Compositional effects on the formation of a calcium phosphate layer and the response of osteoblast-like cells on polymer-bioactive glass composites

Lu, Helen H.; Tang, Amy; Oh, Se Baek; Spalazzi, Jeffrey P.; Dionisio, Kathie

doi:10.1016/j.biomaterials.2005.04.005

Cited by 119 publications

(98 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The increase of calcium and phosphorus in this study is in accordance with other investigations that have reported calcium and phosphorus deposition on polymer and hydroxyapatite composite following12 hours of SBF immersion [15]. Bioactive glass and polymer composition are essential in the increase of Ca and P percentages [16].…”

Section: Discussionsupporting

confidence: 92%

Composition of chitosan-hydroxyapatite-collagen composite scaffold evaluation after simulated body fluid immersion as reconstruction material

Verisqa

Triaminingsih

Corputty

2017

J. Phys.: Conf. Ser.

View full text Add to dashboard Cite

Abstract. Hydroxyapatite (HA) formation is one of the most important aspects of bone regeneration. Because domestically made chitosan-hydroxyapatite-collagen composite scaffolding from crab shell and bovine bone and tendon has potential as a maxillofacial reconstruction material, the material's HA-forming ability requires evaluation. The aim of this research is to investigate chitosan-hydroxyapatite-collagen composite scaffold's potential as a maxillofacial reconstruction material by observing the scaffold's compositional changes. Scaffold specimens were immersed in 37°C simulated body fluid (SBF) for periods of 2, 4, 6, and 8 days. Scaffold composition was then evaluated by using energy dispersive spectroscopy (EDS). The calcium (Ca) and phosphorus (P) percentages of the scaffold were found to increase following SBF immersion. The high Ca/P ratio (3.82) on the scaffold indicated HA formation. Ion exchange played a significant role in the increased percentages of Ca and P, which led to new HA layer formation. The scaffold's HA acted as a nucleation site of Ca and P from the SBF, with collagen and chitosan as the scaffold's matrix. Chitosan-hydroxyapatitecollagen composite scaffold shows potential as a maxillofacial reconstruction material, since its composition favors HA formation.

show abstract

Section: Discussionsupporting

confidence: 92%

Composition of chitosan-hydroxyapatite-collagen composite scaffold evaluation after simulated body fluid immersion as reconstruction material

Verisqa

Triaminingsih

Corputty

2017

J. Phys.: Conf. Ser.

View full text Add to dashboard Cite

show abstract

“…Bioglass is a highly biocompatible material which can induce osteogenesis in simulated body fluids 20) . It has been suggested as an adjunct material to the treatment of tooth cracks or fractures because of its strong chemical bonding with hydroxyapatite 21,22) .…”

Section: Discussionmentioning

confidence: 99%

In Vitro Study of Dentinal Tubule Occlusion with Sol-gel DP-bioglass for Treatment of Dentin Hypersensitivity

et al. 2007

View full text Add to dashboard Cite

DP-bioglass paste has been demonstrated to produce 60 μm of sealing depth on exposed dentinal tubules. However, the occlusive effect depended on a continuous placement of DP-bioglass paste on dentinal surface for three days. In a bid to fabricate highly reactive DP-bioglass particles, a sol-gel method was used together with HNO3, NaOH, and H3PO4 as catalysts. As a result, the application time of DP-bioglass paste was significantly reduced to 10 minutes. Percentage of tubular occlusion with DP-bioglass was 53.2-65.4％, while One Coat Bond and Seal & Protect yielded 51.3％ and 41.2％ respectively. Further, the average depth of tubular occlusion with DP-bioglass was 55.8-62.7 μm, while One Coat Bond and Seal & Protect produced 40.8 μm and 32.5 μm respectively. In conclusion, the best sealing performance of tubular occlusion was rendered by DP-bioglass catalyzed with HNO3. Its performance was significantly better than Seal & Protect, and was considered to exhibit the greatest potential in treating dentin hypersensitivity.

show abstract

“…42 Additionally, media calcium concentrations (n = 5) were quantified using the Arsenazo III dye (Pointe Scientific), with absorbance measured at 620 nm using a microplate reader. 43 …”

Section: Mineralizationmentioning

confidence: 99%

A Hydrogel-Mineral Composite Scaffold for Osteochondral Interface Tissue Engineering

Khanarian

Jiang

Wan

et al. 2012

Tissue Engineering Part A

109

View full text Add to dashboard Cite

Osteoarthritis is the leading cause of physical disability among Americans, and tissue engineered cartilage grafts have emerged as a promising treatment option for this debilitating condition. Currently, the formation of a stable interface between the cartilage graft and subchondral bone remains a significant challenge. This study evaluates the potential of a hybrid scaffold of hydroxyapatite (HA) and alginate hydrogel for the regeneration of the osteochondral interface. Specifically, the effects of HA on the response of chondrocytes were determined, focusing on changes in matrix production and mineralization, as well as scaffold mechanical properties over time. Additionally, the optimal chondrocyte population for interface tissue engineering was evaluated. It was observed that the HA phase of the composite scaffold promoted the formation of a proteoglycan-and type II collagen-rich matrix when seeded with deep zone chondrocytes. More importantly, the elevated biosynthesis translated into significant increases in both compressive and shear moduli relative to the mineral-free control. Presence of HA also promoted chondrocyte hypertrophy and type X collagen deposition. These results demonstrate that the hydrogel-calcium phosphate composite supported the formation of a calcified cartilage-like matrix and is a promising scaffold design for osteochondral interface tissue engineering.

show abstract

Compositional effects on the formation of a calcium phosphate layer and the response of osteoblast-like cells on polymer-bioactive glass composites

Cited by 119 publications

References 43 publications

Composition of chitosan-hydroxyapatite-collagen composite scaffold evaluation after simulated body fluid immersion as reconstruction material

Composition of chitosan-hydroxyapatite-collagen composite scaffold evaluation after simulated body fluid immersion as reconstruction material

In Vitro Study of Dentinal Tubule Occlusion with Sol-gel DP-bioglass for Treatment of Dentin Hypersensitivity

A Hydrogel-Mineral Composite Scaffold for Osteochondral Interface Tissue Engineering

Contact Info

Product

Resources

About