Conceptual Design of Micro-Bioreactors and Organ-on-Chips for Studies of Cell Cultures

Mandenius, Carl‐Fredrik

doi:10.3390/bioengineering5030056

Cited by 33 publications

(27 citation statements)

References 87 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The OOAC involves four key components, including (1) microfluidics; (2) living cell tissues; (3) stimulation or drug delivery; and (4) sensing [45]. The microfluidic component refers to the use of microfluidics to deliver target cells to a pre-designated location and includes a system of culture fluid input and waste liquid discharge during the culture process.…”

Section: Key Componentsmentioning

confidence: 99%

Organ-on-a-chip: recent breakthroughs and future prospects

et al. 2020

View full text Add to dashboard Cite

BackgroundMicrofluidics is a science and technology that precisely manipulates and processes microscale fluids. It is commonly used to precisely control microfluidic (10 −9 to 10 −18 L) fluids using channels that range in size from tens to hundreds of microns and is known as a "lab-on-a-chip" [1][2][3][4]. The microchannel is small, but has a large surface area and high mass transfer, favoring its use in microfluidic technology applications including low regent usage, controllable volumes, fast mixing speeds, rapid responses, and precision control of physical and chemical properties [1,5,6]. Microfluidics integrate sample preparation, reactions, separation, detection, and basic operating units such as cell culture, sorting and cell lysis [7]. For these reasons, interest in OOAC has intensified [8]. OOAC combines a range of chemical, biological and material science disciplines [9] and was selected as one of the "Top Ten Emerging Technologies" in the World Economic Forum [10].OOAC is a biomimetic system that can mimic the environment of a physiological organ, with the ability to regulate key parameters including AbstractThe organ-on-a-chip (OOAC) is in the list of top 10 emerging technologies and refers to a physiological organ biomimetic system built on a microfluidic chip. Through a combination of cell biology, engineering, and biomaterial technology, the microenvironment of the chip simulates that of the organ in terms of tissue interfaces and mechanical stimulation. This reflects the structural and functional characteristics of human tissue and can predict response to an array of stimuli including drug responses and environmental effects. OOAC has broad applications in precision medicine and biological defense strategies. Here, we introduce the concepts of OOAC and review its application to the construction of physiological models, drug development, and toxicology from the perspective of different organs. We further discuss existing challenges and provide future perspectives for its application.

show abstract

Section: Key Componentsmentioning

confidence: 99%

Organ-on-a-chip: recent breakthroughs and future prospects

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Biological functions and requirements include: (a) nature of the cells (CHO or HEK cells) with concentration ranging from 10,000 to 10,000,000, (b) expression of proteins such as IgGs, (c) serum-free medium, and (d) culture time of 7-14 days [15].…”

Section: Microbioreactors To Produce Monoclonal Antibodiesmentioning

confidence: 99%

“…Technical information parameters must include: (a) shaking for better mixing, (b) oxygen transfer value >100 h −1 to aerate the culture so that it can be extended to a large-scale process, (c) oxygen permeability <1%, (d) surface hydrophobicity (10°) along with a confocal microscope installed in situ without disturbing the reaction, (e) cell culture volume, (f) transportation of culture media to contained culture, and (g) measurement of chemical and physical conditions of culture and media [15].…”

Section: Microbioreactors To Produce Monoclonal Antibodiesmentioning

confidence: 99%

“…This is a part of Hubka-Eder map [16] focused on biological and technical functions. Hubka-Eder map shows the importance of integration among the subsystems, that is, the expression systems, cell line, medium with various technical and information functions for the design of the microbioreactors for the maximum production of monoclonal antibodies [15].…”

Section: Microbioreactors To Produce Monoclonal Antibodiesmentioning

confidence: 99%

See 1 more Smart Citation

Microbioreactors and Perfusion Bioreactors for Microbial and Mammalian Cell Culture

Ravindran¹,

Singh²,

Nene³

et al. 2019

Biotechnology and Bioengineering

View full text Add to dashboard Cite

Screening for novel producer strains and enhanced therapeutic production at reduced cost has been the focus of most of the biopharmaceutical industries. The obligation to carry out prolonged intensive pilot scale experiments gave birth to micro-scale bioreactor systems. Screening large number of microorganisms using shake flasks and benchtop bioreactors is tedious and consumes resources. Microbioreactors that mimic benchtop bioreactors are capable not only of high throughput screening of producer strains, but also aid in optimizing the growth kinetics and expression of proteins. Modern technology has enabled the collection of precise online data for variables such as optical density (OD), pH, temperature, dissolved oxygen (DO), and adjusting in mixing inside microreactors. Microbioreactors have become an irreplaceable tool for biochemical engineers and biotechnologists to perform a large number of experiments simultaneously. Another aspect that is vital to any industry is the product yield and subsequent downstream processing. Perfusion bioreactors are one of the upcoming advances in bioreactor systems that have the potential to revolutionize biologics production. This chapter intends to take a review of different aspects of microbioreactors and perfusion bioreactors including their potential in high throughput pilot studies and microbial and mammalian cell cultivation technologies.

show abstract

“…The fundamental design challenges in MBRs are highlighted in two review contributions [ 6 , 7 ]. The similarities of the design of different MBRs, despite purposes, lead to a general design methodology for MBRs where functionality drives the design [ 6 ]. The engineering-based design of MBRs and OoC devices can take advantage of established design science theory, in which a systematic evaluation of functional concepts and user requirements takes place.…”

mentioning

confidence: 99%