Constitutive overexpression of cytochrome P450 monooxygenase genes contributes to chlorantraniliprole resistance in <i>Chilo suppressalis</i> (Walker)

Xu, Lixin; Jun, Zhao; Sun, Yuhan; Xu, Donghui; Xu, Guangchun; Xu, Xiang; Zhang, Yueliang; Huang, Shuxian; Han, Zhaojun; Gu, Zhennan

doi:10.1002/ps.5171

Cited by 42 publications

(41 citation statements)

References 28 publications

(43 reference statements)

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“…A previous resistance monitoring study showed that C. suppressalis in the Jiangxi, Hunan, Henan, and Zhejiang provinces of China developed moderate to high resistance to chlorantraniliprole (64.10- to 249.60-fold) in the field [1,2,8]. In the present study, we found that C. suppressalis could develop high-level resistance to chlorantraniliprole in the laboratory under selection pressure, which was in line with previous findings [8,9]. The results indicated that C. suppressalis populations from different geographical areas seem to have a remarkable ability to develop resistance to this insecticide under continuous pressure.…”

Section: Discussionsupporting

confidence: 92%

“…To confirm a functional role of the overexpressed UGT genes in chlorantraniliprole resistance, we performed gene-silencing RNAi assays. Such technology has previously been used to analyze gene functions in C. suppressalis and has shown potential for developing new pest control measures [9,16,29]. Our results showed that dsRNA injection significantly silenced the target genes.…”

Section: Discussionmentioning

confidence: 59%

“…Understanding resistance mechanisms is a prerequisite for establishing resistance management strategies. An important metabolic resistance mechanism of chlorantraniliprole mediated by phase I enzyme P450s in C. suppressalis has been reported [9]; however, the increase in UGT enzyme activities is another main mechanism conferring insecticide resistance. The application of inhibitors is an effective way to verify an enzyme’s function.…”

Section: Discussionmentioning

confidence: 99%

“…After collection, a susceptible strain YYS was established by maintaining one part of the population on rice seedlings in the laboratory for 17 generations without exposure to insecticide. The rest of the population was continuously selected in the laboratory by treating fourth instar larvae with LC 50 of chlorantraniliprole (using the diet surface overlay bioassay method [9]) for 17 generations to obtain a resistant strain of YYR. The C. suppressalis colony was reared on rice seedlings at 27 ± 1 °C with a humidity of 70–80% and 16-h light/8-h dark photoperiod in the laboratory.…”

Section: Methodsmentioning

confidence: 99%

“…Previous studies have found that three mutations ( G4910E , Y4667D , and I4758M ) in RyR might be involved in the chlorantraniliprole resistance in C. suppressalis [7,8]. Additionally, multiple overexpressed cytochrome P450-dependent monooxygenase genes ( CYP6CV5 , CYP9A68 , CYP321F3 , and CYP32412 ) potentially contributed to chlorantraniliprole resistance of C. suppressalis [9]. Enhancement of esterase activity might also confer C. suppressalis resistance to chlorantraniliprole [7,8].…”

Section: Introductionmentioning

confidence: 99%

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UDP-Glycosyltransferase Genes in the Striped Rice Stem Borer, Chilo suppressalis (Walker), and Their Contribution to Chlorantraniliprole Resistance

Jun

Sun

et al. 2019

IJMS

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Uridine diphosphate glycosyltransferases (UGTs) are multifunctional detoxification enzymes, which are involved in metabolizing various chemicals and contribute to the development of insecticide resistance. However, the possible roles of UGTs in chlorantraniliprole resistance in Chilo suppressalis have rarely been studied in detail. Based on genome data, 24 UGT genes in C. suppressalis belonging to 11 families were identified, which were designated by the UGT nomenclature committee. Synergism assay data suggested that UGTs are potentially involved in chlorantraniliprole resistance in C. suppressalis. CsUGT40AL1 and CsUGT33AG3 were significantly overexpressed in the chlorantraniliprole resistant strain (12.36- and 5.34-fold, respectively). The two UGTs were highly expressed in the larval Malpighian tubules, fat body, and midgut; however, expression was lowest in the head. Injection of individual dsRNAs reduced the expression of the two target genes (by 69.34% and 48.74%, respectively) and caused significant higher larval mortality (81.33% and 54.67%, respectively). Overexpression of CsUGT40AL1 and CsUGT33AG3 was potentially involved in chlorantraniliprole resistance in C. suppressalis, as confirmed by the RNAi assay. Our findings suggest that overexpression of UGTs may contribute to chlorantraniliprole resistance in C. suppressalis.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 59%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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UDP-Glycosyltransferase Genes in the Striped Rice Stem Borer, Chilo suppressalis (Walker), and Their Contribution to Chlorantraniliprole Resistance

