Concentration and selective fractionation of an antihypertensive peptide from an alfalfa white proteins hydrolysate by mixed ion-exchange centrifugal partition chromatography

Boudesocque, Leslie; Kapel, Romain; Paris, Cédric; Dhulster, Pascal; Marc, Ivan; Renault, Jean‐Hugues

doi:10.1016/j.jchromb.2012.07.034

Cited by 18 publications

(9 citation statements)

References 22 publications

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“…Ion exchange chromatography is the most popular method for purification of ions and polar molecules, such as proteins, peptides, and other charged biomolecules [ 27 ]. Separation using ion exchange chromatography depends upon the reversible adsorption of charged solute molecules to immobilize ion exchange groups of opposite charge, and the adsorption capacity was positively correlated with the charge number of isolated molecules.…”

Section: Resultsmentioning

confidence: 99%

Bioactive Peptides from Cartilage Protein Hydrolysate of Spotless Smoothhound and Their Antioxidant Activity In Vitro

et al. 2018

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In the experiment, crude proteins from spotless smoothhound (Mustelus griseus), cartilages were isolated by HCl-Guanidine buffer, and its hydrolysate was prepared using trypsin at pH 8.0, 40 °C with a total enzyme dose of 2.5%. Subsequently, three antioxidant peptides were purified from the hydrolysate using membrane ultrafiltration, anion-exchange chromatography, gel filtration chromatography, and reverse phase high-performance liquid chromatography. The amino acid sequences of isolated peptides were identified as Gly-Ala-Glu-Arg-Pro (MCPE-A); Gly-Glu-Arg-Glu-Ala-Asn-Val-Met (MCPE-B); and Ala-Glu-Val-Gly (MCPE-C) with molecular weights of 528.57, 905.00, and 374.40 Da, respectively, using protein amino acid sequence analyzer and mass spectrum. MCPE-A, MCPE-B and MCPE-C exhibited good scavenging activities on 2,2-diphenyl-1-picrylhydrazyl radicals (DPPH•) (EC50 3.73, 1.87, and 2.30 mg/mL, respectively), hydroxyl radicals (HO•) (EC50 0.25, 0.34, and 0.06 mg/mL, respectively), 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid radicals (ABTS+•) (EC50 0.10, 0.05, and 0.07 mg/mL, respectively) and superoxide anion radicals (normalO2−•) (EC50 0.09, 0.33, and 0.18 mg/mL, respectively). MCPE-B showed similar inhibiting ability on lipid peroxidation with butylated hydroxytoluene (BHT) in a linoleic acid model system. Furthermore, MCPE-A, MCPE-B, and MCPE-C could protect H2O2-induced HepG2 cells from oxidative stress by decreasing the content of malonaldehyde (MDA) and increasing the levels of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and glutathione reductase (GSH-Rx). Glu, Gly, Met, and Pro in their sequences and low molecular weight could be attributed to the antioxidant activities of three isolated peptides. These results suggested that GAERP (MCPE-A), GEREANVM (MCPE-B), and AEVG (MCPE-C) from cartilage protein hydrolysate of spotless smoothhound might serve as potential antioxidants and be used in the pharmaceutical and health food industries.

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Section: Resultsmentioning

confidence: 99%

Bioactive Peptides from Cartilage Protein Hydrolysate of Spotless Smoothhound and Their Antioxidant Activity In Vitro

et al. 2018

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“…Other purification techniques, such as capillary electrophoresis, capillary isoelectric focusing, counter-current chromatography and centrifugal partition chromatography, have also been used for peptide purification [ 10 , 116 , 117 , 118 , 119 , 120 , 121 ].…”

Section: Recovery and Purification Processmentioning

confidence: 99%

A Review of the Latest Advances in Encrypted Bioactive Peptides from Protein-Rich Waste

Lemes

Sala

Ores

et al. 2016

IJMS

181

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Bioactive peptides are considered the new generation of biologically active regulators that not only prevent the mechanism of oxidation and microbial degradation in foods but also enhanced the treatment of various diseases and disorders, thus increasing quality of life. This review article emphasizes recent advances in bioactive peptide technology, such as: (i) new strategies for transforming bioactive peptides from residual waste into added-value products; (ii) nanotechnology for the encapsulation, protection and release of controlled peptides; and (iii) use of techniques of large-scale recovery and purification of peptides aiming at future applications to pharmaceutical and food industries.

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“…Some new and potent antihypertensive peptides have been isolated from many plant food proteins. For example, a valine-and tryptophanrich peptide with high in vivo antiangiotensin converting enzyme (anti-ACE) activity was identified and purified from alfalfa white protein concentrate hydrolysate 40 . Further, four potent ACE inhibitory peptides (Ile-Tyr, Arg-Ile-Tyr, Val-Trp and Val-Trp-Ile-Ser), have been isolated from subtilisin digest of rapeseed protein.…”

Section: Licensee Oa Publishing London 2012 Creative Commons Attribumentioning

confidence: 99%

Pharmaceutical applications of bioactive peptides

Danquah¹,

Agyei²

2012

OA Biotechnology

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IntroductionThere is a mounting interest in the therapeutic potential of bioactive peptides which collectively present a cornucopia of bioactivities for exploitation in vivo. Bioactive peptides trigger certain functionalities such as antioxidative, antimicrobial, antihypertensive, cytomodulatory and immunomodulatory activities in the living body system. With research and development, there exists an opportunity to effectively harness these functionalities for the treatment, prevention and mitigation of different medical conditions. This critical review discusses some potential therapeutic applications of bioactive peptides in the light of advances in general biopharmaceutical production based on proteomics and genomics. ConclusionBioactive peptides are ubiquitous biomolecules widely abundant and easily obtained from food proteins and there is no limit to how many can be obtained froma single food protein.

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Concentration and selective fractionation of an antihypertensive peptide from an alfalfa white proteins hydrolysate by mixed ion-exchange centrifugal partition chromatography

Cited by 18 publications

References 22 publications

Bioactive Peptides from Cartilage Protein Hydrolysate of Spotless Smoothhound and Their Antioxidant Activity In Vitro

Bioactive Peptides from Cartilage Protein Hydrolysate of Spotless Smoothhound and Their Antioxidant Activity In Vitro

A Review of the Latest Advances in Encrypted Bioactive Peptides from Protein-Rich Waste

Pharmaceutical applications of bioactive peptides

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