Composition of black soldier fly prepupae and systematic approaches for extraction and fractionation of proteins, lipids and chitin

Caligiani, Augusta; Leni, Giulia; Baldassarre, Stefania; Maistrello, Lara; Dossena, Arnaldo; Sforza, Stefano

doi:10.1016/j.foodres.2017.12.012

Cited by 238 publications

(211 citation statements)

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“…protein extraction. Grinded insects were defatted with diethyl ether following the method proposed by Caligiani et al 31 using a Soxhlet extractor (SER 148/3 VELP SCIENTIFICA, Usmate Velate, Italy). Defatted flours were subjected to the protein extraction protocol proposed by Abdel Rahman et al 32 with some modifications.…”

Section: Methodsmentioning

confidence: 99%

Shotgun proteomics, in-silico evaluation and immunoblotting assays for allergenicity assessment of lesser mealworm, black soldier fly and their protein hydrolysates

Tedeschi

Faccini

Pratesi

et al. 2020

Sci Rep

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Since 2018, insects have belonged the category of Novel Foods and the presence of allergens represents one of the main hazards connected to their consumption, also due to the potential cross-reactivity with Arthropoda pan-allergens. In the present work, the allergenicity assessment of black soldier fly and lesser mealworm was performed with a shotgun bottom-up proteomic approach combined with in-silico assessment, followed by IgG-and IgE-immunoblotting experiments. The peptides identified, filtered for their abundance and robustness, belonged mainly to muscle proteins, which represented the most abundant protein group. the relevant potential allergens were in-silico identified by sequence similarity to known allergens, and among them tropomyosin resulted the most abundant insect allergen. igG-immunoblotting analysis with anti-tropomyosin i antibodies and ige-immunoblotting assay with serum from patient allergic to crustacean tropomyosin were performed in order to assess the immunoreactivity in both insects. the immunoassays were carried out also on protein hydrolysates extracted by treating insects with protease from Bacillus licheniformis (1%, 60 °C, pH 7.5). While igG-immunoblotting demonstrated the loss of immunoreactivity for both hydrolysates, igeimmunoblotting showed a partial immunoreactivity preservation, also after hydrolysis, in the case of black soldier fly hydrolysate, and a total loss of immunoreactivity for lesser mealworm hydrolysate Novel food protein sources are being studied in order to meet the future requirement for food, in connection to the perspectives of growing population 1 . Insects represent a good source of proteins, not only because of the high protein content in some species, but also for their nutritional quality in terms of essential amino acids profile. Not of a secondary importance, insect breeding, in comparison to common livestock, is characterized by many environmental advantages, such as less land use, feed and water requirement, fewer greenhouse gas emission and high feed conversion ratio 2 .From January 1 st of 2018, insects have been included in the category of novel foods and the European Food Safety Agency (EFSA) opinion is mandatory before their marketing 3 . In 2015, EFSA assessed for the first time the safety related to insect consumption. The potential hazards connected to the use of insects in food or feed were deemed to be related to exogenous and endogenous factors, which could be influenced also by harvesting and processing methods 4 . The presence of allergens represents one of the main endogenous risk related to the consumption of insects. Insect-based food ingredients could cause an allergenic response either due to a primary sensitization, or to a cross-reaction. Primary sensitization is related to the ability of insect proteins to elicit an allergic reaction not related to other food allergies. This risk is widespread mainly in regions where edible insects are commonly used (e.g. China) 5 . A cross reaction, more likely where insects are not commonly consume...

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Section: Methodsmentioning

confidence: 99%

Shotgun proteomics, in-silico evaluation and immunoblotting assays for allergenicity assessment of lesser mealworm, black soldier fly and their protein hydrolysates

Tedeschi

Faccini

Pratesi

et al. 2020

Sci Rep

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“…The BSF prepupae protein fraction were extracted following the procedure reported in a previous study and it is worth noting that the dry matter of BSF prepupae was composed approximately by 32 wt% of protein. [62] The protein fraction was grinded through dry analytical mill (IKA, A10 basic) and sieved in order to ensure the homogeneity of the particle size, below 40 μm, with the aim to equalize the reactive surface available during the polymerization. GL (99%, Sigma Aldrich, St. Louis, Missouri), sodium hydroxide solution (1 M, Sigma Aldrich), sodium hypophosphite (>98%, Sigma Aldrich), and CA (>99.5%, Sigma Aldrich) were employed as additives for protein-based film formulation.…”

Section: Methodsmentioning

confidence: 99%

“…BSF is considered an impressive source of nutrients like proteins, nowadays used as feed for animals, and fats, used in biodiesel production . In addition, BSF is known as a safe waste bio‐converter as it can grow on several types of substrates avoiding any disease transmission risk, increasing its potential relapse in the circular economy perspective . In the present study, proteins derived by BSF prepupae, grown on substrate composed mostly by poultry manure, have been mixed with other components to obtain bioplastics for agricultural purpose.…”

Section: Introductionmentioning

confidence: 99%

“…[57,58] In addition, BSF is known as a safe waste bio-converter as it can grow on several types of substrates avoiding any disease transmission risk, increasing its potential relapse in the circular economy perspective. [59][60][61][62] In the present study, proteins derived by BSF prepupae, grown on substrate composed mostly by poultry manure, have been mixed with other components to obtain bioplastics for agricultural purpose. In this way, insects are used to process a waste and their fractionated prepupae to obtain proteins employed in a biopolymer with potential biodegradability, which can find applications in agricultural field.…”

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Rational design and characterization of bioplastics from Hermetia illucens prepupae proteins

et al. 2018

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In this study proteins extracted from prepupae of Hermetia illucens, also known as black soldier fly, are investigated as promising base for a new type of bioplastics for agricultural purposes. Design of experiments techniques are employed to perform a rational study on the effects of different combination of glycerol as plasticizer, citric acid as cross‐linking agent and distilled water as solvent on the capability of proteins to form a free‐standing film through casting technique, keeping as fixed the quantity of proteins. Glycerol shows interesting properties as plasticizer contributing to the formation of homogenous and free‐standing film. Moreover, mechanical and thermal characterizations are performed to estimate the effect of increasing amounts of proteins on the final properties and thickness of the specimens. Proteins derived from H. illucens can be successfully employed as base for bioplastics to be employed for agricultural purposes.

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“…Мезга перемещается в замкнутом пространстве и с силой проталкивается в более узкое пространство последующего витка. В таких прессах уплотнение материала создается только за счет установки сопротивления в конце прессующего тракта [1].…”

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Analysis of Pressing Equipment for Extraction of Oils and Fats From Agricultural Raw Materials

Maltseva¹,

Serdyuk²

2019

Proceedings of the VII International Scientific and Practical Conference «Innovative Technologies in Science and Education» («I

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Composition of black soldier fly prepupae and systematic approaches for extraction and fractionation of proteins, lipids and chitin

Cited by 238 publications

References 30 publications

Shotgun proteomics, in-silico evaluation and immunoblotting assays for allergenicity assessment of lesser mealworm, black soldier fly and their protein hydrolysates

Shotgun proteomics, in-silico evaluation and immunoblotting assays for allergenicity assessment of lesser mealworm, black soldier fly and their protein hydrolysates

Rational design and characterization of bioplastics from Hermetia illucens prepupae proteins

Analysis of Pressing Equipment for Extraction of Oils and Fats From Agricultural Raw Materials

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