Conservação da qualidade fisiológica de sementes de Inga uruguensis Hook. et Arn.

Bilia, Denise Augusta Camargo

doi:10.17801/0101-3122/rbs.v20n1p48-54

Cited by 22 publications

(24 citation statements)

References 5 publications

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“…During processing the seeds, the sarcotesta was removed, which caused removal of the inner layers of the seed coat as well, ending up with the naked embryo. Removal of the sarcotesta was an absolute prerequisite since it is rich in sugars (Figliolia and Kageyama, 1994) which results in excessive growth of microorganisms during storage (Zuniga, 1996) and during germination tests (Bilia et al, 1998).…”

Section: Methodsmentioning

confidence: 99%

Physiological and cytological aspects of Inga vera subsp. affinis embryos during storage

Faria

Davide

Silva

et al. 2006

Braz. J. Plant Physiol.

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The remarkably short storability of recalcitrant seeds has prevented their inclusion in programs of ex situ conservation. The causes of their desiccation sensitivity and rapid decline in viability during storage are not fully elucidated yet. In this study the highly recalcitrant, fully viable embryos of the tree species Inga vera subsp. affinis were stored under various conditions and analyzed physiologically and cytologically at intervals, in order to obtain more insights in their loss of viability during storage. Embryos stored fully hydrated at 5 o C fully lost viability in 18 d. Sealed storage of partially dehydrated embryos slightly prolonged their viability, with 95% germination being attained after 14 d of storage. However, after 30 d of storage, viability was completely lost. Storage of hydrated embryos in a solution of polyethylene glycol (PEG) at -1.7 MPa water potential was capable of maintaining high germinability until 30 d of storage. When added to the PEG solution, abscisic acid showed a strong temperature-dependent interaction, with a positive effect on the longevity of embryos stored at 20 o C and a negative effect on embryos stored at 5 o C. In any case, embryo viability could be maintained no longer than 50 d. Embryos from seeds collected from the same trees in the following year showed better storability and still attained 45% germination after 62 d of storage. Analysis of cellular alterations during storage and viability loss of the embryos showed disappearance of starch granules and various damages to the cells, such as cell wall folding and cytoplasm fragmentation. Key-words: abscisic acid, polyethylene glycol, recalcitrant seeds, seed conservationAspectos fisiológicos e citológicos de embriões de Inga vera subsp. affinis durante o armazenamento: A capacidade reduzida de armazenamento apresentada pelas sementes recalcitrantes tem impedido sua inclusão em programas de conservação ex situ. As causas da rápida queda da viabilidade durante o armazenamento ainda não estão totalmente elucidadas. Visando, portanto, a um melhor entendimento desse comportamento, embriões de sementes recalcitrantes de Inga vera subsp. affinis foram armazenados sob várias condições e avaliados periodicamente com relação a aspectos fisiológicos e citológicos. Embriões armazenados sem secagem, a 5 o C, perderam totalmente a viabilidade em 18 dias. Embriões parcialmente desidratados apresentaram 95% de germinação após 14 dias de armazenamento em sacos plásticos selados. Entretanto, após 30 dias de armazenamento, a viabilidade foi totalmente perdida. O armazenamento de embriões sem secagem em solução de polietileno glicol (PEG) a um potencial hídrico de -1,7 MPa foi capaz de manter uma alta capacidade de germinação até 30 dias de armazenamento. Quando adicionado à solução de PEG, o ácido abscísico mostrou uma forte interação com a temperatura de armazenamento, com um efeito positivo na longevidade dos embriões armazenados a 20 o C, porém um efeito negativo nos embriões armazenados a 5 o C. No entanto, em nenhuma condição...

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Section: Methodsmentioning

confidence: 99%

Physiological and cytological aspects of Inga vera subsp. affinis embryos during storage

Faria

Davide

Silva

et al. 2006

Braz. J. Plant Physiol.

