Conservation of medicinal yam <i>in vitro</i>: Effect of ionic strength, sucrose, mannitol, ABA and low temperature

Sanghamitra, Priyadarsini; Samantaray, Sanghamitra; Bagchi, Torit Baran; Mandal, B. B.

doi:10.5958/0974-0112.2019.00110.5

Cited by 6 publications

(6 citation statements)

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“…However, despite the differentiated behavior between plants, one must consider that high values for shoot height observed in the present work are similar to other studies regarding in vitro conservation of germplasm. Sanghamitra et al (2019), while studying a medicinal yam, verified estimated values for this variable ranging from 4,14 cm for 18 °C to 6,88 cm for 25 °C height.…”

Section: Discussionmentioning

confidence: 64%

Effect of different temperatures for in vitro conservation of cassava (Manihot esculenta Crantz) germplasm

Santos¹,

Freitas²,

Souza³

et al. 2022

CLIUM

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The objective of the present work was to evaluate in vitro growth of cassava accessions under different temperatures to improve protocols for germplasm conservation. Acclimatized chambers were tested at 18 °C, 20 °C and 22 °C, and again at 22 °C in conservation room (control) in five accessions: Mandioca João Grande (BGM1325), Fécula Branca (BGM1632), BRS Kiriris (BGM1728), S/N (121) (BGM2004) and BRS Prata (BGM2019). After 90 days of distance, the following were evaluated: shoot height (cm), number of sprouts and microcuttings, number of green and senescent leaves, fresh and dry weight of shoots and roots (g). The experiment was performed under a completely randomized design in factorial scheme 5(accessions) x 4(temperatures), with 20 replicates. All data were submitted to the F test ANOVA and evaluated by the Tukey and Scott-Knott tests at 5% probability. The control temperature promoted lower mean values for plant shoot height, with emphasis for accessions BGM1325, BGM1632 and BGM2019. In a general manner accessions BGM1325, BGM1632 and BGM2019 showed lower SH, with the first and BGM2004 outstanding with the best results for lower NSL and higher NGL.

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Section: Discussionmentioning

confidence: 64%

Effect of different temperatures for in vitro conservation of cassava (Manihot esculenta Crantz) germplasm

Santos¹,

Freitas²,

Souza³

et al. 2022

CLIUM

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“…A similar conclusion was attained by Arrigoni-Blank et al (2014), who determined that the addition of ABA in the culture medium of sweet potato was efficient for some genotypes, while others showed detrimental effects. Sanghamitra et al (2019) obtained satisfactory growth reduction results in Dioscorea using higher dosages (10 mg L -1 ) of ABA.…”

Section: Resultsmentioning

confidence: 83%

“…In vitro conservation of germplasm using tissue culture techniques has emerged as an alternative to germplasm banks in the field, which are exposed to pests and pathogens, climatic conditions, vandalism, human failures, and errors in access identification, among others. In vitro conservation is convenient, as it is economically feasible and practical (Withers & Williams, 1998;Sanghamitra, Samantaray, Bagchi, & Mandal, 2019), which allows for the conservation of a large quantity of plants in a reduced space, enables the preservation of security copies of field accessions, and simplifies germplasm exchange and trade between institutions, regions, and countries.…”

Section: Introductionmentioning

confidence: 99%

Abscisic acid and in vitro conservation of Manihot wild species

Sá

Verde

Souza

et al. 2022

Acta Sci. Biol. Sci.

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Abscisic acid (ABA) is associated with bud dormancy, leaf abscission, and germplasm growth inhibition in in vitro conservation. We evaluated the effects of ABA in four wild Manihot accessions and one cassava accession (M. esculenta Crantz) to refine in vitro conservation methods for these species. The experiment was performed at the Laboratory for Tissue Culture from Embrapa, Cruz das Almas, Bahia State, Brazil. The statistical design was completely random in a 5 × 5 factorial scheme [(5 ABA dosages (0, 0.25, 0.50, 0.75, and 1 mg L-1) and 5 Manihot species (M. pseudoglaziovii, M. tristis, M. flabellifolia, M. chlorosticta, and M. esculenta)], with 15 replicates. Mini-cuttings of 1 cm were used, each inoculated in 10 mL of modified Murashige and Skoog medium, solidified with Phytagel® (2.4 g L-1) containing the respective ABA dosages. Tubes containing these mini-cuttings were placed in a germplasm conservation room with an irradiance of 30 µmol m-2 s-1, temperature of 22 ± 1°C, and photoperiod of 12 hours. Plant height (cm), the number of living and senescent leaves, shoots, and mini-cuttings (1 cm), and fresh and dry weights of shoots and roots (mg) were evaluated after 150 days. Growth reduction was prominent in M. pseudoglaziovii, M. tristis, and M. flabellifolia during the in vitro conservation period. In the present study, the addition of ABA did not promote the expected reduction in plant growth

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“…The woody plant medium (WPM) (Matos Alvim et al, 2020). Culture media for slow growth can use sucrose as a carbon source (Than, 2013;Sanghamitra et al, 2019;Menezes-Sá et al, 2019;El-Hawaz et al, 2019;Matos Alvim et al, 2020;Gomes et al, 2021). Another modification made to the culture medium is sorbitol and mannitol as carbon sources.…”

Section: Discussionmentioning

confidence: 99%

Germinación asimbiótica, conservación ex situ e in vitro regeneración de plantas de Catasetum integerrimum Hook

Puc¹,

Cool²

2022

polibotanica

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Podopterus mexicanus Humb. & Bonpl. Polygonaceae. "Rompe capa". Árboles o arbustos de 1.5-6.0 m de altura, ramas con espina terminal, braquiblastos, hojas fasciculadas, flores blancas a verdosas en fascículos, y frutos cubiertos por el perianto externo que forma alas delgadas y largamente decurrentes hacia el pedicelo. Crece en bosques tropicales caducifolios y bosques espinosos, sobre suelo rocoso negro derivado de rocas ígneas. En elevaciones de 550-760 m. Florece de abril a mayo y fructifica de junio a septiembre.

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Conservation of medicinal yam in vitro: Effect of ionic strength, sucrose, mannitol, ABA and low temperature

Cited by 6 publications

References 0 publications

Effect of different temperatures for in vitro conservation of cassava (Manihot esculenta Crantz) germplasm

Effect of different temperatures for in vitro conservation of cassava (Manihot esculenta Crantz) germplasm

Abscisic acid and in vitro conservation of Manihot wild species

Germinación asimbiótica, conservación ex situ e in vitro regeneración de plantas de Catasetum integerrimum Hook

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