Neonatal umbilical cord blood lipoproteins. Isolation and characterization of intermediate density and low density lipoproteins.

Davis, Paul A.; Forte, Trudy M.

doi:10.1161/01.atv.2.1.37

Cited by 15 publications

(15 citation statements)

References 28 publications

(25 reference statements)

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“…The relative cholesterol load is far worse in the breast-fed human infant, which acquires ?18 mg/kg each day of cholesterol from its mother's milk under circumstances in which it is synthesizing only ?25 mg/kg (62,63). This massive load of exogenous cholesterol relative to the rate of endogenous synthesis in the infant is manifest by the inhibition of endogenous cholesterol synthesis, a nearly 3-fold increase in the circulating LDL-cholesterol concentration, and, in the infant with NPC disease, prolonged cholestasis (62,64). Given the relationship between dietary cholesterol intake and liver disease in this lysosomal storage disorder, it would seem prudent to avoid breastfeeding and other sources of dietary cholesterol and to begin the administration of an agent such as ezetimibe to the infant as soon as the diagnosis is made.…”

Section: Discussionmentioning

confidence: 99%

Lysosomal unesterified cholesterol content correlates with liver cell death in murine Niemann-Pick type C disease

Beltroy

Liu

Dietschy

et al. 2007

Journal of Lipid Research

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Section: Discussionmentioning

confidence: 99%

Lysosomal unesterified cholesterol content correlates with liver cell death in murine Niemann-Pick type C disease

Beltroy

Liu

Dietschy

et al. 2007

Journal of Lipid Research

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“…5 . Typically, during fetal development both the TC and LDL-C levels decrease in late gestation (62)(63)(64)(65). In the lamb, for example, the TC declines from ‫ف‬ 40 mg/dl to ‫ف‬ 25 mg/dl and the LDL-C decreases from ‫ف‬ 25 mg/dl to only ‫ف‬ 10 mg/dl over the course of fetal development (62).…”

Section: Concentration Of Cholesterol In the Plasma And Disorders Of mentioning

confidence: 99%

Thematic review series: Brain Lipids. Cholesterol metabolism in the central nervous system during early development and in the mature animal

Dietschy

Turley

2004

Journal of Lipid Research

920

885

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Unesterified cholesterol is an essential structural component of the plasma membrane of every cell. During evolution, this membrane came to play an additional, highly specialized role in the central nervous system (CNS) as the major architectural component of compact myelin. As a consequence, in the human the mean concentration of unesterified cholesterol in the CNS is higher than in any other tissue ( ‫ف‬ 23 mg/g). Furthermore, even though the CNS accounts for only 2.1% of body weight, it contains 23% of the sterol present in the whole body pool. In all animals, most growth and differentiation of the CNS occurs in the first few weeks or years after birth, and the cholesterol required for this growth apparently comes exclusively from de novo synthesis. Currently, there is no evidence for the net transfer of sterol from the blood into the brain or spinal cord. In adults, the rate of synthesis exceeds the need for new structural sterol, so that net movement of cholesterol out of the CNS must take place. At least two pathways are used for this excretory process, one of which involves the formation of 24( S )-hydroxycholesterol. Whether or not changes in the plasma cholesterol concentration alter sterol metabolism in the CNS or whether such changes affect cognitive function in the brain or the incidence of dementia remain uncertain at this time.

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“…Plasmas were processed and pooled (10 to 20 samples per pool) essentially as described previously. 19 In brief, this entailed the quantitation of the levels of triglyceride and total cholesterol in individual samples, the exclusion of cord blood plasma with levels of either triglyceride or cholesterol exceeding 100 mg/dl, and the pooling of plasma without regard to sex. Samples were pooled since we previously showed no gender-related differences between cord blood lipoproteins when individual cord blood samples were analyzed.…”

Section: Llpoproteln Isolationmentioning

confidence: 99%

“…Samples were pooled since we previously showed no gender-related differences between cord blood lipoproteins when individual cord blood samples were analyzed. 19 The total HDL were isolated from the cord blood plasma by preparative ultracentrifugation according to the method of Lindgren et al, 20 except that all ultracentrifugation steps were carried out at 4°C. The HDL was fractionated into HDL, and HDLj.…”

Section: Llpoproteln Isolationmentioning

confidence: 99%

See 1 more Smart Citation

Umbilical cord blood lipoproteins. Isolation and characterization of high density lipoproteins.

