA3 (AP3) are involved in the development of petals and stamens in Arabidopsis. However, functions of these genes in the development of floral organs in torenia are less well known. Here, we demonstrate the unique floral phenotypes of transgenic torenia formed due to the modification of class B genes, TfGLO and TfDEF. TfGLO-overexpressing plants showed purple-stained sepals that accumulated anthocyanins in a manner similar to that of petals. TfGLO-suppressed plants showed serrated petals and TfDEF-suppressed plants showed partially decolorized petals. In TfGLO-overexpressing plants, cell shapes on the surfaces of sepals were altered to petal-like cell shapes. Furthermore, TfGLO- and TfDEF-suppressed plants partially had sepal-like cells on the surfaces of their petals. We isolated putative class B gene-regulated genes and examined their expression in transgenic plants. Three xyloglucan endo-1,4-beta-d-glucanase genes were up-regulated in TfGLO- and TfDEF-overexpressing plants and down-regulated in TfGLO- and TfDEF-suppressed plants. In addition, 10 anthocyanin biosynthesis-related genes, including anthocyanin synthase and chalcone isomerase, were up-regulated in TfGLO-overexpressing plants and down-regulated in TfGLO-suppressed plants. The expression patterns of these 10 genes in TfDEF transgenic plants were diverse and classified into several groups. HPLC analysis indicated that sepals of TfGLO-overexpressing plants accumulate the same type of anthocyanins and flavones as wild-type plants. The difference in phenotypes and expression patterns of the 10 anthocyanin biosynthesis-related genes between TfGLO and TfDEF transgenic plants indicated that TfGLO and TfDEF have partial functional divergence, while they basically work synergistically in torenia.Electronic supplementary materialThe online version of this article (doi:10.1007/s00438-010-0574-z) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Functional divergence within class B MADS-box genes TfGLO and TfDEF in Torenia fournieri Lind

et al. 2010

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“…Using this promoter, fairly high levels of accumulation of recombinant proteins have been achieved (Fiedler et al ., 1997; Gutierrez‐Ortega et al ., 2005). Other plant viral promoters have also been tested, including a cassava vein mosaic virus promoter (Verdaguer et al ., 1996), the C1 promoter of cotton leaf curl Multan virus (Xie et al ., 2003) and the promoter of component 8 of Milk vetch dwarf virus (Shirasawa‐Seo et al ., 2005). Most of the viral promoters tested also show activity in monocots (Shirasawa‐Seo et al ., 2005).…”

Section: Molecular Approaches To Boost Expressionmentioning

confidence: 99%

“…Other plant viral promoters have also been tested, including a cassava vein mosaic virus promoter (Verdaguer et al ., 1996), the C1 promoter of cotton leaf curl Multan virus (Xie et al ., 2003) and the promoter of component 8 of Milk vetch dwarf virus (Shirasawa‐Seo et al ., 2005). Most of the viral promoters tested also show activity in monocots (Shirasawa‐Seo et al ., 2005). Some plant viral promoters show tissue specificity (Mazithulela et al ., 2000).…”

Section: Molecular Approaches To Boost Expressionmentioning

confidence: 99%

Approaches to achieve high‐level heterologous protein production in plants

Streatfield

2006

Plant Biotechnology Journal

349

226

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SummaryPlants offer an alternative to microbial fermentation and animal cell cultures for the production of recombinant proteins. For protein pharmaceuticals, plant systems are inherently safer than native and even recombinant animal sources. In addition, post-translational modifications, such as glycosylation, which cannot be achieved with bacterial fermentation, can be accomplished using plants. The main advantage foreseen for plant systems is reduced production costs. Plants should have a particular advantage for proteins produced in bulk, such as industrial enzymes, for which product pricing is low. In addition, edible plant tissues are well suited to the expression of vaccine antigens and pharmaceuticals for oral delivery. Three approaches have been followed to express recombinant proteins in plants: expression from the plant nuclear genome; expression from the plastid genome; and expression from plant tissues carrying recombinant plant viral sequences. The most important factor in moving plant-produced heterologous proteins from developmental research to commercial products is to ensure competitive production costs, and the best way to achieve this is to boost expression.Thus, considerable research effort has been made to increase the amount of recombinant protein produced in plants. This research includes molecular technologies to increase replication, to boost transcription, to direct transcription in tissues suited for protein accumulation, to stabilize transcripts, to optimize translation, to target proteins to subcellular locations optimal for their accumulation, and to engineer proteins to stabilize them. Other methods include plant breeding to increase transgene copy number and to utilize germplasm suited to protein accumulation. Large-scale commercialization of plant-produced recombinant proteins will require a combination of these technologies.

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“…The observed variation in expression is probably due to a positional effect and a different number of integrated copies of the transgenes in transgenic plants (Hobbs et al, 1990(Hobbs et al, , 1993Peach & Velten, 1991). Nevertheless, PMC8 confers efficient gene expression and may serve as an alternative to P35S, which has been used widely for genetic manipulation (Shirasawa-Seo et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Characteristics of the promoters derived from the single-stranded DNA components of Milk vetch dwarf virus in transgenic tobacco

Shirasawa-Seo

Sano

Nakamura

et al. 2005

Journal of General Virology

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Predicted promoter regions of Milk vetch dwarf virus (MDV) components (C1-C11) were isolated and fused with a b-glucuronidase (GUS) reporter gene and the characteristics of the promoters were examined. In transgenic tobacco calli, promoters of MDV C4 (encoding a cell-cycle link protein), C5 and C7 (both encoding unknown proteins), C6 (encoding a nuclear-shuttle protein) and C8 (encoding a movement protein) generated a stronger level of GUS expression than the Cauliflower mosaic virus 35S RNA promoter (P35S). In leaves of transgenic tobacco plants, the promoters of C5 and C8 conferred a level of GUS activity comparable to that of P35S. Histochemical GUS analysis showed that the promoters of C4-C9, the latter encoding a capsid protein, were active in phloem and meristematic tissue. The promoter of C8 was also active in mesophyll and cortex cell types. A low level of activity was found for the promoters of C11, which encodes a master replication-initiator protein (Rep), and C1, C2, C3 and C10, which encode additional Reps, in both transgenic tobacco calli and plants.

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The promoter of Milk vetch dwarf virus component 8 confers effective gene expression in both dicot and monocot plants

Cited by 14 publications

References 28 publications

Functional divergence within class B MADS-box genes TfGLO and TfDEF in Torenia fournieri Lind

Functional divergence within class B MADS-box genes TfGLO and TfDEF in Torenia fournieri Lind

Approaches to achieve high‐level heterologous protein production in plants

Characteristics of the promoters derived from the single-stranded DNA components of Milk vetch dwarf virus in transgenic tobacco

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