Concentration and composition of the lipoprotein classes in human umbilical cord serum

Winkler, L; Schlag, B; Goetze, E

doi:10.1016/0009-8981(77)90095-x

Cited by 36 publications

(11 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Only 0.5% of the samples had both elevated cholesterol and TG. These cholesterol and TG distributions are similar to those previously reported (5,7,10,(28)(29)(30)(31)(32)(33)(34).…”

Section: Resultssupporting

confidence: 91%

High-Density Lipoprotein Subclass Distribution And Human Cord Blood Lipid Levels

1986

View full text Add to dashboard Cite

ABSTRACT. The high-density lipoprotein (HDL) subclass distribution was examined by gradient gel electrophoresis (gge) in 154 human cord blood samples, and determinations of triglyceride, total cholesterol, and HDL-cholesterol levels were performed. Three distinct gge patterns were recognizable. The first pattern, termed the normal (gge) pattern, was distinguished by a prominent double peak in the (HDL2,),, region and a pronounced peak in the (HDL3b)gge region. Minor peaks, or shoulders, were also seen in the (HDLZb)gge and (HDL3Jae regions, and a valley was present in the (HDL3,),,, region. This pattern was associated with normal lipid levels for cord blood plasma (mean triglycerides: 30-42 mg/dl; mean total cholesterol 62-85 mg/dl; mean HDL-cholesterol: 34-41 mg/ dl).The second pattern, termed the 2b(gge) pattern, contained a major peak in the ( H D L z~)~~, region rather than the shoulder seen in the normal (gge) pattern, while the (HDL2.)gge, (HDL3b),e, and (HDL3,),, regions were less pronounced. This pattern was associated with elevated total cholesterol and HDL-C levels (means 85-102 and 49-56 mg/dl, respectively). The third pattern, termed the 3b(gge) pattern, was characterized by a paucity of material in the (HDL2,,),, region, a single peak in the (HDLza),, region, and either a relative increase in the (HDL3b),e region, or a simultaneous increase in both (HDLs~),~ and (HDL3e),e. This pattern was associated with elevated triglyceride levels (means 78-88 mg/dl) and decreased HDL-C levels (means 20-30 mg/dl). Only two infants had a simultaneous elevation of triglycerides and total cholesterol and both cases exhibited the 3b(gge) pattern. Our study demonstrates that although the triglyceride and cholesterol levels in the newborn are much lower than those in adults, they are the important factors associated with the HDL subclass distribution. Elevated cholesterol was related to increased particles in the (HDL2b), region while elevated triglyceride levels were associated with a decrease in (HDL2b+2a)gge particles and a concomitant increase in (HDLsb),, particles. (Pediatr Res 20: 487-491, 1986) Abbreviations HDL, high-density lipoproteins TG, triglyceride TC, total cholesterol HDL-C, HDL-cholesterol gge, gradient gel electrophoresis CB, cord blood Received October 7, 1985: accepted January 23, 1986. Address correspondence and requests for reprints to Orsolya Genzel-Boroviczeny, M.D., Donner Laboratory, 1-213, University of California, Berkeley, CA 94720.This work was supported by NIH Program Project Grant HL 18574 from the National Heart, Lung, and Blood Institute of the National Institutes of Health and NIH National Research Service Award Training Grant HL 07279.There is growing evidence that HDL, and in particular the HDL2 subclass, have a protective role in the process of atherogenesis (1-3). In contrast to adults, in whom low density lipoproteins predominate, HDL is the predominant lipoprotein species in human CB (4-10). Davis et al. (4) found that in healthy term infants with lipoprotein levels below 10...

show abstract

“…Only 0.5% of the samples had both elevated cholesterol and TG. These cholesterol and TG distributions are similar to those previously reported (5,7,10,(28)(29)(30)(31)(32)(33)(34).…”

Section: Resultssupporting

confidence: 91%

High-Density Lipoprotein Subclass Distribution And Human Cord Blood Lipid Levels

1986

View full text Add to dashboard Cite

show abstract

“…21 While the IDL and LDL are decreased in the neonate, the decrease is not merely an overall decrease but also a shift, as the mass of the neonatal IDL represents only 8% to 9% of the total d = 1.006 to 1.063 mass, while in the adult, the IDL represents 11% to 13% of the total LDL. The total d = 1.006 to 1.063 g/ml concentration of 80 mg/100 ml reported here is in general agreement with that reported by several investigators, 1 " 8 but is higher than the approximately 60 mg/100 ml reported by both Winkler et al 9 and Wille and Phillips. 10 The reasons for this discrepancy between our values along with the values of other workers when compared to the values reported by Winkler et al and Wille and Phillips are unknown, but the population studied and the methodology employed may be factors.…”

Section: Discussionsupporting

confidence: 93%

Neonatal umbilical cord blood lipoproteins. Isolation and characterization of intermediate density and low density lipoproteins.

