Composite of porous starch-silk fibroin nanofiber-calcium phosphate for bone regeneration

Hadisi, Zhina; Nourmohammadi, Jhamak; Mohammadi, Javad

doi:10.1016/j.ceramint.2015.05.010

Cited by 81 publications

(38 citation statements)

References 37 publications

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“…Additionally, the porosity of the material may play an important role in the water uptake; the substitute is considered a non-porous material, but the ionic exchange with the medium provokes its degradation and the formation of micro-porosities that allow water uptake [6,49]. Furthermore, our water uptake values were lower compared to those obtained in other studies on materials made of fibroin and calcium phosphates, which are usually between 42% and 70% [46,47].…”

Section: Characterization Of Sf/nha Substitutes 1) In Vitro Degradcontrasting

confidence: 54%

“…4c and 4d) were similar among replicates, as in the degradation test, noting the lack of a direct relationship between the concentration of fibroin and the water uptake. Fibroin is a hydrophobic polymer, while HA is hydrophilic [46,47]. Although there is no point of comparison to determine whether these values are favorable or not, a high percentage water uptake is not beneficial in injectable bone substitutes, since it directly influences their degradation by collapsing the material, mainly when it is hydrophilic, as is the case of HA; in this case, the addition of small percentages of fibroin allows a moderate water uptake due to its hydrophobic nature [46,48].…”

Section: Characterization Of Sf/nha Substitutes 1) In Vitro Degradmentioning

confidence: 99%

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Degradation, water uptake, injectability and mechanical strength of injectable bone substitutes composed of silk fibroin and hydroxyapatite nanorods

Buitrago-Vásquez

Orozco

2018

Rev. Fac. Ing.

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Injectable bone substitutes are generally composite materials capable of being extruded through a device; they consist of a solid phase inside a matrix that allows the easy movement of particles. Injectable bone substitutes have the advantage of allowing its application in situ without the need of invasive surgical techniques, guaranteeing a good recovery; for this reason, they are a promising alternative to replace conventional techniques to repair bone defects. Conventional techniques include the use of allografts and autografts, which often cause adverse reactions, and are disadvantageous for both the patient and the doctor. Furthermore, there are no reports regarding bone substitute development in Colombia, creating the necessity to research composite materials that could become injectable bone substitutes. In this study, we manufactured injectable bone substitutes with hydroxyapatite and calcium phosphate, which is most similar to bone tissue, and synthesized them in nanorods with shape and size similar to the natural hydroxyapatite found inside the body. Additionally, we used extracted silk fibroin from silkworm cocoons of Bombyx mori, a natural polymer of protein nature with high mechanical properties and excellent biocompatibility. For the materials manufactured, we evaluated degradation, in a simulated body fluid (SBF) at normal body temperature, water uptake, injectability and mechanical strength. The manufactured bone substitutes showed good degradation and water uptake properties, an approximate 97% injectability, and low mechanical resistance, indicating promising properties to be used as an injectable bone substitute.

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Section: Characterization Of Sf/nha Substitutes 1) In Vitro Degradcontrasting

confidence: 54%

Section: Characterization Of Sf/nha Substitutes 1) In Vitro Degradmentioning

confidence: 99%

Degradation, water uptake, injectability and mechanical strength of injectable bone substitutes composed of silk fibroin and hydroxyapatite nanorods

Buitrago-Vásquez

Orozco

2018

Rev. Fac. Ing.

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“…The uniform biomimetic fiber coatings of CaP can be obtained by mineralization of fibrous scaffold in simulated body fluid (SBF) . Similarly, CaP and CaCO 3 can be precipitated on scaffold by its incubation in salt solutions . In Ref.…”

Section: Introductionmentioning

confidence: 99%

Vaterite coatings on electrospun polymeric fibers for biomedical applications

Savelyeva

Abalymov

Lyubun

et al. 2016

J Biomedical Materials Res

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The process of porous calcium carbonate (CaCO ) covering on electrospun poly(ε-caprolactone) (PCL) fibers is described in this study. Uniform CaCO coatings, composed of vaterite microparticles and its aggregates, were formed on PCL fibers by mineral precipitation from solution under ultrasonic treatment. The porous structure of CaCO in vaterite polymorphic form is useful for loading of various substances (drugs and nanoparticles), and this property makes vaterite an appropriate material for design of drug delivery systems. Such mineralization was implemented to attain therapeutic and/or biological activity of tissue engineering scaffolds based on electrospun PCL, by means of CaCO coatings. Various structures and polymorphs of CaCO coatings were obtained by variation of growth conditions (time of fiber incubation in work solution, ultrasonic treatment of this system). Coating homogeneity, CaCO polymorphic form, morphology, and CaCO mass can be controlled by number of successive stages of fibrous material treatment. Cytotoxicity tests showed that PCL fibers mineralized with CaCO did not release substances toxic for cells. SEM images of PCL/CaCO scaffolds cultured with cells demonstrate that scaffolds supported cell adhesion and spreading. The presented results show the new technique of controlled PCL scaffold mineralization with vaterite, and an opportunity of using PCL/CaCO as scaffolds for tissue engineering. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 94-103, 2017.

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“…The amount of the deposited apatite layer throughout the scaffold was determined by Eq. at different soaking times .

Formed apatite true(normalm normalg true) = W_{1} - (\frac{W_{2}}{1 + S R})

…”

Section: Characterizationmentioning

confidence: 99%

Biomimetic apatite layer formation on a novel citrate starch scaffold suitable for bone tissue engineering applications

et al. 2016

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The formation of biomimetic bone‐like apatite layers throughout the biopolymer‐based hydrogel scaffold is an attractive approach in bone tissue engineering. Here, the starch scaffold was prepared using a combination of particulate leaching and freeze‐drying techniques. The fabricated structures were then modified by citric acid to investigate the formation of bone‐like apatite layer on the porous citrate‐based scaffold after soaking in simulated body fluid (SBF). The Fourier Transform Infrared (FTIR) spectra and X‐ray diffraction (XRD) patterns revealed that the B‐type carbonated apatite has successfully deposited on the scaffold after immersing in SBF for 28 days. Indeed, high chemical affinity of carboxyl group in citrate starch resulted in primary heterogeneous nucleation of apatitic calcium phosphate throughout the starch hydrogel. Moreover, the biological activity of MG63 osteoblast‐like cells cultured on the scaffold was assessed using indirect MTT assay and cell attachment experiment. The results indicated that the cells show significant biocompatibility and cell attachment.

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Composite of porous starch-silk fibroin nanofiber-calcium phosphate for bone regeneration

Cited by 81 publications

References 37 publications

Degradation, water uptake, injectability and mechanical strength of injectable bone substitutes composed of silk fibroin and hydroxyapatite nanorods

Degradation, water uptake, injectability and mechanical strength of injectable bone substitutes composed of silk fibroin and hydroxyapatite nanorods

Vaterite coatings on electrospun polymeric fibers for biomedical applications

Biomimetic apatite layer formation on a novel citrate starch scaffold suitable for bone tissue engineering applications

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