Composite Fiber Spun Mat Synthesis and In Vitro Biocompatibility for Guide Tissue Engineering

Abstract

Composite scaffolds are commonly used strategies and materials employed to achieve similar analogs of bone tissue. This study aims to fabricate 10% wt polylactic acid (PLA) composite fiber scaffolds by the air-jet spinning technique (AJS) doped with 0.5 or 0.1 g of zirconium oxide nanoparticles (ZrO2) for guide bone tissue engineering. ZrO2 nanoparticles were obtained by the hydrothermal method and characterized by X-ray diffraction (XRD) and scanning electron microscopy (SEM). SEM and fourier-transform infrared spectroscopy (FTIR) analyzed the synthesized PLA/ZrO2 fiber scaffolds. The in vitro biocompatibility and bioactivity of the PLA/ZrO2 were studied using human fetal osteoblast cells. Our results showed that the hydrothermal technique allowed ZrO2 nanoparticles to be obtained. SEM analysis showed that PLA/ZrO2 composite has a fiber diameter of 395 nm, and the FITR spectra confirmed that the scaffolds’ chemical characteristics are not affected by the synthesized technique. In vitro studies demonstrated that PLA/ZrO2 scaffolds increased cell adhesion, cellular proliferation, and biomineralization of osteoblasts. In conclusion, the PLA/ZrO2 scaffolds are bioactive, improve osteoblasts behavior, and can be used in tissue bone engineering applications.

β-Tricalcium Phosphate-Modified Aerogel Containing PVA/Chitosan Hybrid Nanospun Scaffolds for Bone Regeneration

“…According to Osorio-Arciniega et al, the critical parameter of the scaffold such as physical, chemical, and surface topography properties can induce cellular recognition signals. Their results showed well attachment of hFOB cells to the polylactic acid/zirconium oxide fiber composite with very spreading, elongated, and lamellae morphology [30]. Our study found that the morphology of the BTCP-AE-FM nanocomposite demonstrated an excellent surface for the attachment of DPSCs as is shown in an SEM image.…”

A review on polylactic acid‐based blends/composites and the role of compatibilizers in biomedical engineering applications

“…It is also noteworthy that the ZrO 2 improve cellular performance and increase hydrophobicity of PLA without affecting their mechanical properties. 432 Arciniega et al 433 used the air-jet spinning technique (AJS) to fabricate PLA/ ZrO 2 fibers of a diameter 395 nm. The surface topography provides a microenvironment that support cell bioactivity and mineral deposits.…”

Polylactide-Based Materials: Synthesis and Biomedical Applications

“…Electrospinning (ES) [ 6 ] and the air-jet spinning technique (AJS) [ 7 ] are the most relevant techniques for the preparation of PLA fibres. Pinese et al [ 6 ] combined the ES process with chemical crosslinking to prepare nanofibers resistant to degradation.…”

“…Most importantly, the obtained materials exhibit acceptable biocompatibility with murine and mouse fibroblasts cells lines that predispose their application in biomedicine. Alvarez-Perez et al [ 7 ] use the AJS technique to develop nanocomposite fibre scaffolds for tissue bone regeneration. The first step towards this aim was the synthesis of ZrO 2 NPs by the hydrothermal method.…”