Unveiling the role of novel biogenic functionalized CuFe hybrid nanocomposites in boosting anticancer, antimicrobial and biosorption activities

Abstract

The quest for eco-friendly and biocompatible nanoparticles (NPs) is an urgent issue in the agenda of the scientific community and applied technology, which compressing synthesis routes. For the first time, a simple route for the biosynthesis of functionalized CuFe-hybrid nanocomposites (FCFNCs) was achieved using Streptomyces cyaneofuscatus through a simultaneous bioreduction strategy of Cu and Fe salts. The suitability of FCFNCs was evaluated medically and environmentally as an anticancer agent, antimicrobial agent and dye bio-sorbent. The physicochemical characteristics of FCFNCs using XRD, EDX, elemental mapping, FTIR, UV–Vis., TEM and ζ-potential confirmed the formation of spheres agglomerated into chains (37 ± 2.2 nm), self-functionalized nanocomposite by proteinaceous moieties with considerable stability (− 26.2 mV). As an anticancer agent, FCFNCs displayed the highest apoptotic impact (> 77.7%) on Caco-2, HepG-2, MCF-7 and PC-3 cancer cells at IC50 ≤ 17.21 μg/mL with the maximum up regulation of p53 and caspase 3 expression and the lowest Ki-67 level, relative to both functionalized CuNPs (FCNPs) and FeNPs (FFNPs). Meanwhile, it maintained the viability of normal human cells by EC100 up to 1999.7 μg/mL. Regarding the antimicrobial activity, FCFNCs offered > 70% growth reduction among wide spectrum prokaryotic and eukaryotic pathogens. Additionally, the synergistic feature of FCFNCs disintegrated the pre-established biofilm and algal growth in a dose-dependent manner. However, as a bio-sorbent, FCFNCs decolorized > 68% of malachite green and congo red dyes (200 mg/L), reflecting considerable remediation efficiency, confirmed by FTIR of FCFNCs- adsorbed dyes and microtoxicity/cytotoxicity of solutions after remediation. This study offers new insights into promising CuFe-hybrid nanocomposites for recruitment in several applications.

The Interplay of Autoclaving with Oxalate as Pretreatment Technique in the View of Bioethanol Production Based on Corn Stover

“…In the CS sample, the peak at 1234.9 cm −1 was related to guaiacyl/syringyl ring and C-O stretching vibration, which indicates the presence of both syringyl and guaiacyl in residual lignin and/or xylan [37]. This peak was shifted to 1230 cm −1 in CSOU sample, which could have been due to C = O absorption that resulted from cleavage of acetyl groups [38]. This peak wholly disappeared in the CSOA, which meant significant removal of lignin.…”

Role of Combined Na2HPO4 and ZnCl2 in the Unprecedented Catalysis of the Sequential Pretreatment of Sustainable Agricultural and Agro-Industrial Wastes in Boosting Bioethanol Production

“…After the pretreatment process, the intensity of these peaks is reduced, indicating lower lignin content. In the case of the CS sample, there is a peak at 1232 cm −1 , which corresponds to C-O stretching vibration and guaiacyl/syringyl ring in xylan and/or lignin [46]. This peak vanished in the pretreated samples CSZ and CSNZ, which means that the pretreatment leads to effective delignification for the CS sample.…”

Heavy metals bioremediation and water softening using ureolytic strains Metschnikowia pulcherrima and Raoultella planticola

“…The biomarker of the MICP approach is urease activity 29 ; thus, among 50 microbial strains, some of them identified and published in previous studies, 30,31 six strains (five bacteria and one yeast) designated as 46N, 39A, VIP, CNE2, EM3 and 29A were selected based on their higher urease activity. Such strains were identified as Alcaligenes faecalis, Bacillus aryabhattai, Raoultella planticola, Ochrobactrum sp., Streptomyces cyaneofuscatus and Metschnikowia pulcherrima, respectively.…”