Prior knowledge about the physiological behavior of seeds under storage conditions allows the use of appropriate techniques to maintain viability. The goal of the present study was to classify the behavior of Simarouba amara seeds regarding their physiological potential during storage. The seeds were obtained from mature fruits, collected from five parent plants located at the Joanes-Ipitanga Environmental Protection Area (EPA) (Simões Filho, Bahia) in January 2018. Two experiments were carried out in a completely randomized design. In the first one, seed desiccation tolerance was evaluated with seven moisture contents: 5%, 7.5%, 10%, 12.5%, 15%, 25% and 35.6% (initial moisture). A germination test was carried out to evaluate the seeds’ vigor. In the second experiment, the seeds were stored with moisture contents of 7.5%, 12.5% and 35.6% at temperatures of 8 °C, 15 °C and 20 °C) for two storage periods, two and four months, and samples were taken to determine moisture content and conduct the germination test. Germination tests were performed in BOD chambers at 30 °C and with 12-hour light photoperiod. Daily counts were performed for the number of germinated seeds. We measured germination percentage, mean germination time, uncertainty index, percentage of normal seedlings, and percentage of unviable seeds. Simarouba amara seeds tolerate desiccation up to 7.5% moisture content, but lost viability when stored under this condition. At four months of storage all seeds lost viability. Simarouba amara seeds are recalcitrant and lose viability before reaching two months of storage.
Avanços nos programas de melhoramento de citros podem ser alcançados com a obtenção de indivíduos haploides por meio do cultivo de anteras. As cidras, genótipo utilizado para esse estudo, são reconhecidas por seu valor terapêutico na medicina tradicional. Assim, o objetivo do presente trabalho foi de ajustar um protocolo para a regeneração de plantas de Citrus medica L., a partir de calos originados de anteras. A espécie Citrus medica L. foi estudada visando a regeneração de plantas a partir de calos induzidos por meio de anteras. Foram montados experimentos envolvendo calos
Defining the cardinal temperatures for germination in the scenario of climate change with prediction of an increased terrestrial temperature plays an important role in conserving plant resources. This study aimed to define the cardinal temperatures, the time of conducting germination tests, and to apply the thermal time model for seeds of Diospyros inconstans. Thermal screening was carried out, based on germination tests performed in BOD chambers, at constant temperatures of 10, 20, 25, 30 and 40 °C, under a photoperiod of 12 hours/light. The cardinal temperatures for germination of D. inconstans seeds are 26.7 °C (optimal temperature), 13.8 °C (minimum temperature) and 37.7 °C (maximum temperature). Germination tests for the species can be conducted at optimum temperature, lasting 26 days for radicle emission and 63 days for formation of normal seedlings.
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