Advances in Micro-Bioreactor Design for Organ Cell Studies

Mandenius, Carl‐Fredrik

doi:10.3390/bioengineering5030064

Cited by 2 publications

(3 citation statements)

References 15 publications

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“…On starvation experiment, serum‐containing medium was removed, and cells were washed twice with sterile phosphate buffer saline (PBS). Then, each 2 × 10 5 /100 μL of L428‐GFP and L428‐LMP1 cells were serum starved in 2.5% FBS for up to 72 hours 22 …”

Section: Methodsmentioning

confidence: 99%

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Epstein‐Barr virus latent membrane protein‐1 upregulates autophagy and promotes viability in Hodgkin lymphoma: Implications for targeted therapy

et al. 2021

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Hodgkin lymphoma (HL) is composed of neoplastic Hodgkin and Reed-Sternberg cells in an inflammatory background. The neoplastic cells are derived from germinal center B cells that, in most cases, are infected by Epstein-Barr virus (EBV), which may play a role in tumorigenesis. Given that EBV-latent membrane protein 1 (LMP1) regulates autophagy in B cells, we explored the role of autophagy mediated by EBV or LMP1 in HL. We found that EBV-LMP1 transfection in HL cells induced a modest increase in autophagy signals, attenuated starvation-induced autophagic stress, and alleviated autophagy inhibition-or doxorubicin-induced cell death. LMP1 knockdown leads to decreased autophagy LC3 signals. A xenograft mouse model further showed that EBV infection significantly increased expression of the autophagy marker LC3 in HL cells. Clinically, LC3 was expressed in 15% (19/127) of HL samples, but was absent in all cases of nodular lymphocyte-predominant and lymphocyte-rich classic HL cases. Although expression of LC3 was not correlated with EBV status or clinical outcome, autophagic blockade effectively eradicated LMP1-positive HL xenografts with better efficacy than LMP1-negative HL xenografts. Collectively, these results suggest that EBV-LMP1 enhances autophagy and promotes the viability of HL cells.This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Section: Methodsmentioning

confidence: 99%

“…Then, each 2 × 10 5 /100 μL of L428-GFP and L428-LMP1 cells were serum starved in 2.5% FBS for up to 72 hours. 22 Autophagic inhibition may be a potential therapeutic strategy for treating patients with HL, especially EBV-positive cases.…”

Section: Hl Cell Lines and Lymphoblastoid Cell Linesmentioning

confidence: 99%

Epstein‐Barr virus latent membrane protein‐1 upregulates autophagy and promotes viability in Hodgkin lymphoma: Implications for targeted therapy

et al. 2021

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“…The generation of an organoid provides a complex structure similar to its biological counterpart. Unlike the current organ transplantation treatment approach, such autologous tissue is not subject to immune rejection; however, the formation of such complex organoid structures requires the application of bioreactors [5] .…”

Section: Introductionmentioning

confidence: 99%

Research Update on Bioreactors Used in Tissue Engineering

Wang

Deng

et al. 2021

J. Shanghai Jiaotong Univ. (Sci.)

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Owing to the paucity of donor organs and their acute rejection by the immune system after transplantation, advanced organ failure is one of the major challenges faced by the medical community. Static culturing used for synthesising tissues and organs cannot simulate the in vivo mechanical and biochemical signals; therefore, such artificial organs fail to maintain effective functional activity following transplantation. Tissue engineering can overcome these hurdles by successfully enabling regeneration of tissues and organs in vitro. Bioreactors are pivotal in the development and generation of engineered biological products. They simulate the in vivo microenvironment of tissue growth while also providing various mechanical stimuli and biochemical signals to stem cells to effectively generate transplantable organs or tissues. Various designs and types of bioreactors, their applications, and future research prospects are summarised, which promote functional tissue engineering.

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Advances in Micro-Bioreactor Design for Organ Cell Studies

Abstract: The engineering design of microbioreactors (MBRs) and Organ-on-Chips (OoCs) has advanced considerably in recent years [...]

Cited by 2 publications

References 15 publications

Epstein‐Barr virus latent membrane protein‐1 upregulates autophagy and promotes viability in Hodgkin lymphoma: Implications for targeted therapy

Epstein‐Barr virus latent membrane protein‐1 upregulates autophagy and promotes viability in Hodgkin lymphoma: Implications for targeted therapy

Research Update on Bioreactors Used in Tissue Engineering

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