IntroductionThe aim of the present research was to formulate chitosan‐kheri gum polyelectrolyte complex (CKGPEC) stabilized etoricoxib containing cubic nanoparticles and evaluate against various human cancer cell lines.MethodsThe novel solvent‐antisolvent method was utilized for the fabrication of nanoparticles using CKGPEC as a stabilizer. 32 factorial designs were applied to investigate the effect of concentration of chitosan (Ch) and kheri gum (KG) over entrapment efficiency and size of nanoparticles. Nanoparticles were characterized and evaluated against the human breast cancer cell line (MCF7), human colon cancer cell line (HT‐29), and human skin cancer cell lines (SK‐MEL‐2).ResultsIt was observed that the concentration of Ch and KG significantly affects the entrapment efficiency and size of nanoparticles. The entrapment efficiency of nanoparticles was found in the range of 70.21 ± 0.42% (K4) to 82.77 ± 0.29% (K6) while the size was observed 79.3 nm (K5) to 490.1 nm (K1). SEM clearly showed the cubic shape of nanoparticles. All the formulations followed Baker‐Lonsdale kinetic model of drug release. The utilization of the egg membrane and tomato membrane as a biological barrier was not altering the release kinetics of the drug. Prepared nanoparticles were found to be effective against various human cancer cells but the better effect was observed against SK‐MEL‐2 cells than MCF‐7 cells, followed by HT‐29 cell in in vitro conditions.DiscussionTaken together, it can be concluded that Ch‐KG PEC stabilized nanoparticles were successfully formulated and could be utilized against various human cancer. In future clinical studies could be performed for the exact determination of therapeutic potential.
Objective. The study was designed to evaluate pharmacological potential of hydroalcoholic leaves extract of Chrozophora tinctoria intended for wound healing in diabetic rats' model. Methods. The method used to evaluate the pharmacological potential of hydroalcoholic leave extract was physical incision rat model. In this model, cutting of the skin and/or other tissues with a sharp blade has been made and the rapid disruption of tissue integrity with minimal collateral damage was observed shortly. Animals used in the study were divided into four groups that consist of six animals in each group. Group I serves as normal control, Group II serves as disease control, Group III was used as standard treatment (Povidone iodine 50 mg/kg b.w.), and Group IV was used for test drug (C. tinctoria 50 mg/kg b.w.). Result. The hydroalcoholic leave extract of Chrozophora tinctoria has been significantly observed to heal the wound (98%) in diabetic rats within 21 days, while standard drug (Povidone iodine) healed the wound about 95% in the same condition. The oral dose (50 mg/kg b.w.) of Chrozophora tinctoria was also found to improve the elevated blood glucose level in comparison to disease control group, which increased after the oral administration of Streptozotocin. Conclusion. The Chrozophora tinctoria has significant wound healing potential in the animal having physically damaged tissue in diabetic condition.
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