Prevalence and Characterization of <i>Campylobacter jejuni</i> Isolated from Pasture Flock Poultry

Hanning, Irene; Biswas, Debabrata; Herrera, Paul; Roesler, Mary; Ricke, Steven C.

doi:10.1111/j.1750-3841.2010.01747.x

Cited by 45 publications

(31 citation statements)

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“…The pldA and ciaB genes have been detected from C. jejuni poultry isolates at the frequencies of 63.6 and 67.3%, respectively (Melo et al, 2013). Hanning et al (2010) reported relatively low detection frequencies of ciaB (40%) and pldA (56%) in C. jejuni isolates from poultry carcasses. The virB11 gene is located in the virulence plasmid pVir, which is often detected in C. jejuni strains that cause bloody diarrhea (Bacon et al, 2002; Tracz et al, 2005).…”

Section: Discussionmentioning

confidence: 94%

Differential Survival of Hyper-Aerotolerant Campylobacter jejuni under Different Gas Conditions

McMullen

Chui

et al. 2017

Front. Microbiol.

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Campylobacter jejuni accounts for a significant number of foodborne illnesses around the world. C. jejuni is microaerophilic and typically does not survive efficiently in oxygen-rich conditions. We recently reported that hyper-aerotolerant (HAT) C. jejuni are highly prevalent in retail poultry meat. To assess the capabilities of HAT C. jejuni in foodborne transmission and infection, in this study, we investigated the prevalence of virulence genes in HAT C. jejuni and the survival in poultry meat in atmosphere at a refrigeration temperature. When we examined the prevalence of eight virulence genes in 70 C. jejuni strains from raw poultry meat, interestingly, the frequencies of detecting virulence genes were significantly higher in HAT C. jejuni strains than aerosenstive C. jejuni strains. This suggests that HAT C. jejuni would potentially be more pathogenic than aerosensitive C. jejuni. Under aerobic conditions, aerosensitive C. jejuni survived at 4°C in raw poultry meat for 3 days, whereas HAT C. jejuni survived in poultry meat for a substantially extended time; there was a five-log CFU reduction over 2 weeks. In addition, we measured the effect of other gas conditions, including N2 and CO2, on the viability of HAT C. jejuni in comparison with aerosensitive and aerotolerant strains. N2 marginally affected the viability of C. jejuni. However, CO2 significantly reduced the viability of C. jejuni both in culture media and poultry meat. Based on the results, modified atmosphere packaging using CO2 may help us to control poultry contamination with HAT C. jejuni.

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Section: Discussionmentioning

confidence: 94%

Differential Survival of Hyper-Aerotolerant Campylobacter jejuni under Different Gas Conditions

McMullen

Chui

et al. 2017

Front. Microbiol.

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“…This gene has also been detected in 28% of the C. jejuni strains isolated from free-range broiler flocks (Hanning et al, 2010) and with a low prevalence for C. coli from various origins (Wieczorek and Osek, 2010, 2013; Acik et al, 2013). The low frequency of these isolates did not allow us to conclude that their presence in organic farms is related to the management of this type of production system.…”

Section: Discussionmentioning

confidence: 97%

No Clear Differences between Organic or Conventional Pig Farms in the Genetic Diversity or Virulence of Campylobacter coli Isolates

et al. 2017

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To evaluate the impact of pig farm management on the genetic diversity and on the virulence of Campylobacter coli, we characterized isolates from 19 organic pig farms (62 isolates) and from 24 conventional pig farms (58 isolates). The 120 C. coli isolates were typed using pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) and the presence of nine virulence genes was screened using real-time PCR. The capacity of adhesion and invasion of 61 isolates (32 from organic and 29 from conventional farms) were then tested on human intestinal Caco-2 cells. A total of 59 PFGE types and of 50 sequence types (STs) were identified. Twelve PFGE types and nine STs, accounting for 34 and 41.6% of the isolates, respectively, were common between the two production systems with ST854 dominating (18.3% of the isolates). Twenty-nine PFGE types and 25 STs were only found in isolates from organic farms, and 18 PFGE types and 16 STs from conventional farms. No significant differences were found in diversity despite the differences in rearing systems, except at the locus level for the glnA, gltA, and uncA genes. All isolates, regardless of their origin, carried the ceuE, iam, ciaB, and flaA genes and more than 95% of the isolates carried the cadF and cdtABC genes. No significant differences were found in pathogenicity between the two farming systems. The pathogenicity of the C. coli isolates was low compared to C. jejuni control strains tested. The plasmid gene virb11 was detected in only 13 isolates from organic farms; these isolates showed greater invasion capacity than those without this gene. Our study indicates that pig farm management does not significantly affect the diversity and the virulence of Campylobacter coli isolated from pigs. The common genotypes between conventional and organic farms may indicate that some genotypes are adapted to pigs.

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“…As a consequence, only few such investigations have been performed, and it is difficult to judge how representative these studies are for other flocks or other slaughterhouses (Hanning et al . ; Kudirkiene et al . ).…”

Section: Introductionmentioning

confidence: 99%

The effect of different isolation protocols on detection and molecular characterization of Campylobacter from poultry

Ugarte‐Ruiz

Wassenaar

Gómez-Barrero

et al. 2013

Lett Appl Microbiol

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Significance and Impact of Study: The tracing of Campylobacter through the food chain remains important to control campylobacteriosis in humans. Our study points out that the isolation method used affects the genotypes obtained, and this should be considered as a variant when comparing the results of surveillance studies. AbstractWe determined whether different methods to isolate Campylobacter (including the ISO standard 10272:2006-1) affected the genotypes detectable from poultry, at three points during slaughter: caecal content, neck skin and meat. Carcasses from 28 independent flocks were thus sampled (subset A). In addition, ten neck skin samples from four flocks, ten caecal samples from ten different flocks and ten unrelated meat samples obtained from local supermarkets were collected (subset B). Campylobacter was isolated using eight different protocols: with and without enrichment using Bolton broth, Preston broth or Campyfood broth (CFB), followed by culture on either modified Charcoal Cefoperazone Deoxycholate Agar (mCCDA) or Campyfood agar (CFA). All obtained isolates were genotyped for flaA-SVR, and over half of the isolates were also typed by MLST. The strain richness, as a measure of number of detected fla-genotypes, obtained from subset A neck skin and caecal samples was higher than that of meat samples. In half of the cases, within a flock, at least one identical fla-genotype was obtained at all three slaughter stages, suggestive of autologous contamination of carcasses. Enrichment reduced the observed richness of isolates, while CFA plates increased richness compared to mCCDA plates, irrespective of inclusion of an enrichment step. Because the isolation protocol used influences both the yield and the fla-genotype richness obtained from poultry, this variable should be taken into account when different studies are being compared.

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Prevalence and Characterization of Campylobacter jejuni Isolated from Pasture Flock Poultry

Cited by 45 publications

References 50 publications

Differential Survival of Hyper-Aerotolerant Campylobacter jejuni under Different Gas Conditions

Differential Survival of Hyper-Aerotolerant Campylobacter jejuni under Different Gas Conditions

No Clear Differences between Organic or Conventional Pig Farms in the Genetic Diversity or Virulence of Campylobacter coli Isolates

The effect of different isolation protocols on detection and molecular characterization of Campylobacter from poultry

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