Composition and significance of glycosaminoglycans in the uterus and placenta of mammals

Oliveira, Gleidson Benevides de; Vale, André Menezes do; Santos, Amílton Cesar dos; Moura, Carlos Eduardo Bezerra de; Rocha, Hugo Alexandre Oliveira; Oliveira, Moacir Franco de

doi:10.1590/s1516-8913201500281

Cited by 18 publications

(13 citation statements)

References 65 publications

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“…After the identification of perlecan core protein, we aimed to examine the behavior of this protein in the placenta in normal and pathological conditions throughout gestation since perlecan was previously shown to be involved in cellular functions that are key in placental physiology (Chen et al, 2008; Chang & Vivian Yang, 2013; Oliveira et al, 2015). Interestingly, perlecan was first described in the basement membranes of various normal and tumorous human tissues where it regulated cell attachment and invasion (Hassell et al, 1980; Murdoch et al, 1994), processes also key in placental development.…”

Section: Discussionmentioning

confidence: 99%

Proteomic identification of membrane-associated placental protein 4 (MP4) as perlecan and characterization of its placental expression in normal and pathologic pregnancies

Szenasi

Tóth

Balogh

et al. 2019

PeerJ

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Background More than 50 human placental proteins were isolated and physico-chemically characterized in the 70–80s by Hans Bohn and co-workers. Many of these proteins turned to have important role in placental functions and diagnostic significance in pregnancy complications. Among these proteins was membrane-associated placental protein 4 (MP4), for which identity or function has not been identified yet. Our aim was to analyze the sequence and placental expression of this protein in normal and complicated pregnancies including miscarriage, preeclampsia and HELLP syndrome. Methods Lyophilized MP4 protein and frozen healthy placental tissue were analyzed using HPLC-MS/MS. Placental tissue samples were obtained from women with elective termination of pregnancy (first trimester controls, n = 31), early pregnancy loss (EPL) (n = 13), early preeclampsia without HELLP syndrome (n = 7) and with HELLP syndrome (n = 8), late preeclampsia (n = 8), third trimester early controls (n = 5) and third trimester late controls (n = 9). Tissue microarrays were constructed from paraffin-embedded placentas (n = 81). Slides were immunostained with monoclonal perlecan antibody and evaluated using light microscopy and virtual microscopy. Perlecan was also analyzed for its expression in placentas from normal pregnancies using microarray data. Results Mass spectrometry-based proteomics of MP4 resulted in the identification of basement membrane-specific heparan sulfate proteoglycan core protein also known as perlecan. Immunohistochemistry showed cytoplasmic perlecan localization in syncytiotrophoblast and cytotrophoblasts of the villi. Perlecan immunoscore decreased with gestational age in the placenta. Perlecan immunoscores were higher in EPL compared to controls. Perlecan immunoscores were higher in early preeclampsia without and with HELLP syndrome and lower in late preeclampsia than in respective controls. Among patients with preeclampsia, placental perlecan expression positively correlated with maternal vascular malperfusion and negatively correlated with placental weight. Conclusion Our findings suggest that an increased placental perlecan expression may be associated with hypoxic ischaemic injury of the placenta in miscarriages and in early preeclampsia with or without HELLP syndrome.

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Section: Discussionmentioning

confidence: 99%

Proteomic identification of membrane-associated placental protein 4 (MP4) as perlecan and characterization of its placental expression in normal and pathologic pregnancies

Szenasi

Tóth

Balogh

et al. 2019

PeerJ

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“…They are heteropolysaccharides consisting of repeated units of alternating residues of N-acetylgalactosamine or glycosamine linked to a non-nitrogenated sugar, like uronic acid (D-glycuronic acid or L-iduronic acid) or a galactose, making themselves typical chains are known as mucopolysaccharides, and their association to proteoglycans form the matrix' principal elements playing several physiological and pathological events (Tovar et al, 2005;Volpi, 2011;Oliveira et al, 2015). Chondroitin sulfate (CS), dermatan sulfate (DS), heparan sulfate (HS), keratan sulfate and heparin (HEP) are the most GAGs found, varying not only the hexosamine and non-aminated sugar residue but also the degree and position of sulfated groups, as well as the type of glycoside bond.…”

Section: Introductionmentioning

confidence: 99%

“…Chondroitin sulfate (CS), dermatan sulfate (DS), heparan sulfate (HS), keratan sulfate and heparin (HEP) are the most GAGs found, varying not only the hexosamine and non-aminated sugar residue but also the degree and position of sulfated groups, as well as the type of glycoside bond. Hyaluronic acid does not contain sulfation and may occur freely in tissue (Volpi, 2011;Kang et al, 2015;Oliveira et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Inhibition of thrombin generation by dermatan sulfate isolated from the skin of Oreochromis niloticus

