A New Noninvasive Model to Study the Effectiveness of Dentin Disinfection by Using Confocal Laser Scanning Microscopy

Ma, Jia; Wang, Zhejun; Shen, Ya; Haapasalo, Markus

doi:10.1016/j.joen.2011.06.018

Cited by 224 publications

(295 citation statements)

References 30 publications

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“…A new non-invasive dentin disinfection model was proposed by Ma et al, in 2011 in an in-vitro study to establish bacterial presence within the dentinal tubules [19]. In comparison to bacterial invasion models based on culturing techniques that cannot be replicated in the clinical setup [20], this new model provided penetration of bacteria deep into the dentinal tubules by centrifugation and has been implemented in our study to standardize the methodology.…”

Section: Resultsmentioning

confidence: 99%

“…The teeth were stored in 0.01% sodium hypochlorite solution before use in order to clear them off the soft tissue debris. Based on a previously mentioned protocol [19], a root dentin block, with a length of 4 mm was horizontally sectioned from each tooth at 1 mm below the cementoenamel junction by a 0.6-mm-thick precision diamond saw (Isomet 5000; Buehler Ltd, LakeBluff, IL) at 1000 rpm under water cooling. Each cylindrical dentin block was fractured by first making a thin groove in the middle of the specimen by using low speed handpiece with a small round bur (Tulsa Dentsply) and then fracturing the specimen with a blade and a hammer into 2 semi cylindrical halves.…”

Section: Specimen Preparationmentioning

confidence: 99%

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The Effect of Addition of an EPS Degrading Enzyme with and without Detergent to 2% Chlorhexidine on Disruption of Enterococcus faecalis Biofilm: A Confocal Laser Scanning Microscopic Study

Ganesh

Nagendrababu

John

et al. 2015

JCDR

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Section: Resultsmentioning

confidence: 99%

Section: Specimen Preparationmentioning

confidence: 99%

The Effect of Addition of an EPS Degrading Enzyme with and without Detergent to 2% Chlorhexidine on Disruption of Enterococcus faecalis Biofilm: A Confocal Laser Scanning Microscopic Study

Ganesh

Nagendrababu

John

et al. 2015

JCDR

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“…Confocal laser scanning microscopy has some advantages compared to scanning electron microscopy, including histological evaluation and other methodologies for assessing the adaptation of endodontic sealers (18)(19)(20). This technology may also be used in microbiological studies for quantifying bacteria within dentinal tubules (8).…”

Section: Discussionmentioning

confidence: 99%

Filling Effectiveness and Dentinal Penetration of Endodontic Sealers: A Stereo and Confocal Laser Scanning Microscopy Study

et al. 2015

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The aim of this study was to evaluate the filling effectiveness and dentinal penetration of the sealers AH Plus, Pulp Canal Sealer EWT, Sealapex and MTA Fillapex applied according to the vertical condensation technique using thermoplastic gutta-percha. Forty singlerooted teeth were selected. After chemical-mechanical preparation and root-canal filling, sections of the root (2, 4 and 6 mm from the apex) were obtained and analyzed by stereo microscopy and confocal laser scanning microscopy. Data were tabulated and statistically analyzed. With regard to the assessment of void spaces in the filling material at 2 mm from the apex, the sealers showed similar results, but at 4 and 6 mm from the apex, MTA Fillapex had inferior performance compared to AH Plus (at 4 mm), Pulp Canal Sealer EWT (at 4 and 6 mm) and Sealapex (at 6 mm) (p<0.05). With regard to the penetration into dentinal tubules at 2 mm from the apex, the sealers also showed similar results, but at 4 and 6 mm Pulp Canal Sealer EWT had an inferior performance compared to MTA Fillapex and AH Plus, respectively (p<0.05). All four sealers were found to be similar regarding adaptation of the filling material to the root canal walls, except the MTA Fillapex, which showed failures at 4 and 6 mm from the root apex. With regard to the ability to penetrate into the dentinal tubules, the sealers were found to be equivalent, except for the Pulp Canal Sealer EWT as it had poorer results at 4 and 6 mm compared to MTA Fillapex and AH Plus, respectively.

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“…Another recent concern in endodontic irrigation is a potentially carcinogenic precipitate between sodium hypochlorite and CHX. A recent study by Ma et al (2011) showed QMiX to be as effective as 6% sodium hypochlorite against E. faecalis in dentinal tubules.…”

Section: Qmixmentioning

confidence: 99%

Enterococcus faecalis, a nightmare to endodontist: A systematic review

John¹,

Kumar²,

Gopal³

et al. 2015

Afr. J. Microbiol. Res.

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The main goal in endodontics is the prevention and treatment of diseases of the dental pulp and periapical tissues and it can be best achieved if preventive measures and treatment procedures are based on a thorough and detailed understanding of the etiology and pathogenesis of endodontic diseases. There are some cases in which the treatment has followed the highest technical standards and yet failure results. Scientific evidence indicates microbial factors which play an important role. In most cases, failure of endodontic treatment is a result of microorganisms persisting in the apical portion of the root canal system, even in well-treated teeth. Enterococcus faecalis is recognized as a pathogen in posttreatment endodontic infections and probably the species that can best adapt to and tolerate the ecologically demanding conditions in the filled root canal. Enterococci are also implicated in infections of the root canal system, however, they make up a small proportion of the initial flora which is dominated by Gram negative species. In contrast, it has been reported that enterococci are frequently isolated from obturated root canals of teeth that exhibit chronic periapical pathology. Eradication of E. faecalis from the root canal remains a challenge, while chlorhexidine and combinations of disinfectants show some promise. A better understanding of the role of the virulence factors of E. faecalis in endodontic infections may help in the development of new strategies to prevent or eliminate the infection by this species, thereby improving treatment results in endodontics.

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A New Noninvasive Model to Study the Effectiveness of Dentin Disinfection by Using Confocal Laser Scanning Microscopy

Cited by 224 publications

References 30 publications

The Effect of Addition of an EPS Degrading Enzyme with and without Detergent to 2% Chlorhexidine on Disruption of Enterococcus faecalis Biofilm: A Confocal Laser Scanning Microscopic Study

The Effect of Addition of an EPS Degrading Enzyme with and without Detergent to 2% Chlorhexidine on Disruption of Enterococcus faecalis Biofilm: A Confocal Laser Scanning Microscopic Study

Filling Effectiveness and Dentinal Penetration of Endodontic Sealers: A Stereo and Confocal Laser Scanning Microscopy Study

Enterococcus faecalis, a nightmare to endodontist: A systematic review

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