Conformation transition kinetics of regenerated Bombyx mori silk fibroin membrane monitored by time-resolved FTIR spectroscopy

Chen, Xin; Shao, Zhengzhong; Marinković, Nebojša; Miller, Lisa M.; Zhou, Ping; Chance, Mark R.

doi:10.1016/s0301-4622(00)00213-1

Cited by 279 publications

(218 citation statements)

References 41 publications

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“…32 When SF was immobilized on to PET surface through coating method, absorption bands at 1,623 cm -1 (assigned to amide I of SF β-sheet structure) and 1,520 cm -1 (assigned to the amide II of SF β-sheet structures) showed up (Figure 2), indicating the successful introduction of SF onto the PET surface. 33 A strong peak at 1,050 cm -1 in the FTIR spectrum of PET+SF+HAP was indicative of the ν3 vibrations of PO 4 3-. 34 Meanwhile, those typical absorption bands of PET and SF became weaker and even disappeared.…”

Section: Morphology Of Ligamentsmentioning

confidence: 98%

Enhancement of osseointegration of polyethylene terephthalate artificial ligament by coating of silk fibroin and depositing of hydroxyapatite

Jiang¹,

Wan

Yang

et al. 2014

IJN

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Background Application of artificial ligament in anterior cruciate ligament reconstruction is one of the research focuses of sports medicine but the biological tendon–bone healing still remains a problem. The preliminary study of hydroxyapatite (HAP) coating on the polyethylene terephthalate (PET) surface could effectively induce the osteoblast differentiation, but the tendon–bone healing was still not stable. As a green synthesis process, the biomimetic mineralization can simulate the natural bone growth in vitro and in vivo. Methods HAP crystals were grown under the guide of silk fibroin (SF) PET surface by biomimetic route. Several techniques including scanning electron microscopy, attenuated total reflectance Fourier transform infrared spectroscopy, X-ray diffraction, and energy-dispersive X-ray spectroscopy were utilized for proving the introduction of both SF and HAP. The viability and osseointegration of bone marrow stromal cells on the surface of three kinds of ligament, including PET group (non-coating group), PET+SF group (SF-coating group), and PET+SF+HAP group (combined HAP- and SF-coating group), were analyzed by CCK-8 assays and alkaline phosphatase (ALP) detection. Seventy-two mature male New Zealand rabbits were randomly divided into three groups. Among them, 36 rabbits were sacrificed for mechanical testing, and histological examination for the others. Results The SF and SF+HAP were successfully coated on the surface of PET fiber. The CCK-8 assay showed that the cell proliferation on PET+SF+HAP group was better than the other two groups from 24 to 120 hours. After 14 days of culture, the cells in the PET+SF+HAP group delivered higher levels of ALP than the other two groups. After 3 days of culture, the expression level of integrin β1 in the PET+SF+HAP group and PET+SF group were higher than in the PET group. The mean load to failure and the stiffness value of the PET+SF+HAP group were both higher than the other two groups. Hematoxylin and eosin staining showed that new bone tissue formation was only found in the PET+SF+HAP group 8 weeks postoperatively. Masson staining showed that in the PET+SF+HAP group 8 weeks postoperatively, the PET fibers were almost completely encircled by collagen. Histomorphometric analysis showed that the width of the graft–bone interface in the PET+SF+HAP group was narrower than that in the other two groups 4 and 8 weeks postoperatively. The mRNA level of BMP-7 in the PET+SF+HAP groups was significantly higher than those in the other two groups 4 and 8 weeks postoperatively. Conclusion The study showed that the combined SF and HAP coating by biomimetic route on the surface of PET artificial ligament could induce graft osseointegration in the bone tunnel, providing theoretical and experimental foundation for manufacturing novel artificial ligaments meeting the clinical needs.

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Section: Morphology Of Ligamentsmentioning

confidence: 98%

Enhancement of osseointegration of polyethylene terephthalate artificial ligament by coating of silk fibroin and depositing of hydroxyapatite

Jiang¹,

Wan

Yang

et al. 2014

IJN

Self Cite

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“…For the characteristic peaks of silk fibroin in FT-IR spectra, see [25][26][27][28] . In Figure 4(a), relatively strong characteristic peaks appear in curve (A) at 1631 cm −1 (amide I), 1521 cm −1 (amide II), 1240 cm −1 (amide III), 965 cm −1 (amide IV), and 700 cm −1 (amide V), which means that pure A. pernyi SF hydrogels contain large amounts of β-sheets.…”

