Conservation of pBuM–2 satellite DNA sequences among geographically isolated Drosophila gouveai populations from Brazil

Franco, Fernando Faria; Kuhn, Gustavo C. S.; Sene, Fábio de Melo; Manfrin, Maura Helena

doi:10.1007/s10709-006-6248-4

Cited by 12 publications

(12 citation statements)

References 46 publications

(76 reference statements)

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“…Our analyses suggest that no specific nucleotide substitutions or indels were identified in MspI satDNA monomers, which could discriminate single populations or groups of geographically close populations at the scale of the Peninsula of Setúbal. Such a lack of intraspecific molecular structure in satDNA sequences has been observed in several Drosophila species (Kuhn and Sene, 2004;Franco et al, 2006), and was suggested to arise due to the combination of both past and current gene flow events among populations and independent homogenization mechanisms favoring monomers shared among the populations (Kuhn and Sene, 2004;Franco et al, 2010). Considering the very recent invasion of Portugal by B. xylophilus (Mota et al, 1999), and the limited dispersion capacity of the nematode, it is very unlikely that a significant amount of gene flow has occurred within the investigated area.…”

Section: Discussionmentioning

confidence: 74%

Sequence variability of the MspI satellite DNA family of the pinewood nematode Bursaphelenchus xylophilus at different geographic scales

Vieira

Castagnone

Mallez

et al. 2014

Molecular Phylogenetics and Evolution

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a b s t r a c tTandemly repeated sequences known as satellite DNA (satDNA) generally exhibit complex evolutionary patterns of concerted evolution in which mutations are homogenized and fixed in a stochastic process of molecular drive. Here, the nucleotidic variability of the MspI satDNA family of the pinewood nematode Bursaphelenchus xylophilus is analyzed in order to understand the evolutionary dynamics of satDNA at the intraspecific level. A total of 425 MspI monomer units, either PCR-amplified from isolates of local (Peninsula of Setúbal, Portugal) or worldwide origin, or retrieved from the B. xylophilus genome sequence, were characterized and compared. Whatever their origin, sliding window analysis of sequence variability patterns among monomers revealed low, moderate and highly variant domains, indicating that variable levels of evolutionary constraint may act upon the entire monomers. The phylogenetic inference based on the different sets of MspI satDNA family for this species shows a broad polymorphism of the individual monomers, which were distributed into four main clusters. However, such clustering appeared independent from the geographic origin of the nematodes, and could not discriminate isolates or groups of geographically close isolates. Rather, the formation of different phylogenetic groups within this satDNA family suggests an a priori embodying of a set of diverging repeats from a common ancestor satDNA library, which have been differently amplified along the evolutionary pathway of this species. The present work improves knowledge on the evolutionary dynamics of satDNA at the intraspecific level, and provides new information on satDNA sequence variability among natural populations sampled at a local geographic scale.

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Section: Discussionmentioning

confidence: 74%

Sequence variability of the MspI satellite DNA family of the pinewood nematode Bursaphelenchus xylophilus at different geographic scales

Vieira

Castagnone

Mallez

et al. 2014

Molecular Phylogenetics and Evolution

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“…In contrast with the DBC-150 family, the pBuM subfamilies are slightly AT-rich (66% on average) N=number of analyzed repeats. Data from a Kuhn & Sene (2005); b Kuhn et al (2003) and c Franco et al (2006); d Kuhn & Sene (2004). and present lower levels of sequence variability, from 1.9% to 5.0%.…”

Section: Satellite Dna Characterizationmentioning

confidence: 90%

Low rates of homogenization of the DBC-150 satellite DNA family restricted to a single pair of microchromosomes in species from the Drosophila buzzatii cluster

et al. 2007

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A satellite DNA family, termed DBC-150, comprises slightly GC-rich repeat units of approximately 150 bp that were isolated (by DNA digestions or PCR) from the genome of all seven Drosophila species from the buzzatii cluster (repleta group). The presence of subrepeats suggests that part of the extant DBC-150 monomer originated by the duplication of small sequence motifs. The DBC-150 family is compared to the previously described pBuM satDNA family, an abundant component of the genome of five species of the cluster. The two families are different in several aspects, including primary structure, A + T content, intraspecific and interspecific variability and rates of homogenization (or nucleotide spread). The data indicate a lower rate of homogenization (and absence of complete concerted evolution) of the DBC-150 compared to the pBuM family. FISH on metaphase chromosomes revealed that the DBC-150 family is located exclusively in the microchromosomes. To our knowledge this is the first record of a complex Drosophila satDNA restricted to a single pair of microchromosomes. The observed low rates of homogenization of the DBC-150 family might be related to a presumed reduction or suppression of meiotic recombination in the microchromosomes.

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“…However, the pBuM-1 and pBuM-2 evolutionarily related satDNA subfamilies from the D. buzzatii species cluster (repleta group) showed different sequence variability, indicating a slower rate of evolution of the pBuM-2 subfamily (Kuhn and Sene, 2005). This subfamily even showed high sequence conservation among geographically isolated populations from D. gouveai, a species included in this group (De Franco et al, 2006).…”

Section: Changes In Sequence Variabilitymentioning

confidence: 98%

Satellite DNA in insects: a review

2008

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Conservation of pBuM–2 satellite DNA sequences among geographically isolated Drosophila gouveai populations from Brazil

Cited by 12 publications

References 46 publications

Sequence variability of the MspI satellite DNA family of the pinewood nematode Bursaphelenchus xylophilus at different geographic scales

Sequence variability of the MspI satellite DNA family of the pinewood nematode Bursaphelenchus xylophilus at different geographic scales

Low rates of homogenization of the DBC-150 satellite DNA family restricted to a single pair of microchromosomes in species from the Drosophila buzzatii cluster

Satellite DNA in insects: a review

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