Complete Genome Sequences of Three
            <i>Mycoplasma anserisalpingitis</i>
            (
            <i>Mycoplasma</i>
            sp. 1220) Strains

Grózner, Dénes; Forró, Barbara; Kovács, Áron Botond; Marton, Szilvia; Bányai, Krisztiàn; Kreizinger, Zsuzsa; Sulyok, Kinga M.; Gyuranecz, Miklós

doi:10.1128/mra.00985-19

Cited by 6 publications

(8 citation statements)

References 11 publications

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“…The MAUVE alignment found more than 86,000 single nucleotide polymorphism (SNPs) and over 9000 of these were only present in MYCAV785, approximately 1% of the whole genome of the strain. Based on the BLAST search done with BRIG and the alignment done by MAUVE, MYCAV785 showed highest similarity (84.67% based on MAUVE) with strain MYCAV93, isolated from the inflammated phallus of a domestic goose in Hungary in 2011 [13] (Fig. 2).…”

Section: Resultsmentioning

confidence: 99%

Isolation of Mycoplasma anserisalpingitidis from swan goose (Anser cygnoides) in China

et al. 2020

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Background: Mycoplasma anserisalpingitidis causes significant economic losses in the domestic goose (Anser anser) industry in Europe. As 95% of the global goose production is in China where the primary species is the swan goose (Anser cygnoides), it is crucial to know whether the agent is present in this region of the world. Results: Purulent cloaca and purulent or necrotic phallus inflammation were observed in affected animals which represented 1-2% of a swan goose breeding flock (75,000 animals) near Guanghzou, China, in September 2019. From twelve sampled animals the cloaca swabs of five birds (three male, two female) were demonstrated to be M. anserisalpingitidis positive by PCR and the agent was successfully isolated from the samples of three female geese. Based on whole genome sequence analysis, the examined isolate showed high genetic similarity (84.67%) with the European isolates. The antibiotic susceptibility profiles of two swan goose isolates, determined by microbroth dilution method against 12 antibiotics and an antibiotic combination were also similar to the European domestic goose ones with tylvalosin and tiamulin being the most effective drugs. Conclusions: To the best of our knowledge this is the first description of M. anserisalpingitidis infection in swan goose, thus the study highlights the importance of mycoplasmosis in the goose industry on a global scale.

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Section: Resultsmentioning

confidence: 99%

Isolation of Mycoplasma anserisalpingitidis from swan goose (Anser cygnoides) in China

et al. 2020

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“…anatis strains. The complete genome sequence of type strain 1220 T was previously published by Gyuranecz’s group [38]. The type strain has a genomic G+C content of 26.6 mol%, and the genome size is 959 110 bp (GenBank accession number CP042295).…”

Section: Full-textmentioning

confidence: 99%

Mycoplasma anserisalpingitidis sp. nov., isolated from European domestic geese (Anser anser domesticus) with reproductive pathology

Volokhov

Grózner

Gyuranecz

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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The GenBank accession numbers for the 16S rRNA gene sequence and the genome of the strain 1220 T are EU597750 and CP042295, respectively, and the GenBank accession numbers for other housekeeping genes used for the genetic characterization of each novel species described in this study are provided in the manuscript. †These authors contributed equally to this work Two supplementary figures, two supplementary tables and one supplementary document are available with the online version of this article.

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“…Next-generation sequencing was performed on NextSeq 500 Illumina equipment (Illumina, Inc., San Diego, CA USA), with NextSeq 500/550 High Output Kit v2.5 reagent kit. Publicly available whole genome sequences of an additional three strains, ATCC BAA-2147, MYCAV93, and MYCAV177 [9] were involved also in the analyses, resulting in 81 strains altogether. The raw sequence reads were first quality checked with the FastQC software version 0.11.8 (downloaded on 2019.02.13) [28], and trimmed with the NxTrimm software version 0.4.3 (downloaded on 2019.02.13) [29] and paired with Geneious Prime version 2019.2.1 (downloaded on 2018.11.12) [30].…”

Section: Strain Preparation and Whole Genome Assemblymentioning

confidence: 99%

“…Symptoms can include phallus and cloaca inflammation, egg infertility, airsacculitis, salpingitis, peritonitis, and embryo lethality [4][5][6]. In recent years the investigation of these bacteria have become a research priority [9]. In 2019 a speciesspecific PCR system was designed [3] that led to improved laboratory diagnosis.…”

Section: Introductionmentioning

confidence: 99%

The core genome multi-locus sequence typing of Mycoplasma anserisalpingitidis

et al. 2020

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Background: Mycoplasma anserisalpingitidis is a waterfowl pathogen that mainly infects geese, can cause significant economic losses and is present worldwide. With the advance of whole genome sequencing technologies, new methods are available for the researchers; one emerging methodology is the core genome Multi-Locus Sequence Typing (cgMLST). The core genome contains a high percentage of the coding DNA sequence (CDS) set of the studied strains. The cgMLST schemas are powerful genotyping tools allowing for the investigation of potential epidemics, and precise and reliable classification of the strains. Although whole genome sequences of M. anserisalpingitidis strains are available, to date, no cgMLST schema has been published for this species. Results: In this study, Illumina short reads of 81 M. anserisalpingitidis strains were used, including samples from Hungary, Poland, Sweden, and China. Draft genomes were assembled with the SPAdes software and analysed with the online available chewBBACA program. User made modifications in the program enabled analysis of mycoplasmas and provided similar results as the conventional SeqSphere+ software. The threshold of the presence of CDS in the strains was set to 93% due to the quality of the draft genomes, resulting in the most accurate and robust schema. Three hundred thirty-one CDSs constituted our cgMLST schema (representing 42,77% of the whole CDS set of M. anserisalpingitidis ATCC BAA-2147), and a Neighbor joining tree was created using the allelic profiles. The correlation was observed between the strains' cgMLST profile and geographical origin; however, strains from the same integration but different locations also showed close relationship. Strains isolated from different tissue samples of the same animal revealed highly similar cgMLST profiles. Conclusions: The Neighbor joining tree from the cgMLST schema closely resembled the real-life spatial and temporal relationships of the strains. The incongruences between background data and the cgMLST profile in the strains from the same integration can be because of the higher probability of contacts between the flocks. This schema can help with the epidemiological investigation and can be used as a basis for further studies.

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Complete Genome Sequences of Three Mycoplasma anserisalpingitis ( Mycoplasma sp. 1220) Strains

Cited by 6 publications

References 11 publications

Isolation of Mycoplasma anserisalpingitidis from swan goose (Anser cygnoides) in China

Isolation of Mycoplasma anserisalpingitidis from swan goose (Anser cygnoides) in China

Mycoplasma anserisalpingitidis sp. nov., isolated from European domestic geese (Anser anser domesticus) with reproductive pathology

The core genome multi-locus sequence typing of Mycoplasma anserisalpingitidis

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