Umbilical cord blood lipoproteins. Isolation and characterization of high density lipoproteins.

Davis, Paul A.; Forte, Trudy M.; Nichols, Alex V.; Blum, Conrad B.

doi:10.1161/01.atv.3.4.357

Cited by 32 publications

(22 citation statements)

References 37 publications

(22 reference statements)

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“…This pattern agrees well with that previously described by Davis et al (4). Of our "control" group 89%, i.e.…”

Section: Discussionsupporting

confidence: 94%

“…Although not shown in Figure 2, the shoulder in the (HDL3,), region frequently resolves into two small components. We have termed this pattern the normal (gge) pattern and it is similar to that previously described for pooled normal CB samples (4). The second pattern is shown in Figure 2B and contains a major peak in the (HDL2,), region rather than the shoulder seen in the normal pattern.…”

Section: Resultssupporting

confidence: 60%

“…Davis et al (4), using nondenaturing gradient gel electrophoresis, demonstrated that pooled and individual CB samples from full-term normal infants had a very characteristic size distribution profile which was different from that of adults. They observed that cord blood HDL possessed larger-sized particles in the (HDL2b)gge region, a double peak in the (HDLZa)gge region, and smaller HDL in the (HDL,,), region; additionally, there was little or no HDL in the (HDL3,), region.…”

Section: Discussionmentioning

confidence: 99%

“…In contrast to adults, in whom low density lipoproteins predominate, HDL is the predominant lipoprotein species in human CB (4-10). Davis et al (4) found that in healthy term infants with lipoprotein levels below 100 mg/dl, cord blood HDL differed from that of adult in size distribution and in apolipoprotein content. They noted that the less dense HDL components of CB were larger, enriched in unesterified cholesterol, and contained the bulk of apolipoprotein E; the latter was principally in the apolipoprotein (E-AII) complex form.…”

mentioning

confidence: 99%

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High-Density Lipoprotein Subclass Distribution And Human Cord Blood Lipid Levels

1986

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ABSTRACT. The high-density lipoprotein (HDL) subclass distribution was examined by gradient gel electrophoresis (gge) in 154 human cord blood samples, and determinations of triglyceride, total cholesterol, and HDL-cholesterol levels were performed. Three distinct gge patterns were recognizable. The first pattern, termed the normal (gge) pattern, was distinguished by a prominent double peak in the (HDL2,),, region and a pronounced peak in the (HDL3b)gge region. Minor peaks, or shoulders, were also seen in the (HDLZb)gge and (HDL3Jae regions, and a valley was present in the (HDL3,),,, region. This pattern was associated with normal lipid levels for cord blood plasma (mean triglycerides: 30-42 mg/dl; mean total cholesterol 62-85 mg/dl; mean HDL-cholesterol: 34-41 mg/ dl).The second pattern, termed the 2b(gge) pattern, contained a major peak in the ( H D L z~)~~, region rather than the shoulder seen in the normal (gge) pattern, while the (HDL2.)gge, (HDL3b),e, and (HDL3,),, regions were less pronounced. This pattern was associated with elevated total cholesterol and HDL-C levels (means 85-102 and 49-56 mg/dl, respectively). The third pattern, termed the 3b(gge) pattern, was characterized by a paucity of material in the (HDL2,,),, region, a single peak in the (HDLza),, region, and either a relative increase in the (HDL3b),e region, or a simultaneous increase in both (HDLs~),~ and (HDL3e),e. This pattern was associated with elevated triglyceride levels (means 78-88 mg/dl) and decreased HDL-C levels (means 20-30 mg/dl). Only two infants had a simultaneous elevation of triglycerides and total cholesterol and both cases exhibited the 3b(gge) pattern. Our study demonstrates that although the triglyceride and cholesterol levels in the newborn are much lower than those in adults, they are the important factors associated with the HDL subclass distribution. Elevated cholesterol was related to increased particles in the (HDL2b), region while elevated triglyceride levels were associated with a decrease in (HDL2b+2a)gge particles and a concomitant increase in (HDLsb),, particles. (Pediatr Res 20: 487-491, 1986) Abbreviations HDL, high-density lipoproteins TG, triglyceride TC, total cholesterol HDL-C, HDL-cholesterol gge, gradient gel electrophoresis CB, cord blood Received October 7, 1985: accepted January 23, 1986. Address correspondence and requests for reprints to Orsolya Genzel-Boroviczeny, M.D., Donner Laboratory, 1-213, University of California, Berkeley, CA 94720.This work was supported by NIH Program Project Grant HL 18574 from the National Heart, Lung, and Blood Institute of the National Institutes of Health and NIH National Research Service Award Training Grant HL 07279.There is growing evidence that HDL, and in particular the HDL2 subclass, have a protective role in the process of atherogenesis (1-3). In contrast to adults, in whom low density lipoproteins predominate, HDL is the predominant lipoprotein species in human CB (4-10). Davis et al. (4) found that in healthy term infants with lipoprotein levels below 10...