Jun

Sun

et al. 2019

IJMS

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Identification and transcriptional response of ATP‐binding cassette transporters to chlorantraniliprole in the rice striped stem borer, Chilo suppressalis

Meng

Yang

et al. 2020

Pest Management Science

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BACKGROUND: As the largest transporter gene family in metazoans, ATP-binding cassette (ABC) transporters regulate the efflux of a broad spectrum of substrates from the cytoplasm to the outside of the cell. In arthropods, ABC transporters are involved in phase III of the detoxification process, and play important roles in the metabolism and transport of insecticides. RESULTS: We identified 54 ABC transporters from the genome and transcriptome of Chilo suppressalis, one of the most damaging pests of rice in China. The identified ABC transporters were classified into eight subfamilies (ABCA to ABCH) based on NCBI BLAST and phylogenetic analysis. Synergism studies showed that treatment with verapamil, a potent inhibitor of ABC transporters, resulted in significantly increased toxicity of chlorantraniliprole against C. suppressalis larvae. Among the 21 tested ABC genes, three ABC transporter genes including CsABCC8, CsABCG1C and CsABCH1 were significantly upregulated after chlorantraniliprole treatment. CONCLUSION: ABC transporters play important roles in the detoxification and transport of chlorantraniliprole in C. suppressalis. The results from our study provide valuable information on C. suppressalis ABC transporters, and are helpful in understanding the roles of ABC transporters in chlorantraniliprole resistance mechanisms in C. suppressalis and other insect pests.

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Adipokinetic hormone signaling regulates cytochrome P450‐mediated chlorantraniliprole sensitivity in Spodoptera frugiperda (Lepidoptera: Noctuidae)

Tan

Chen

et al. 2022

Pest Management Science

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BACKGROUND: Fall armyworm (FAW, Spodoptera frugiperda) is one of the most destructive and invasive pests worldwide and causes significant economic losses. Intensive and frequent use of insecticides has led to the development of resistance in FAW. Adipokinetic hormone (AKH) have been proven to be involved in insecticide resistance in insects. However, the molecular mechanism underlying chlorantraniliprole resistance mediated by AKH signaling in FAW remains unclear.RESULTS: The expression of SpfAKHR was highest in male adults and lowest in 1st instar larvae. SpfAKH was expressed the highest in eggs and the lowest in 6th instar larvae. AKH signaling was involved in the sensitivity of FAW to chlorantraniliprole through a toxicological bioassay, and the combination of chlorantraniliprole and bithionol (an inhibitor of key enzymes in the AKH pathway) significantly increased the mortality of FAW. Chlorantraniliprole significantly induced the expression of ten P450s, SpfAKH and SpfAKHR in FAW. RNA interference against SpfAKHR significantly decreased the P450 content, downregulated the expression of three P450 genes (SpfCYP6B50, SpfCYP321A9 and SpfCYP9A58) and inhibited the resistance of FAW to chlorantraniliprole. The topical application of AKH peptide significantly increased the P450 content, upregulated the expression of five P450 genes (SpfCYP321A9, SpfCY321A8, SpfCYP321A10, SpfCYP321A7 and SpfCYP6AB12), and enhanced the survival of FAW against chlorantraniliprole.CONCLUSIONS: AKH plays an important role in enhancing chlorantraniliprole resistance in FAW by exerting a positive influence on P450 gene expression and P450 content. These results provide valuable insights into insecticide resistance regulation and FAW control strategies.

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Constitutive overexpression of cytochrome P450 monooxygenase genes contributes to chlorantraniliprole resistance in Chilo suppressalis (Walker)

Abstract: Multiple overexpressed P450 genes were potentially associated with chlorantraniliprole resistance, as confirmed by the RNA interference (RNAi) assay. Our findings suggested that metabolic resistance to chlorantraniliprole might be mediated by P450s. © 2018 Society of Chemical Industry.

Cited by 42 publications

References 28 publications

UDP-Glycosyltransferase Genes in the Striped Rice Stem Borer, Chilo suppressalis (Walker), and Their Contribution to Chlorantraniliprole Resistance

UDP-Glycosyltransferase Genes in the Striped Rice Stem Borer, Chilo suppressalis (Walker), and Their Contribution to Chlorantraniliprole Resistance

Identification and transcriptional response of ATP‐binding cassette transporters to chlorantraniliprole in the rice striped stem borer, Chilo suppressalis

Adipokinetic hormone signaling regulates cytochrome P450‐mediated chlorantraniliprole sensitivity in Spodoptera frugiperda (Lepidoptera: Noctuidae)

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