Salles

Rodrigues

Barcellos

et al. 2017

Agraria

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Oreochromis niloticus has skin anticoagulant glycosaminoglycans (GAGs), but their effects on thrombin generation (TG) are unknown. This study partially characterized skin GAGs and analyzed as inhibitors of TG. Papain-extraction yield of 0.1% contained two fractions separated by DEAE-cellulose chromatography, differing on charge density and carboxylated groups by a combination of agarose/polyacrylamide electrophoresis and sequential toluidine blue/stains-all staining, presenting molecular sizes ca. 40 kDa. Depolymerization of the fractions with chondroitin ABC lyase showed dermatan sulfate (DS) as the unique GAG by agarose analysis. Both activated partial thromboplastin time (APTT) and prothrombin time tests only showed anticoagulation by fractions and mammalian DS by APTT (0.61, 0.47 and 1.72 IU, respectively) against heparin (193 IU). Fractions acted concentrationdependent on both intrinsic/extrinsic pathways in TG using 60-fold diluted human plasma, with more than 50% inactivation (41.6 and 83.3 µg), whereas DS and heparin entirely abolished at low amounts. DS from O. niloticus skin blocks in vitro TG in human plasma.Key words: Cichlidae; freshwater fish; sulfated glycans; thrombosis in vitro; waster Inibição da geração de trombina por dermatam sulfato isolado da pele de Oreochromis niloticus RESUMOOreochromis niloticus possui glicosaminoglicanos (GAGs) anticoagulantes de pele, porém desconhecidos são seus efeitos sobre geração de trombina (GT). Caracterizou-se parcialmente GAGs de pele e analisou-se como inibidores de GT. Rendimento de 0,1% da extração com papaína, conteve duas frações separadas por cromatografia de DEAE-celulose, e por combinação de eletroforese de agarose/poliacrilamida e coramento sequencial azul de toluidina/"stains-all", diferiram quanto à densidade da carga e grupos carboxilados, apresentando tamanhos moleculares ca. 40 kDa. Depolimerização das frações com condroitinase ABC mostrou, por análise em agarose, dermatam sulfato (DS) como GAG único. Ambos os testes do tempo de tromboplastina parcial ativada (TTPA) e do tempo de protrombina mostraram anticoagulação, das frações e DS de mamífero, somente pelo TTPA (0,61; 0,47 e 1,72 UI, respectivamente) contra ao da heparina (193 UI). Frações atuaram sobre ambas vias intrínsica/ extrínsica dependente de concentração na GT usando plasma humano diluído 60 vezes, com inativação mais que 50% (41,6 e 83,3 µg), enquanto em quantidades baixas DS e heparina aboliram totalmente. DS da pele de O. niloticus bloqueia GT in vitro no plasma humano. Palavras-chave:Cichlidae; peixe dulcícola; glicanos sulfatados; trombose in vitro; resíduo

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“…Since interfibrillar space and diameter are linked characteristics, they have a joint contribution represented as a single variable. Although GAGs have been found to have no appreciable effect on stiffness, they are known to alter the mechanical structure of the tissue [10,33]. This is probably due to the fact that GAGs affect other components, mainly collagen, so that its action is indirect, and therefore its independent variation in concentration could not quantify a physical interaction with other elements.…”

Section: = ( − )mentioning

confidence: 99%

Histobiomechanical Remodeling of the Cervix during Pregnancy: Proposed Framework

Torres

Faris

Callejas

2019

Mathematical Problems in Engineering

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Pregnancy involves a gradual change in tissue consistency, where, as gestational age increases a drop in stiffness is noticeable. The extracellular matrix (ECM) of the cervix whose behavior is controlled by the collagen structure is reconfigured, due to hormonal and protein related factors that degraded it. There is an increase in cellular and water content. The collagen maintains a stable percentage, although the synthesis-solubility interaction causes a transformation in the network increasing its diameter and waviness, which destabilizes the mechanical integrity. Further understanding of the remodeling of the cervix becomes a key element in the progress against preterm birth. However, since the woman is in a very delicate state, the available information is scattered. The aim is to provide a framework where the variation of the histological properties is linked with the individual biomechanical evolution of the constituents; therefore a multiscale approach to the problem can be made. The results of different authors were reviewed to obtain values of each component depending on the gestational age, and then they were combined with the mechanical development of each variable obtained from the outputs of numerical simulations of another author. An additive function is proposed, in which the mechanical contribution of each constituent is added to a reference value established for a nonpregnant state. Results show how biochemistry models the mechanical behavior of the tissue through the histology and morphology of the ECM.

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Composition and significance of glycosaminoglycans in the uterus and placenta of mammals

Abstract: Glycosaminoglycans (GAGs)

Cited by 18 publications

References 65 publications

Proteomic identification of membrane-associated placental protein 4 (MP4) as perlecan and characterization of its placental expression in normal and pathologic pregnancies

Proteomic identification of membrane-associated placental protein 4 (MP4) as perlecan and characterization of its placental expression in normal and pathologic pregnancies

Inhibition of thrombin generation by dermatan sulfate isolated from the skin of Oreochromis niloticus

Histobiomechanical Remodeling of the Cervix during Pregnancy: Proposed Framework

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