Davis¹,

Forte²

1982

Arteriosclerosis

View full text Add to dashboard Cite

show abstract

“…6 " 19 ' 27 The mean total HDL cholesterol level of 24.9 mg/dl and HDL triglyceride level of 5 mg/dl in the present study is commensurate with these reduced levels and is on the low end of the range of values that have been reported by others (i.e., 24 to 39 mg/ dl HDL cholesterol and 5 to 13 mg/dl HDL triglyceride). Our lower values may reflect either differences in the techniques used to isolate the HDL or differences among the populations studied.…”

Section: Discussionsupporting

confidence: 80%

Umbilical cord blood lipoproteins. Isolation and characterization of high density lipoproteins.

Davis¹,

Forte²,

Nichols³

et al. 1983

Arteriosclerosis

View full text Add to dashboard Cite

High density lipoproteins (d = 1.063 to 1.21 g/ml) were isolated from both individual and pooled umbilical cord blood plasma by sequential preparative ultracentrifugation. Mean total HDL concentration was 139 ± 9.1 mg/dl, with mean values for cholesterol and triglyceride of 24.9 mg/dl and 5.0 mg/dl, respectively. The particle size distribution of HDL particles was examined by gradient gel electrophoresis (GGE) on 4-30% gels; the range of particle sizes was broader than that reported for adult HDL and showed a deficiency of material in the region defined as (HDL 3a ). ge . In contrast, the latter is a major peak in adult HDL. The total HDL isolated from the pooled samples were subfractionated by density gradient ultracentrifugation. The subfractions, as well as the total HDL used as starting material, were analyzed for size (both GGE and electron microscopy), chemical composition, and apolipoprotein content by both sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis and radioimmunoassay for apo E. Apo E concentration of 1.76 mg/dl in cord blood HDL is high relative to the low HDL cholesterol value of 24.9 mg/dl. In addition to increased amounts of apo E, cord blood HDL contained apo A-l, apo A-ll, and traces of apo C's. The less dense HDL (d =£ 1.115) were large (12.0 nm), were enriched in unesterified cholesterol, and contained the bulk of the apo E which was present as a reducible mixed disulf ide complex (E-A-ll). The more dense HDL (d 3= 1.115) were smaller and were enriched in total protein and cholesteryl ester. The unusual HDL size distribution, as determined by GGE, and the elevated apo E and apo E-A-ll complex content of cord blood HDL point to an altered metabolism of lipoproteins with densities of 1.063 to 1.21 g/ml in the fetus. (Arteriosclerosis 3:357-365, July/August 1983) T he study of high density lipoproteins (HDL), their metabolism, and the factors that control that metabolism has recently gained added impetus as the evidence for a protective role for HDL in atherosclerosis has grown. Received December 20, 1982; revision accepted February 24, 1983. process is thought to begin in childhood, the study of the neonatal lipoprotein patterns may allow the identification of lipoprotein metabolic abnormalities and thus allow early intervention in the disease process. 4 Additionally, characterization of the neonatal lipoproteins and their metabolism may give added insights into the control mechanisms and pathways that produce the spectrum of particles seen in adult HDL and adult lipoproteins in general.Several investigators have quantitated the cholesterol and triglyceride level in cord blood HDL. 5" 17 The results have been somewhat variable but show that approximately 50% of the total cord blood cholesterol is carried in HDL, in marked contrast to the adult where the majority of the cholesterol is carried in the LDL. Although triglyceride, cholesterol, and apolipoprotein A-l levels in cord blood have been investigated, little is known about cord blood HDL and the few studies of isolated...

show abstract

Concentration and composition of the lipoprotein classes in human umbilical cord serum

Cited by 36 publications

References 17 publications

High-Density Lipoprotein Subclass Distribution And Human Cord Blood Lipid Levels

High-Density Lipoprotein Subclass Distribution And Human Cord Blood Lipid Levels

Neonatal umbilical cord blood lipoproteins. Isolation and characterization of intermediate density and low density lipoproteins.

Umbilical cord blood lipoproteins. Isolation and characterization of high density lipoproteins.

Contact Info

Product

Resources

About