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“…Partial drying of seeds with recalcitrant behavior can contribute to the preservation thereof. Bilia et al (1998) found that reducing the moisture content from 58% to 49% allowed for the preservation of physiological potential in the storage of Inga uruguensis seeds.…”

Section: Introductionmentioning

confidence: 99%

Fruit biometry and seed germination of Syagrus romanzoffiana (Cham.) Glassm

et al. 2013

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Fruit biometry and seed germination of Syagrus romanzoffi ana (Cham.) Glassm. ABSTRACTSyagrus romanzoffi ana (Cham.) Glassm. is a species native to Brazil that is of ecological and aesthetic importance. Th is study aims to describe the biometric characteristics of S. romanzoffi ana fruits and to assess the eff ects that treatments meant to overcome dormancy and partial drying have on seed germination. For biometric characterization, each repetition consisted of 50 fruits from eight clusters (total, 400 fruits). To evaluate methods for overcoming dormancy, seeds representative of populations were treated by perforation of the operculum; perforation of the operculum and immersion in water for 24 hours; cracking; exposure to fi re for three minutes; and non-removal of fi bers. Th ere was also a control group. In a second experiment, seeds were partially dried. We determined the proportional moisture content of newly harvested seeds (20.2%) and dried them down to moisture contents of 15.5% and 12.7%. All seeds were subjected to a germination test at 30°C. Th e fruits showed biometric variations, with an average of 21.7 mm in longitudinal diameter and 19.7 mm in polar diameter, and a fresh mass of 5.61 g, of which 59.29% was pulp. Th e highest germination rates and vigor to overcome dormancy occurred aft er the operculum was perforated and the seeds were soaked in water for 24 hours. Th e results of the partial drying treatments did not diff er suffi ciently to discourage dormancy in any signifi cant manner. Acta Botanica Brasilica 27(1): 147-154. 2013.

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“…Andréo et al (2006), por sua vez, controlando a mobilização de água entre a semente e o meio, obtiveram embriões com germinação superior a 80% após 90 dias de armazenamento, também a 10 °C, quando mantidos em substratos com soluções de PEG a -2,4 MPa. Bilia et al (1998), estudando os limites de tolerância à dessecação dessas sementes, conseguiram armazená-las, na mesma temperatura utilizada por aqueles autores, por até 60 dias quando o teor de água foi reduzido até 50%.…”

Section: Introductionunclassified

Sementes recalcitrantes: intolerantes a baixas temperaturas? Embriões recalcitrantes de Inga vera Willd. subsp. affinis (DC.) T. D. Penn. toleram temperatura sub-zero

Bonjovani

Barbedo

2008

Rev. bras. Bot.

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-(Recalcitrant seeds: intolerant to low temperatures? Recalcitrant embryos of Inga vera Willd. subsp. affinis (DC.) T. D. Penn., a tropical species, are tolerant to subzero temperature). Recalcitrant seeds are intolerant to both desiccation and low temperatures, the most important mechanisms of seed conservation, consequently their storage is difficult. Inga vera subsp. affinis produces the so-called recalcitrant seeds, since they do not survive to water content lower than 35%. In this work it is demonstrated that these seeds are tolerant to temperatures as low as -2 °C. Embryos of I. vera of different stages of maturity were stored at temperatures varying from 8 to -18 °C for until 45 days. Non-dried embryos and both dried to -4 MPa and -6 MPa were stored. Results showed that, despite all embryos died at -18 °C, some cold tolerance was obtained by mature embryos when stored at -2 °C, as long as they are dried to -4 MPa.

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Conservação da qualidade fisiológica de sementes de Inga uruguensis Hook. et Arn.

Cited by 22 publications

References 5 publications

Physiological and cytological aspects of Inga vera subsp. affinis embryos during storage

Physiological and cytological aspects of Inga vera subsp. affinis embryos during storage

Fruit biometry and seed germination of Syagrus romanzoffiana (Cham.) Glassm

Sementes recalcitrantes: intolerantes a baixas temperaturas? Embriões recalcitrantes de Inga vera Willd. subsp. affinis (DC.) T. D. Penn. toleram temperatura sub-zero

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