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“…This pattern agrees well with that previously described by Davis et al (4). Of our "control" group 89%, i.e.…”

Section: Discussionsupporting

confidence: 94%

Section: Resultssupporting

confidence: 60%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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High-Density Lipoprotein Subclass Distribution And Human Cord Blood Lipid Levels

1986

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“…33 All these conditions differ from the A-I M + by the presence of larger (HDLaJgg, particles and/or even smaller (HDLjcJgg. particles.…”

Section: Discussionmentioning

confidence: 99%

Apolipoprotein A-IMilano. Correlation between high density lipoprotein subclass distribution and triglyceridemia.

Franceschini¹,

Calabresi²,

Tosi³

et al. 1987

Arteriosclerosis

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Carriers of the apolipoprotein A-I unino (apo A-I M ) variant represent a selected group of subjects showing low levels of high density lipoprotein (HDL), variable hypertrlglycerldemia, and low prevalence of atherosclerotic vascular disease. The distribution of HDL subtractions and the correlation with abnormalities in triglycerlde transport were determined In these subjects. Sera from 24 apo A-I M carriers (A-I H + and from age-and sex-matched normollpldemlc controls (A-I u~) were analyzed by rate zonal ultracentrlrugatlon. The A-I H + subjects showed a marked decrease of HDL 3 mass with reduced flotation rates and major compositional alterations; the HDL 2 were nearly absent The HDL subclasses from 10 A-I u + subjects were resolved according to particle size by gradient gel eiectrophoresis (GGE). The HDL patterns detected In the carriers were unique In exhibiting a distinct peak In the (HDLuJgg, interval, undetectable In the controls. Three patterns reflecting the relative contributions of smaller (HDLjJgg. and larger (HDLj.)™ particles could be distinguished In the carriers, and these were clearly related to different triglycerlde and HDL cholesterol levels in plasma. These findings In a highly selected group of subjects with generally low HDL levels and quite variable triglyceridemia confirmed the existence of relationships between alterations In triglycerlde transport and abnormalities In the HDL subclass distribution, possibly reflecting the variable atherosclerotic risk in hypertrlglycerldemlc subjects. (Arteriosclerosis 7:426-435, July/August 1987)

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A Large High-Density Lipoprotein Enriched in Apolipoprotein C-I

Kwiterovich¹,

Cockrill²,

Virgil³

et al. 2005

JAMA

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OW BIRTH WEIGHT IS ASSOCIated with cardiovascular risk factors and death in adulthood. 1 Differences in the size, molecular weight, composition, and quantity of lipoprotein subclasses are associated with coronary artery disease (CAD). 2 In our initial study of lipoprotein heterogeneity in cord blood, infants born small for gestational age (SGA) had higher levels of triglyceriderich very low-density lipoprotein (VLDL) and intermediate low-density lipoprotein (IDL) than infants born appropriate for gestational age. 3 This finding extended the observations of others in SGA infants 4-7 and suggested a link between higher triglyceride-rich VLDL subclasses in SGA infants and future CAD. We assessed a large high-density lipoprotein (HDL) subclass in cord blood 8-15 enriched in apolipoprotein C-I (apo C-I). Apolipoprotein C-I is a 6.6-kDa apolipoprotein, and in adults it is a component of VLDL, IDL, and HDL. 16 Apolipoprotein C-I displaces apo E from VLDL and IDL, thereby decreasing their clearance from plasma. 17 Apolipoprotein C-I decreases the binding

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Umbilical cord blood lipoproteins. Isolation and characterization of high density lipoproteins.

Cited by 32 publications

References 37 publications

High-Density Lipoprotein Subclass Distribution And Human Cord Blood Lipid Levels

High-Density Lipoprotein Subclass Distribution And Human Cord Blood Lipid Levels

Apolipoprotein A-IMilano. Correlation between high density lipoprotein subclass distribution and triglyceridemia.

A Large High-Density Lipoprotein Enriched in Apolipoprotein C-I

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