Section: Ft-ir Spectroscopymentioning

confidence: 99%

Comparison of Gelation Time and Polyalcohol Effect on Hydrogels from Domestic and Wild Silk Fibroins

Zhao

Xiong

et al. 2012

Advances in Materials Science and Engineering

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Silk fibroin (SF) hydrogels were obtained from both domestic (Bombyx mori) and wild (Antheraea pernyi) silkworms from aqueous silk fibroin solutions at room temperature. The gelation time of the Antheraea pernyi (A. pernyi) SF solution was significantly shorter than that of the Bombyx mori (B. mori) SF solution. The secondary structures of the two kinds of hydrogels were also compared. In order to further reduce the gelation time, various amounts of polyethylene glycol (PEG) were blended with the silk fibroins of A. pernyi and B. mori. The gelation time of both A. pernyi SF and B. mori SF decreased with the increased amount of PEG. After freeze-drying, the hydrogels were characterized through X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), and Raman spectroscopy. Results showed that the addition of polyalcohol did not change the main secondary structure of the hydrogels. However, the addition of polyalcohol did reduce the gelation time and triggered additional formation of β-sheets.

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“…The cross-linking with tripolyphosphate was confirmed by FTIR spectra. The peak at 1644 cm −1 represents the cross-linking of tripolyphosphate molecule with the amino group of chitosan through C=N (42)(43)(44)(45)(46). Cross-linking of nanofiber with glutaraldehyde is associated with the absorption peak near 1090 cm .…”

Section: Resultsmentioning

confidence: 99%

Electrospinning of Cross-Linked Magnetic Chitosan Nanofibers for Protein Release

2015

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Abstract. A poly(vinylalcohol) (PVA) electrospun/magnetic/chitosan nanocomposite fibrous cross-linked network was fabricated using in situ cross-linking electrospinning technique and used for bovine serum albumin (BSA) loading and release applications. Sodium tripolyphosphate (TPP) and glutaraldehyde (GA) were used as cross-linkers which modified magnetic-Fe 3 O 4 chitosan as Fe 3 O 4/ CS/TPP and Fe 3 O 4/ CS/GA, respectively. BSA was used as a model protein drugs which was encapsulated to form Fe 3 O 4 /CS/TPP/BSA and Fe 3 O 4 /CS/GA/BSA nanoparticles. The composites were electrospun with PVA to form nanofibers. Nanofibers were characterized by field emission scanning electron microscopy (FESEM) and Fourier transform infrared spectroscopy (FTIR). The characterization results suggest that Fe 3 O 4 nanoparticles with average size of 45 nm were successfully bound on the surface of chitosan. The cross-linked nanofibers were found to contain uniformly dispersed Fe 3 O 4 nanoparticles. The size and morphology of the nanofibers network was controlled by varying the cross-linker type. FTIR data show that these two polymers have intermolecular interactions. The sample with TPP cross-linker showed an enhancement of the controlled release properties of BSA during 30-h experimental investigation.

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Conformation transition kinetics of regenerated Bombyx mori silk fibroin membrane monitored by time-resolved FTIR spectroscopy

Cited by 279 publications

References 41 publications

Enhancement of osseointegration of polyethylene terephthalate artificial ligament by coating of silk fibroin and depositing of hydroxyapatite

Enhancement of osseointegration of polyethylene terephthalate artificial ligament by coating of silk fibroin and depositing of hydroxyapatite

Comparison of Gelation Time and Polyalcohol Effect on Hydrogels from Domestic and Wild Silk Fibroins

Electrospinning of Cross-Linked Magnetic Chitosan Nanofibers for Protein Release

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Conformation transition kinetics of regenerated Bombyx mori silk fibroin membrane monitored by time-resolved FTIR spectroscopy

Cited by 279 publications

References 41 publications

Enhancement of osseointegration of polyethylene terephthalate artificial ligament by coating of&nbsp;silk&nbsp;fibroin and depositing of hydroxyapatite

Enhancement of osseointegration of polyethylene terephthalate artificial ligament by coating of&nbsp;silk&nbsp;fibroin and depositing of hydroxyapatite

Comparison of Gelation Time and Polyalcohol Effect on Hydrogels from Domestic and Wild Silk Fibroins

Electrospinning of Cross-Linked Magnetic Chitosan Nanofibers for Protein Release

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Enhancement of osseointegration of polyethylene terephthalate artificial ligament by coating of silk fibroin and depositing of hydroxyapatite

Enhancement of osseointegration of polyethylene terephthalate artificial ligament by coating of silk fibroin and depositing of hydroxyapatite