Concurrent Quantitation of Total Campylobacter and Total Ciprofloxacin-Resistant Campylobacter Loads in Rinses from Retail Raw Chicken Carcasses from 2001 to 2003 by Direct Plating at 42°C

Abstract

This is the first report on the use of a normally lethal dose of ciprofloxacin in a Campylobacter agar medium to kill all ciprofloxacin-sensitive Campylobacter spp. but allow the selective isolation and quantitation of naturally occurring presumptive ciprofloxacin-resistant Campylobacter CFU in rinses from retail raw chicken carcasses (RTCC). Thermophilic-group total Campylobacter CFU and total ciprofloxacin-resistant Campylobacter CFU (irrespective of species) were concurrently quantified in rinses from RTCC by direct plating of centrifuged pellets from 10 or 50 ml out of 400-ml rinse subsamples concurrently on Campylobacter agar and ciprofloxacin-containing Campylobacter agar at 42°C (detection limit ‫؍‬ 0.90 log 10 CFU/carcass).

Campylobacter contamination in retail poultry meats and by-products in Japan: A literature survey

“…For example, the prevalence of Campylobacter spp. was reported to be 79.0% in the USA (Nannapaneni, Story, Wiggins, & Johnson, 2005), 62.4% in Canada (ValdiviesoGarcia et al, 2007), 50.5-73.5% in the UK (Meldrum, Griffiths, Smith, & Evans, 2005;Meldrum, Smith, & Wilson, 2006;Meldrum, Tucker, & Edwards, 2004;Meldrum, Tucker, Smith, & Edwards, 2005; Wilson, 2002;Wilson, 2003), and 32.0-43.0% in Germany (Adam, Contzen, Horlacher, & Rau, 2006;Hartung, 2006). In this review, we calculated that approximately 60% of raw poultry meats and by-products in Japan were contaminated with Campylobacter spp.…”

Prevalence and Characterization of Campylobacter jejuni Isolated from Pasture Flock Poultry

“…Isolation and enumeration of Campylobacter from retail carcasses were done as previously described (Nannapaneni and others 2005) with some modification. Briefly, each carcass was placed in a sterile bag and rinsed for 2 min with Butterfield's phosphate diluent (BPD; 6.8% KH 2 PO 4 ; 7.2 pH; 400 mL).…”

Characterization of Staphylococcus aureus Isolates from Retail Chicken Carcasses and Pet Workers in Northwest Arkansas

“…T he w orker handled sw ine carcasses on a daily basis, and the M RSA strain that infected the w orker w as associated w ith the swine carcasses. Because S. aureus is present on the skin o f many agriculturally im portant anim als, the risk o f carcass (12). Typical S. aureus colonies that displayed a halo were selected from each plate and confirmed by streaking onto chromogenic agar (CHROMagar, Paris, France), Gram staining, microscopic visualization, and agglutination reactions.…”