Concentration and selective separation of bioactive peptides from an alfalfa white protein hydrolysate by electrodialysis with ultrafiltration membranes

Leaf protein concentration of alfalfa juice by membrane technology

“…After drying green crop, alfalfa is utilized as raw material for the production of fodder pellets for cattle, due to its high feed value and high crude proteins content (about 2600 kg/ha) [2]. During the pellet production process, the green crop of alfalfa is chopped and pressed before drying, while much alfalfa juice is generated.…”

“…This green extracted juice containing high proteins content has been recognized as an effective source of high quality proteins for animals and human consumption, because of abundant sources, high nutritive value and absence of animal cholesterol [3]. Alfalfa leaf protein includes about 50% hydrophilic proteins and 50% lipophilic proteins [2]. Hydrophilic proteins have high digestibility and a balanced aminogram, which possesses significant functional properties, such as emulsifying, jellifying and foaming agents [4][5][6].…”

Concentration and selective fractionation of an antihypertensive peptide from an alfalfa white proteins hydrolysate by mixed ion-exchange centrifugal partition chromatography

“…The content of its dry matter in peptide, assessed from a Kjeldahl analysis, was about 95% (w/w). This complex hydrolysate, containing more than one hundred peptides [23], is known to have a significant in vivo anti-hypertensive activity after oral administration on an animal model (spontaneously hypertensive rats) [2]. It was hypothesized that this bioactivity was due to the presence of the potent ACE inhibitory peptide VW [23].…”

“…This purification strategy was applied to the capture of the l-Val-l-Trp (VW) antihypertensive dipeptide from an alfalfa (Medicago sativa, Fabaceae) white protein hydrolysate. This hydrolysate, composed of more than one hundred different peptides [23] was shown to have a strong in vivo antihypertensive activity [2], probably due to presence of the potent anti-angiotensin converting enzyme (anti-ACE) peptide VW [23]. …”

Insertion of Filtration Membranes into Electrodialysis Stack and Its Impact on Process Performance

“…for separation of chitosan oligomers [1,6], cranberry juice enrichment by polyphenol antioxidants [7,8], separation of whey proteins [3], separation of bioactive peptides from alfalfa protein hydrolysate [9,10], soy protein hydrolysate [11] or snow crab protein hydrolysate [12,13].…”