Concentration regimes of solutions of levan polysaccharide from Bacillus sp.

“…14 Nuclear magnetic resonance uses 1 H, 13 C, 15 N and 31 P as tracer atoms for spatial information about the structure. Nuclear magnetic resonance study is widely used to elucidate the conformational aspects of polysaccharide structure.…”

Section: Resultsmentioning

confidence: 99%

Kheri (Acacia chundra, family: Mimosaceae) gum: Characterization using analytical, mathematical and pharmaceutical approaches

Malviya

Sharma

Dubey³

2018

Polim. Med.

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“…Levan is one of two main types of fructans consisting mainly of ␤-2,6-D-fructosyl-fructose links in the main chain and ␤-2,1-Dfructosyl-fructose links in the branch chain (31). Levanase is a fructofuranosyl hydrolase, which hydrolyzes both the ␤-2,6-and ␤-2,1-D-fructosyl-fructose links in levan.…”

Section: Discussionmentioning

confidence: 99%

Efficient Simultaneous Saccharification and Fermentation of Inulin to 2,3-Butanediol by Thermophilic Bacillus licheniformis ATCC 14580

Chen

et al. 2014

Appl Environ Microbiol

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b 2,3-Butanediol (2,3-BD) is an important starting material for the manufacture of bulk chemicals. For efficient and large-scale production of 2,3-BD through fermentation, low-cost substrates are required. One such substrate, inulin, is a polydisperse fructan found in a wide variety of plants. In this study, a levanase with high inulinase activity and high pH and temperature stability was identified in Bacillus licheniformis strain ATCC 14580. B. licheniformis strain ATCC 14580 was found to efficiently produce 2,3-BD from fructose at 50°C. Then, the levanase was used for simultaneous saccharification and fermentation (SSF) of inulin to 2,3-BD. A fed-batch SSF yielded 103.0 g/liter 2,3-BD in 30 h, with a high productivity of 3.4 g/liter · h. The results suggest that the SSF process developed with the thermophilic B. licheniformis strain used might be a promising alternative for efficient 2,3-BD production from the favorable substrate inulin.

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“…I t i s re port ed th at l e van sucr a se a c ti vi ty i s in vo l ve d in a va ri ety of pro c es s es including survival of bacteria in soil (B. subtilis), phytopathogenesis (Erwinia and Pseudomonas species) and symbiosis (Paenibacillus polymyxa) of plant interactive bacteria (Hettwer et al,1995). Bacillus subtilis, known as the hay bacillus or grass bacillus, is a Gram-positive, catalase positive bacterium commonly found in soil (Madigan & Martinko, 2005 Levan is one of two main types of fructans, which are natural homopolymers of fructose (Arvidson et al 2006). It is a naturally occurring polymer of β-D-fructofuranose with β (26) linkages between repeating five-member fructofuranosyl rings and branching at C-1 (Arvidson et al 2006, Barone andMedynets., 2007).…”

Section: Introductionmentioning

confidence: 99%

“…Levans from plants generally have molecular weights about 2000 -33.000Da (Rhee et al, 2002). The molecular weight of levan, and the fraction of residues incorporated in side chains, depends on both the source and the growth conditions, with plant levan and microbially-produced levan having very different characteristics (Arvidson et al 2006;Newbrun 1971;Stivala, and Bahary 1978;Huber et al, 1994). Recently it was reported in the B. subtilis NRC1aza levansucrase, the unique feature of this isolate its ability to produce two types of levan with different molecular weights (El Fattah et al, 2012).…”

Section: Introductionmentioning

confidence: 99%