Davis¹,

Forte²,

Nichols³

et al. 1983

Arteriosclerosis

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High density lipoproteins (d = 1.063 to 1.21 g/ml) were isolated from both individual and pooled umbilical cord blood plasma by sequential preparative ultracentrifugation. Mean total HDL concentration was 139 ± 9.1 mg/dl, with mean values for cholesterol and triglyceride of 24.9 mg/dl and 5.0 mg/dl, respectively. The particle size distribution of HDL particles was examined by gradient gel electrophoresis (GGE) on 4-30% gels; the range of particle sizes was broader than that reported for adult HDL and showed a deficiency of material in the region defined as (HDL 3a ). ge . In contrast, the latter is a major peak in adult HDL. The total HDL isolated from the pooled samples were subfractionated by density gradient ultracentrifugation. The subfractions, as well as the total HDL used as starting material, were analyzed for size (both GGE and electron microscopy), chemical composition, and apolipoprotein content by both sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis and radioimmunoassay for apo E. Apo E concentration of 1.76 mg/dl in cord blood HDL is high relative to the low HDL cholesterol value of 24.9 mg/dl. In addition to increased amounts of apo E, cord blood HDL contained apo A-l, apo A-ll, and traces of apo C's. The less dense HDL (d =£ 1.115) were large (12.0 nm), were enriched in unesterified cholesterol, and contained the bulk of the apo E which was present as a reducible mixed disulf ide complex (E-A-ll). The more dense HDL (d 3= 1.115) were smaller and were enriched in total protein and cholesteryl ester. The unusual HDL size distribution, as determined by GGE, and the elevated apo E and apo E-A-ll complex content of cord blood HDL point to an altered metabolism of lipoproteins with densities of 1.063 to 1.21 g/ml in the fetus. (Arteriosclerosis 3:357-365, July/August 1983) T he study of high density lipoproteins (HDL), their metabolism, and the factors that control that metabolism has recently gained added impetus as the evidence for a protective role for HDL in atherosclerosis has grown. Received December 20, 1982; revision accepted February 24, 1983. process is thought to begin in childhood, the study of the neonatal lipoprotein patterns may allow the identification of lipoprotein metabolic abnormalities and thus allow early intervention in the disease process. 4 Additionally, characterization of the neonatal lipoproteins and their metabolism may give added insights into the control mechanisms and pathways that produce the spectrum of particles seen in adult HDL and adult lipoproteins in general.Several investigators have quantitated the cholesterol and triglyceride level in cord blood HDL. 5" 17 The results have been somewhat variable but show that approximately 50% of the total cord blood cholesterol is carried in HDL, in marked contrast to the adult where the majority of the cholesterol is carried in the LDL. Although triglyceride, cholesterol, and apolipoprotein A-l levels in cord blood have been investigated, little is known about cord blood HDL and the few studies of isolated...

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Neonatal umbilical cord blood lipoproteins. Isolation and characterization of intermediate density and low density lipoproteins.

Abstract: Intermediate density lipoproteins (IDL)(

Cited by 15 publications

References 28 publications

Lysosomal unesterified cholesterol content correlates with liver cell death in murine Niemann-Pick type C disease

Lysosomal unesterified cholesterol content correlates with liver cell death in murine Niemann-Pick type C disease

Thematic review series: Brain Lipids. Cholesterol metabolism in the central nervous system during early development and in the mature animal

Umbilical cord blood lipoproteins. Isolation and characterization of high density lipoproteins.

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