Computational Investigation of Interaction between Nanoparticles and Membranes: Hydrophobic/Hydrophilic Effect

Yang, Li; Chen, Xin; Gu, Ning

doi:10.1021/jp8051906

Cited by 254 publications

(191 citation statements)

References 39 publications

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“…Thus, it seems that the NP-SLB interaction is mainly driven by the high surface energy of the bare NPs that tend to extract lipids from the bilayer. 16,57 Montis and co-workers 58 Although the HC constitutes the most permanent protein shell on the NP surface and it is considered the biological identity of the NP, a soft corona is also associated in vivo to the NPs and its role in the bio-nano-interaction is still elusive. For this reason, experiments with pure serum and NPs in serum were performed, to investigate if weakly associated and free proteins have a role in the NP-SLB interaction.…”

Section: Discussionmentioning

confidence: 99%

“…5 NPs can adhere to the lipid bilayer and cause changes in the lipid phase, 7 induce formation of lipid domains [8][9] or pores and extract lipids 10 inducing lipid bilayer disruption. [11][12] Physical chemical properties of NPs, 5,13 such as size, 4,11,[14][15] charge 12,16 and surface chemistry [17][18][19][20] are the main factors modulating NP-membrane interactions.…”

Section: Introductionmentioning

confidence: 99%

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The effect of the protein corona on the interaction between nanoparticles and lipid bilayers

Silvio

Maccarini

Parker

et al. 2017

Journal of Colloid and Interface Science

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2 HypothesisIt is known that nanoparticles (NPs) in a biological fluid are immediately coated by a protein corona (PC), composed of a hard (strongly bounded) and a soft (loosely associated) layers, which represents the real nano-interface interacting with the cellular membrane in vivo. In this regard, supported lipid bilayers (SLB) have extensively been used as relevant model systems for elucidating the interaction between biomembranes and NPs. Herein we show how the presence of a PC on the NP surface changes the interaction between NPs and lipid bilayers with particular care on the effects induced by the NPs on the bilayer structure. ExperimentsIn the present work we combined Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D) and Neutron Reflectometry (NR) experimental techniques to elucidate how the NPmembrane interaction is modulated by the presence of proteins in the environment and their effect on the lipid bilayer. FindingsOur study showed that the NP-membrane interaction is significantly affected by the presence of proteins and in particular we observed an important role of the soft corona in this phenomenon. KEYWORDSsupported lipid bilayer, protein corona nanoparticles, quartz crystal microbalance, neutron reflectometry, soft corona, hard corona. ABBREVIATIONNPs nanoparticles; SLB supported lipid bilayer; PC protein corona; QCM-D quartz crystal microbalance with dissipation; NR neutron reflectometry; PS polystyrene; PBS phosphate buffered saline; FBS fetal bovine serum; HC hard corona; SC soft corona; DOPC 1,2-Dioleoylsn-glycero-3-phosphocholine; SLD scattering length density; APM area per molecule; 4MW 4 matching water; SMW silicon matching water; LUV large unilamellar vesicle; GUV giant unilamellar vesicle; DPPC Dipalmitoyl-phosphatidyl-choline.3

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The effect of the protein corona on the interaction between nanoparticles and lipid bilayers

Silvio

Maccarini

Parker

et al. 2017

Journal of Colloid and Interface Science

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“…The calculation base on the free energy in this study showed that the size of nanoparticles affects the translocation time differently, suggesting that the size has significant impacts on its translocation across the lipid bilayer [40]. Results of coarse-grained molecular dynamics simulations indicated that a hydrophobic nanoparticle can result in the inclusion into the DPPC bilayer, whereas a semihydrophilic nanoparticle is only found to adsorb into the membrane because of the potential substantial energy barrier of particle wrapping, implicating that the endocytosis-like mechanism is an energy-mediated process [41]. Thermodynamics of charged nanoparticle adsorption on charge-neutral membranes was also simulated [42].…”

Section: Cellular Uptake and Processingmentioning

confidence: 99%

In vitro biological effects of magnetic nanoparticles

Chen

2012

Chin. Sci. Bull.

