Confocal Laser Scanning Microscopy Is Appropriate to Detect Viability of Enterococcus faecalis in Infected Dentin

Ordinola‐Zapata, Ronald; Bramante, Clóvis Monteiro; Moraes, Ivaldo Gomes de; Bernardineli, Norberti; Gasparoto, Thaís Helena; Graeff, Márcia Sirlene Zardin; Campanelli, Ana Paula; Garcia, Roberto Brandão

doi:10.1016/j.joen.2008.07.001

Cited by 102 publications

(103 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…During the last few years, different staining methods have been introduced to demonstrate viable and killed bacteria in dentin. Zapata et al (11) showed metabolic activity and viability of the E. faecalis cells inside dentinal tubules by 2 kinds of staining approaches. Nagayoshi et al (13) visualized bacteria within the tubules in a bovine dentin infection model.…”

Section: Discussionmentioning

confidence: 99%

“…A dentin block model, which has been adopted for the evaluation of the antibacterial effect in dentin for more than 2 decades, allowed bacterial penetration into dentinal tubules for up to 500 mm from the main root canal (10). More recent studies have visualized bacteria in dentinal tubules by confocal laser scanning microscopy (CLSM), which has been reported to be an appropriate way not just to visualize bacteria but also to identify live and dead bacteria in the infected dentin (11)(12)(13). However, a challenge remains how to obtain an equal (comparable), heavy presence of bacteria in all dentin specimens, because it has been difficult to replicate the clinical infection in dentin by cultural methods in vitro (14).…”

mentioning

confidence: 99%

See 1 more Smart Citation

A New Noninvasive Model to Study the Effectiveness of Dentin Disinfection by Using Confocal Laser Scanning Microscopy

Wang

Shen

et al. 2011

Journal of Endodontics

218

252

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

A New Noninvasive Model to Study the Effectiveness of Dentin Disinfection by Using Confocal Laser Scanning Microscopy

Wang

Shen

et al. 2011

Journal of Endodontics

218

252

View full text Add to dashboard Cite

“…Haapasalo and Ørstavik (14) developed a dentin block model for the evaluation of the antibacterial effect in dentin for more than 2 decades ago. More recent studies have observed the presence of live and dead bacteria in the dentinal tubules by CLSM (9,15). However, creating comparable and strong enough dentin infections has been difficult using culturing methods, making it challenging to quantitatively measure bacterial viability after exposure to disinfecting agents.…”

Section: Discussionmentioning

confidence: 99%

“…Compared with some previous bacterial invasion models based on culturing methods (14)(15)(16), the new model allowed a standardized, dense, and deep penetration of bacteria into dentinal tubules using the power of centrifugation. Hence, the application of this new model was suggested to provide a more predictable platform than previously to measure the effectiveness of disinfecting agents in infected dentin.…”

mentioning

confidence: 99%

The Effect of Detergents on the Antibacterial Activity of Disinfecting Solutions in Dentin

Wang¹,

Shen²,

Ma³

et al. 2012

Journal of Endodontics

View full text Add to dashboard Cite

“…Although the sample of tubes for the autoclave group did not show bacterial growth in the stipulated period of 6 hours after the initial analysis of these samples, they were incubated for a period of 24 hours in order to assess possible changes in an extended period, which was observed in 40% of the tubes. This late contamination of the samples can be attributed to the presence of viable microorganisms in dentinal tubules or due to the presence of cracks on the tooth surface 26 .…”

Section: Discussionmentioning

confidence: 99%

Assessment of the effects of decontamination and storage methods on the structural integrity of human enamel

Freitas

Aznar

Silva

et al. 2016

Rev. odontol. UNESP

View full text Add to dashboard Cite

ResumoIntrodução: O armazenamento de dentes para utilização em pesquisas é uma questão controvérsia e não há consenso sobre o método de tratamento mais apropriado para esta finalidade. Objetivo: O objetivo deste estudo foi analisar a efetividade e a influência de diferentes métodos de descontaminação e armazenamento do esmalte dentário humano, a fim de manter sua integridade. Material e método: A amostra foi constituída por 124 molares distribuídos aleatoriamente em três grupos, de acordo com o método: controle -água destilada, soluções de timol a 0,1% e de azida de sódio a 0,02%. Os testes realizados foram fluorescência a laser, microdureza de superfície e análise de perfilometria (0, 15 e 30 dias) e teste microbiológico (7, 15 e 30 dias). Os dados foram analisados por meio dos testes ANOVA e Tukey (p<0,05). Resultado: No teste de fluorescência a laser, a solução de timol a 0,1% demonstrou ser mais viável na manutenção da integridade do órgão dentário, uma vez que este não apresentou variações significativas nos valores entre os períodos avaliados (p<0,05). A análise de microdureza de superfície evidenciou perda de estrutura dentária em todos os métodos, sendo que a azida de sódio promoveu menor perda dentária. Na análise de perfilometria observou-se perda de estrutura em todos os grupos, com maior perda no grupo azida de sódio. Nenhum dos métodos conseguiu inibir o crescimento bacteriano. Conclusão: Dentre os métodos de processamento analisados nenhum foi capaz de aliar a efetividade na descontaminação ao armazenamento com manutenção da integridade estrutural do esmalte dentário humano.Descritores: Esmalte dentário; infecção; dureza; fluorescência. Abstract Introduction:The storage of teeth for use in research is a controversial issue with no consensus on the most appropriate treatment method for this purpose. Objective: The aim of this study was analyze the effectiveness and the influence of different methods of decontamination and storage of human enamel samples, in order to maintain their integrity. Material and method: The sample consisted of 124 molars distributed randomly into three groups according to the method: control -distilled water, 0.1% thymol and 0.02% sodium azide. The tests performed were laser fluorescence, surface microhardness and profilometry analysis (0, 15 and 30 days) and Microbiological test (7, 15 and 30 days). Data were analyzed by the ANOVA and Tukey tests (P <0.05). Result: In the laser fluorescence test, thymol proved to be more feasible for maintaining the integrity of the dental organ, since it did not show significant variations in values among the analyzed periods (P> 0.05). The surface microhardness analysis showed loss of tooth structure in all methods, and sodium azide led to a lower level of tooth loss. Profilometry analysis showed loss of mass in all groups whereas sodium azide showed the greatest loss. None of the methods was able to inhibit bacterial growth. Conclusion: Among the processing methods analyzed none was able to combine effective decontamination and storage ...

show abstract

Confocal Laser Scanning Microscopy Is Appropriate to Detect Viability of Enterococcus faecalis in Infected Dentin

Cited by 102 publications

References 36 publications

A New Noninvasive Model to Study the Effectiveness of Dentin Disinfection by Using Confocal Laser Scanning Microscopy

A New Noninvasive Model to Study the Effectiveness of Dentin Disinfection by Using Confocal Laser Scanning Microscopy

The Effect of Detergents on the Antibacterial Activity of Disinfecting Solutions in Dentin

Assessment of the effects of decontamination and storage methods on the structural integrity of human enamel

Contact Info

Product

Resources

About