Concurrent Synthesis of Zero- and One-Dimensional, Spherical, Rod-, Needle-, and Wire-Shaped CuO Nanoparticles byProteus mirabilis10B

Abstract

Natural environment is a wealthy source of bionanofactories that invested in green approaches as the fabrication of biomimetic nanomaterials. The current study points out the importance of microbial activity in metal bioremediation, green synthesis of NPs, and global biogeochemical cycles of bioactive metals as well. It describes for the first time the synchronous biosynthesis of zero-(intracellular) and one-dimensional (extracellular) copper oxide nanoparticles (CuO-NPs) via Proteus mirabilis 10B. This bionanofactory represents key location of reduction and stabilization, and its exopolysaccharide additionally provides nucleation and growth site for CuO-NPs. The as-synthesized CuO-NPs were characterized; UV-Vis spectroscopy revealed surface plasmon resonance at 275 and 430 nm for intracellular and extracellular CuO-NPs, respectively. XRD reflected crystalline, pure phase monoclinic structure CuO-NPs. EDX illustrated strong copper signal with atomic percentages 32.3% (intracellular) and 14% (extracellular) CuO-NPs. However, ζ-potential recorded −62.5 and −43.8 mV with PDI 0.207 and 0.313 for intracellular and extracellular CuO-NPs, respectively, confirming the colloidal stability and monodispersity. Moreover, TEM micrographs depicted quasi-spherical intracellularly sequestered CuO-NPs (10 nm). Unexpectedly, extracellular CuO-NPs exhibited rod-, needle-, and wire-shaped with 17-37.5 nm in width and 112-615 nm in length. The antagonistic activity of both types of CuO-NPs was evaluated against Gram-negative and Gram-positive bacteria (aerobic and anaerobic), biofilm, yeast, mold, and algae. The potent antagonistic efficacy of CuO-NPs was displayed which encourages its utilization in controlling microbial contamination. Finally, the promising metabolic activity of Proteus mirabilis 10B can be exploited in simultaneous and beneficial applications for human and the surrounding ecosystem.

Efficacy Assessment of Biosynthesized Copper Oxide Nanoparticles (CuO-NPs) on Stored Grain Insects and Their Impacts on Morphological and Physiological Traits of Wheat (Triticum aestivum L.) Plant

“…The EDX spectra contained mainly the elements Cu and O, with weight percentages of 62.96% and 22.93%, respectively. The presence of S may have been due to the conjugation of CuO-NPs with fungal biomolecules found in the biomass filtrate, such as amino acids (cysteine or methionine) and phospholipids [ 52 ]. In line with this, Hassan et al [ 47 ] reported that the main EDX spectra peaks for CuO-NPs synthesized by endophytic actinomycetes were Cu and O, with the presence of other minor peaks related to biomolecules in the actinobacterial filtrate, which conjugated with CuO-NPs.…”

<p>Green Synthesis, Characterization and Antimicrobial Activity of Copper Oxide Nanomaterial Derived from <em>Momordica charantia</em></p>

“…36 However, as reaction proceeds with time, the copper (I) oxide was formed and finally CuO nanoparticles formation occur by a series of following reactions (Figure 1). [35][36][37]…”

“…It is the particle diffusion behavior within any fluid and is measured by the fluctuations in light intensity that passes through a colloidal solution as a function of time. 37 Further, zeta potential is considered as a measure of charges on the surface of nanoparticles. It indicates the stability of colloidal dispersions.…”

Unveiling the role of novel biogenic functionalized CuFe hybrid nanocomposites in boosting anticancer, antimicrobial and biosorption activities

“…Such naturally motivated technology addresses the art of designing materials in nanostructure configuration (less than 100 nm, at least in one dimension) that exhibit unique features surpassing their bulk-counterparts 1 , 2 . Over the recent decade, the paradigm leapfrogging in nanotechnology has led to an improvement in the methods of synthesizing nanomaterials that included the employment of green chemistry approaches 3 , 4 .…”