Concentration, characterization and application of lipases from Sporidiobolus pararoseus strain

Smaniotto, Alessandra; Skovronski, Aline; Rigo, Elisandra; Tsai, Siu Mui; Durrer, Ademir; Foltran, Lillian Liva; Paroul, Natália; Luccio, Marco Di; Oliveira, J. Vladimir; Oliveira, Débora de; Treichel, Helen

doi:10.1590/s1517-83822014000100043

Cited by 19 publications

(7 citation statements)

References 30 publications

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“…The material retained on the filter were frozen at −80°C (24 h) and lyophilized by 24 h being named as the lyophilized fungus (Smaniotto et al . ).…”

Section: Methodsmentioning

confidence: 97%

See 1 more Smart Citation

Enantioselective biotransformation of sterically hindered amine substrates by the fungus Stemphylium lycopersici

Queiroz¹,

Pinheiro²,

Sutili

et al. 2018

J Appl Microbiol

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“…The material retained on the filter were frozen at −80°C (24 h) and lyophilized by 24 h being named as the lyophilized fungus (Smaniotto et al . ).…”

Section: Methodsmentioning

confidence: 97%

“…After 5 days of fermentation at 32Á5°C and 120 rev min À1 , samples were collected and filtered through a Whatmann paper (0Á45 mm). The material retained on the filter were frozen at À80°C (24 h) and lyophilized by 24 h being named as the lyophilized fungus (Smaniotto et al 2014).…”

Section: Fungus and Crude Enzymatic Extract: Additional Biotransformamentioning

confidence: 99%

Enantioselective biotransformation of sterically hindered amine substrates by the fungus Stemphylium lycopersici

Queiroz¹,

Pinheiro²,

Sutili

et al. 2018

J Appl Microbiol

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“…However, this waste or byproducts are rich in nutrients as proteins, carbohydrates, lipids and minerals salts that can easily metabolized by microbial strains. (8) In this study, we reported the production of a lipase by a new strain of Pseudomonas sp. in medium containing residual chicken fat and soybean oil residues as the only carbon source.…”

Section: Introductionmentioning

confidence: 99%

Utilization of agroindustrial byproducts for the production of lipase by a new strain of Pseudomonas sp.

Baldo

Baggio

Oliveira

et al. 2020

Semin. Cienc. Biol. Saude

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Este estudo teve como objetivo avaliar a produção de lipases por uma nova cepa de Pseudomonas sp. utilizando meio de fermentação contendo subprodutos de industrialização de carne de frango e óleo de soja. Os resultados indicaram que a gordura de frango e a goma de soja induziram 48,3 U/mL e 93,3 U/ml de atividade lipásica, respectivamente. No entanto, a produção de lipase mais elevada foi obtida quando a goma de lecitina bruta foi utilizada, induzindo 272,6 U/ml de atividade após 24 horas. A caracterização bioquímica parcial da enzima mostrou que as condições de reação ótimas foram de pH 9,0 e 35°C. A enzima foi estável nas temperaturas entre 25 a 75°C e pH de 6 a 9. A enzima mostrou boa estabilidade em solventes orgânicos tais como acetonitrila, hexano, etanol e isopropanol. Este estudo indicou que os subprodutos testados são promissores para a produção de lipase e podem contribuir para a redução dos custos de produção enzimática em larga escala, aumentar o valor desses subprodutos e reduzir potenciais impactos ambientais causados por sua acumulação na natureza.

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“…There are many applications of lipases in industry, especially in biodiesel production. They are also useful in lipolysis with specific application domains, including the manufacture of dairy, oil and fat, food and flavour, bioremediation, and production of leather products [2][3][4].…”

Section: Introductionmentioning

confidence: 99%

Production of Thermostable T1 Lipase Using Agroindustrial Waste Medium Formulation

et al. 2018

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Large-scale production of T1 lipase using conventional culture media is costly. To reduce the cost of production, an alternative growth medium using local resources has been developed. In this study, the growth of recombinant Escherichia coli and expression of T1 lipase were tested using different agroindustrial wastes as carbon and nitrogen sources by conventional method. Subsequently, by using central composite rotatable design (CCRD), a set of 30 experiments was generated to evaluate the effect of different parameters, including the amount of molasses (as carbon source), fish waste (as nitrogen source), NaCl, and inducer concentration on production of T1 lipase. Response surface methodology (RSM) analysis indicated that all factors had significant effects on T1 lipase production. This statistical analysis was utilised to develop a quadratic model to correlate various important variables for the growth of the recombinant strain and regulation of gene expression to the response (T1 lipase activity). Optimum conditions for T1 lipase production were observed to be 1.0 g/L of molasses, 2.29 g/L of fish waste, 3.46 g/L of NaCl, and 0.03 mM of IPTG (Isopropyl β-d-1-thiogalactopyranoside). Based on these conditions, the actual lipase activity was found to be 164.37 U/mL, which fitted well with the maximum predicted value of 172.89 U/mL. Therefore, the results demonstrated that, the statistical analysis, performed using RSM, was efficient in optimising T1 lipase production. Moreover, the optimum conditions obtained can be applied to scale up the process and minimise the cost of enzyme production.

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Concentration, characterization and application of lipases from Sporidiobolus pararoseus strain

Cited by 19 publications

References 30 publications

Enantioselective biotransformation of sterically hindered amine substrates by the fungus Stemphylium lycopersici

Enantioselective biotransformation of sterically hindered amine substrates by the fungus Stemphylium lycopersici

Utilization of agroindustrial byproducts for the production of lipase by a new strain of Pseudomonas sp.

Production of Thermostable T1 Lipase Using Agroindustrial Waste Medium Formulation

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