Microbioreactors and Perfusion Bioreactors for Microbial and Mammalian Cell Culture

Ravindran, Selvan; Singh, Pooja; Nene, Sanjay; Rale, Vinay; Mhetras, Nutan; Vaidya, Anuradha

doi:10.5772/intechopen.83825

Cited by 8 publications

(5 citation statements)

References 30 publications

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“…Our current study shows the potential of enhancing cell retention capacity by microfluidic devices in the elasto-inertial fluid regime, as demonstrated by Figure 5. Compared with other membrane-less cell manipulation techniques such as acoustic wave separators, centrifuges, hydrocyclones, and gravity settlers, [51,52] (Table S1, Supporting Information), the microfluidic cell retention device based on elasto-inertial cell focusing has many benefits. With its enhanced cell density capacity demonstrated in this study, the membrane-less microfluidic device can perform cell retention with advantages including removal of small dead cells/debris and low capital/operational expenses, [49,50,[53][54][55] which could lead to efficient and reliable high-density perfusion culture at various scales.…”

Section: Discussionmentioning

confidence: 99%

Separation of Ultra‐High‐Density Cell Suspension via Elasto‐Inertial Microfluidics

Kwon

Choi

Han

2021

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Separation of high‐density suspension particles at high throughput is crucial for many chemical, biomedical, and environmental applications. In this study, elasto‐inertial microfluidics is used to manipulate ultra‐high‐density cells to achieve stable equilibrium positions in microchannels, aided by the inherent viscoelasticity of high‐density cell suspension. It is demonstrated that ultra‐high‐density Chinese hamster ovary cell suspension (>26 packed cell volume% (PCV%), >95 million cells mL−1) can be focused at distinct lateral equilibrium positions under high‐flow‐rate conditions (up to 10 mL min−1). The effect of flow rates, channel dimensions, and cell densities on this unique focusing behavior is studied. Cell clarification is further demonstrated using this phenomenon, from 29.7 PCV% (108.1 million cells mL−1) to 8.3 PCV% (33.2 million cells mL−1) with overall 72.1% reduction efficiency and 10 mL min−1 processing rate. This work explores an extreme case of elasto‐inertial particle focusing where ultra‐high‐density culture suspension is efficiently manipulated at high throughput. This result opens up new opportunities for practical applications of high‐particle‐density suspension manipulation.

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Section: Discussionmentioning

confidence: 99%

Separation of Ultra‐High‐Density Cell Suspension via Elasto‐Inertial Microfluidics

Kwon

Choi

Han

2021

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“…Additionally, in industry, MBRs are widely used to manufacture pharmaceuticals, chemicals, enzymes, and food from cell factories 6,12 .…”

Section: Micro Bioreactorsmentioning

confidence: 99%

Lab on a Chip: Bioreactors miniaturization for rapid optimization of biomedical processes and its impact on SARS-CoV-2 diagnosis

Ortega

Toledo

Santillán

et al. 2021

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Lab on a Chip (LoC) as part of Microbioreactors (MBRs) constitute an emergent technology to carry out micro-bioprocesses based on microfluidics research. In this review, the usefulness of LoCs is exposed since its inception, demonstrating that it is a multidisciplinary research field, gathering different science branches to develop this technology. As a result, a beneficial point of advancement is reached, producing useful consumables for humanity. Some of the described LoCs throughout this work are also used to detect infectious diseases caused by bacteria or viruses, allowing accelerated studies on emerging or high-impact diseases, such as COVID-19. Here are also displayed with an updated panorama, different strategies to improve the use, applications in the biomedical field, and spread of these devices aimed at their availability to solve social problems.

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“…Upon successful completion of pharmacological and toxicological studies, the desired phytochemicals can be scaled up using bioreactors with the aid of microorganisms [17] or a methodology can be developed to synthesize the compounds by biological methods [18].…”

Section: Drug Metabolismmentioning

confidence: 99%

Metabolism of Phytochemicals

Dixit¹,

Tiwari²,

Bose³

et al. 2021

Drug Metabolism

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Several phytochemicals have been developed as medicinal compounds. Extensive research has recently been conducted on phytochemicals such as curcumin, resveratrol, catechin, gallic acid, humulone, quercetin, rutin, diosgenin, allicin, gingerenone-A, caffeic acid, ellagic acid, kaempferol, isorhamnetin, chlorogenic acid, and others. All of these phytochemicals are metabolized in the biological system. To study the metabolic pathways of phytochemicals, studies are done using both in vitro and in vivo techniques. Metabolism is critical in determining phytochemical bioavailability, pharmacokinetics, and effectiveness. Metabolism can occur in organs such as the intestine, liver, gut, and spleen. The metabolic process is aided by a variety of enzymes, including cytochrome P450 enzymes found in the organs. This study outlines a few phytochemicals metabolic pathways. Tannic acid, ellagic acid, curcumin, quercetin, and resveratrol are selected and explained as examples.

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Microbioreactors and Perfusion Bioreactors for Microbial and Mammalian Cell Culture

Cited by 8 publications

References 30 publications

Separation of Ultra‐High‐Density Cell Suspension via Elasto‐Inertial Microfluidics

Separation of Ultra‐High‐Density Cell Suspension via Elasto‐Inertial Microfluidics

Lab on a Chip: Bioreactors miniaturization for rapid optimization of biomedical processes and its impact on SARS-CoV-2 diagnosis

Metabolism of Phytochemicals

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