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Magnetic nanoparticles (MNPs) have great potential for a wide use in various biomedical applications due to their unusual properties. It is critical for many applications that the biological effects of nanoparticles are studied in depth. To date, many disparate results can be found in the literature regarding nanoparticle-biological factors interactions. This review highlights recent developments in this field with particular focuses on in vitro MNPs-cell interactions. The effect of MNPs properties on cellular uptake and cytotoxicity evaluation of MNPs were discussed. Some employed methods are also included. Moreover, nanoparticle-cell interactions are mediated by the presence of proteins absorbed from biological fluids on the nanoparticle. Many questions remain on the effect of nanoparticle surface (in addition to nanoparticle size) on protein adsorption. We review papers related to this point too. The current development of magnetic nanoparticles (MNPs) requires a better understanding of its biological effects. A great deal of work has been conducted to study MNPsbiological factors interactions. Many researchers bend themselves to in vitro cell-based assessment. In this work, the current knowledge of how the in vitro cultured cells can interact with the exposed colloid MNPs is discussed as well as the effect of nanoparticle size and surface properties on cell responses. The specific cell line selected for in vitro assay is intended to model a response or phenomenon likely observed or sensitized by particles in vivo. Here, the frequent lack of consistency or predictability between in vitro models and in vivo observations, which is because of the different biological conditions particles exposed to and the changed cell phenotype against primary cell types, is out of our consideration. As we have known, cell monocultures as measured by in vitro assays rarely react in such isolated pathways in native tissues comprosed of multiple, dynamically communicative cell types that produce non-linear and correlated response to foreign materials.Adsorption of proteins onto the nanoparticle surface happens immediately after particles come in contact with a biological fluid, and it is the proteins associate with nanoparticles, leading to a protein "corona" which defines the biological identity of the particle. Here, we also try to review some current work on associated protein "corona" when nanoparticles were incubated in biological medium.

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“…Both experimental studies and computer simulations indicated that cationic AuNMs induce more disruption of the cell membrane than anionic AuNMs [68,69], as it depends on the charge density. For the hydrophobic interactions, a simulation study showed that hydrophobic NMs (diameter: 10 nm) could be embedded into the cell membrane, whereas semihydrophilic NMs only adhered to the membrane [70]. 11mer capto1undecanesulfonate AuNPs that only had hydrophilic sulfonate ligands were endocy tosed by cells, whereas the 66-34 1octanethiol AuNPs (AuNPs coated with a 2:1 molar mix ture of 11mercapto1undecanesulfonate and 1octanethiol) with a hydrophilic-hydrophobic striated ligand shell directly penetrated the cell membrane owing to ligand rearrangement [57].…”

Section: Future Science Groupmentioning

confidence: 99%

“…For example, Gu et al simulated the effect of the hydro phobic property of NMs and the effect of charges on NM-biomembrane systems [70,91]. Hydrophobic interaction and electrostatic attraction improved the interaction process.…”

Section: Mathematical and Numerical Modeling Approaches For Nm-biomembrmentioning

confidence: 99%

Advances in The Understanding of Nanomaterial–Biomembrane Interactions and Their Mathematical and Numerical Modeling

Lin

et al. 2013

Nanomedicine

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The widespread application of nanomaterials (NMs), which has accompanied advances in nanotechnology, has increased their chances of entering an organism, for example, via the respiratory system, skin absorption or intravenous injection. Although accumulating experimental evidence has indicated the important role of NM–biomembrane interaction in these processes, the underlying mechanisms remain unclear. Computational techniques, as an alternative to experimental efforts, are effective tools to simulate complicated biological behaviors. Computer simulations can investigate NM–biomembrane interactions at the nanoscale, providing fundamental insights into dynamic processes that are challenging to experimental observation. This paper reviews the current understanding of NM–biomembrane interactions, and existing mathematical and numerical modeling methods. We highlight the advantages and limitations of each method, and also discuss the future perspectives in this field. Better understanding of NM–biomembrane interactions can benefit various fields, including nanomedicine and diagnosis.

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Computational Investigation of Interaction between Nanoparticles and Membranes: Hydrophobic/Hydrophilic Effect

Cited by 254 publications

References 39 publications

The effect of the protein corona on the interaction between nanoparticles and lipid bilayers

The effect of the protein corona on the interaction between nanoparticles and lipid bilayers

In vitro biological effects of magnetic nanoparticles

Advances in The Understanding of Nanomaterial–Biomembrane Interactions and Their Mathematical and Numerical Modeling

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