Compositional analysis of the collagenous bone matrix. A study on adult normal and osteopenic bone tissue

Bätge, Boris; Diebold, Joachim; Stein, Heike; Bodó, Michael; Müller, Peter

doi:10.1111/j.1365-2362.1992.tb01450.x

Cited by 53 publications

(27 citation statements)

References 26 publications

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“…To this point, as also suggested by the literature (14)(15)(16)(17), it is worth noting that a changed matrix is certainly relevant to the biological features of bone. Although the main difference between trabecular and cortical bone is the much greater surface area of the former, which explains the differences in bone turnover rates between cortical and trabecular bone, the hypothesis that a modified and possibly defective matrix could somehow be recognized and further removed by osteoclasts cannot be excluded.…”

Section: Discussionmentioning

confidence: 91%

17β-Estradiol and Tamoxifen Prevent the Over-Glycosylation of Rat Trabecular Bone Collagen Induced by Ovariectomy

Moro¹,

Bettica²,

Romanello³

et al. 1997

CCLM

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Summary:We have recently demonstrated that ovariectomy in the rat causes over-glycosylation of collagen which is restricted to trabecular bone. In order to obtain further evidence, we studied whether estrogen or tamoxifen treatment prevented over-glycosylation of trabecular bone collagen. Forty one-hundred-day-old female rats were subjected to ovariectomy (n = 30) or sham-operation (n = 10). Starting the day of the operation, sham-operated rats were treated with vehicle, while ovariectomized rats were divided into three groups and treated with vehicle (n = 10), estrogen (n = 10) or tamoxifen (n = 10). Five rats from each group were sacrificed at 115 and 145 days of age. Femurs and tibiae were separated into cortical and trabecular bone, demineralized, hydrolyzed and analyzed by HPLC for hydroxylysine glycosides and hydroxyproline content. Hydroxylysine glycoside content was expressed as a molar ratio with hydroxyproline. The results can be summarized as follows: 1) cortical bone collagen glycosylation did not vary among the different groups;2) over-glycosylation of trabecular bone collagen observed in the ovariectomized rats was prevented by the administration of either 17ß-estradiol or tamoxifen.These data demonstrated that estrogens affect glycosylation of trabecular bone collagen.

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Section: Discussionmentioning

confidence: 91%

17β-Estradiol and Tamoxifen Prevent the Over-Glycosylation of Rat Trabecular Bone Collagen Induced by Ovariectomy

Moro¹,

Bettica²,

Romanello³

et al. 1997

CCLM

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“…16 In addi tion, the expression of collagen phenotype appeared to be modified since type VI collagen amount tended to decrease. 40 An altered level of lysyl hydroxylation of the alpha 2(1) chain of type I collagen in osteoporotic bone has also been demonstrated by Batge et al 41 Notbohm et al have demonstrated that the thermo stability of type I collagen from osteoporotic patients was reduced and was inversely proportional to their degree of lysyl-overhydroxylation, which could in crease type I collagen susceptibility to proteolytic degradation.42…”

Section: Bone Metabolism After Loss Of Normal Biomechanical Functionmentioning

confidence: 94%

Bone metabolism in spinal cord injured individuals and in others who have prolonged immobilisation. A review

et al. 1995

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Immobilisation or disuse is a condition known to be associated with a decrease in bone mass, osteopenia and in some people leading to osteoporosis with an increased risk of fractures. In this condition, previous histomorphometric and biochemical reports have shown an uncoupling between bone formation and resorption, but the exact sequence of the events resulting in bone loss is still not fully understood. In spinal cord injury for instance, the main finding soon after the onset is decreased osteoblastic activity associated with a dramatic increase in bone degradation. The overall consequence of these metabolic events is the development of a rapid and severe osteoporosis only observed in the paralysed part of the body associated with the loss of biomechanical strength and the biosynthesis of a structurally modified matrix which is unable to sustain normal mechanical stress. This situation dramatically increases the risk of fractures. The same uncoupling phenomenon has been described in healthy individuals who have been submitted to long duration bedrest and also in astronauts during spaceflight; but the timing, intensity and the metabolic subset may be different as these people do recover after cessation of the disuse period, which does not occur in paralysed patients. As new accurate and sensitive non-invasive techniques have become available recently to assess bone and connective tissue metabolism, more information is now available regarding bone loss in paralysed and/or immobilised individuals. These techniques should be definitely helpful in orientating new therapeutic trials with drugs and/or procedures intended to correct the musculoskeletal deleterious effects of disuse. This paper is therefore aimed at a review of bone metabolism in those with a severe spinal cord injury, or with a long duration of bedrest, or with loss of biomechanical function, or with actual or simulated spaceflight, in all instances using non-invasive techniques.

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“…In human, osteoporotic bones, which exhibit decreased quantity and impaired mineralization of bone, have shown lower content of collagen 18,19) and higher extent of Lys hydroxylation [19][20][21][22] be partly damaged compared to those from fresh bone. However, the contents of collagen (average of 125~128 ìg/mg of dried bone)…”

Section: Discussionmentioning

confidence: 99%

Higher Contents of Mineral and Collagen but Lower of Hydroxylysine of Collagen in Mandibular Bone Compared with Those of Humeral and Femoral Bones in Human

Sasaki

Mizutani

Katafuchi

et al. 2010

J. Hard Tissue Biology.

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Minerals and collagen are major components of bone. At present, little is known about the material property of the jaw bone compared to other bones of the body. The purpose of this study was to characterize the material property of mandibular bone expressed by the amount of mineral, collagen, and hydroxylysine (Hyl) in comparison with humeral and femoral bones using human cadavers. Mineral and collagen contents were significantly higher in the mandible than in humerus and femur but Hyl was significantly lower in the mandible. Among the three indices, a weak but significantly positive correlation between collagen and mineral contents was observed. On the contrary, Hyl showed a weak but significantly negative correlation with mineral. The three indices were almost not affected by aging, gender, and dental status which were thought to influence bone quantity. These results suggest that human mandibular bone differs in material property from humeral and femoral bones, and may possess a higher mechanical property explained by the hypothesis that adequate and no excessive extent of lysine hydroxylation of collagen leads to optimal collagen fibrillogenesis and matrix mineralization. The assessment of bone property as shown in this study could disclose unknown characteristics which cannot be expressed by bone quantity.

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Compositional analysis of the collagenous bone matrix. A study on adult normal and osteopenic bone tissue

Cited by 53 publications

References 26 publications

17β-Estradiol and Tamoxifen Prevent the Over-Glycosylation of Rat Trabecular Bone Collagen Induced by Ovariectomy

17β-Estradiol and Tamoxifen Prevent the Over-Glycosylation of Rat Trabecular Bone Collagen Induced by Ovariectomy

Bone metabolism in spinal cord injured individuals and in others who have prolonged immobilisation. A review

Higher Contents of Mineral and Collagen but Lower of Hydroxylysine of Collagen in Mandibular Bone Compared with Those of Humeral and Femoral Bones in Human

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