Complete Genome Sequences of Classical Swine Fever Virus Isolates Belonging to a New Subgenotype, 2.1c, from Hunan Province, China

Jiang, Donald Dah-Shyong; Li, Guohua; Gong, Wenjie; Li, Run-Cheng; Hu, Yunfei; Tu, Changchun

doi:10.1128/genomea.00080-12

Cited by 11 publications

(8 citation statements)

References 7 publications

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“…However, Bacillus thuringiensis subsp. kurstaki strain HD73 has only contains one endotoxin gene, the cry1Ac gene, was found to be harbored in pHT73 [ 24 ]. However, B .…”

Section: Discussionmentioning

confidence: 99%

Comparative Analysis of Genomics and Proteomics in Bacillus thuringiensis 4.0718

Rang

Wang

et al. 2015

PLoS ONE

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Bacillus thuringiensis is a widely used biopesticide that produced various insecticidal active substances during its life cycle. Separation and purification of numerous insecticide active substances have been difficult because of the relatively short half-life of such substances. On the other hand, substances can be synthetized at different times during development, so samples at different stages have to be studied, further complicating the analysis. A dual genomic and proteomic approach would enhance our ability to identify such substances, and particularily using mass spectrometry-based proteomic methods. The comparative analysis for genomic and proteomic data have showed that not all of the products deduced from the annotated genome could be identified among the proteomic data. For instance, genome annotation results showed that 39 coding sequences in the whole genome were related to insect pathogenicity, including five cry genes. However, Cry2Ab, Cry1Ia, Cytotoxin K, Bacteriocin, Exoenzyme C3 and Alveolysin could not be detected in the proteomic data obtained. The sporulation-related proteins were also compared analysis, results showed that the great majority sporulation-related proteins can be detected by mass spectrometry. This analysis revealed Spo0A~P, SigF, SigE(+), SigK(+) and SigG(+), all known to play an important role in the process of spore formation regulatory network, also were displayed in the proteomic data. Through the comparison of the two data sets, it was possible to infer that some genes were silenced or were expressed at very low levels. For instance, found that cry2Ab seems to lack a functional promoter while cry1Ia may not be expressed due to the presence of transposons. With this comparative study a relatively complete database can be constructed and used to transform hereditary material, thereby prompting the high expression of toxic proteins. A theoretical basis is provided for constructing highly virulent engineered bacteria and for promoting the application of proteogenomics in the life sciences.

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“…However, Bacillus thuringiensis subsp. kurstaki strain HD73 has only contains one endotoxin gene, the cry1Ac gene, was found to be harbored in pHT73 [ 24 ]. However, B .…”

Section: Discussionmentioning

confidence: 99%

Comparative Analysis of Genomics and Proteomics in Bacillus thuringiensis 4.0718

Rang

Wang

et al. 2015

PLoS ONE

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“…These strains had so far been placed into sub-genotype 1.2 but formed a distinct cluster when compared based on longer genome fragments, e.g., full-length E2, N pro , C, E1, and E rns [ 43 ]. Further divisions that have been proposed concern sub-genotypes 2.1 and 2.3 [ 44 , 45 , 46 , 47 ]. However, these systems of clusters or clades were not further harmonized and did not enter routine use.…”

Section: Virus Propertiesmentioning

confidence: 99%

Classical Swine Fever—An Updated Review

Blome

Staubach

Henke

et al. 2017

Viruses

192

217

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Classical swine fever (CSF) remains one of the most important transboundary viral diseases of swine worldwide. The causative agent is CSF virus, a small, enveloped RNA virus of the genus Pestivirus. Based on partial sequences, three genotypes can be distinguished that do not, however, directly correlate with virulence. Depending on both virus and host factors, a wide range of clinical syndromes can be observed and thus, laboratory confirmation is mandatory. To this means, both direct and indirect methods are utilized with an increasing degree of commercialization. Both infections in domestic pigs and wild boar are of great relevance; and wild boars are a reservoir host transmitting the virus sporadically also to pig farms. Control strategies for epidemic outbreaks in free countries are mainly based on classical intervention measures; i.e., quarantine and strict culling of affected herds. In these countries, vaccination is only an emergency option. However, live vaccines are used for controlling the disease in endemically infected regions in Asia, Eastern Europe, the Americas, and some African countries. Here, we will provide a concise, updated review on virus properties, clinical signs and pathology, epidemiology, pathogenesis and immune responses, diagnosis and vaccination possibilities.

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“…Bt strain HD73 (accession no. CP004069 ) was used in this study (Liu et al, 2013 ). The Bt strains were transformed by electroporation, as previously described (Lereclus et al, 1989 ).…”

Section: Methodsmentioning

confidence: 99%

Disruption of Two-component System LytSR Affects Forespore Engulfment in Bacillus thuringiensis

Peng

Chen

et al. 2017

Front. Cell. Infect. Microbiol.

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Two-component regulatory systems (TCSs) play pivotal roles in bacteria sensing many different stimuli from environment. Here, we investigated the role of the LytSR TCS in spore formation in Bacillus thuringiensis (Bt) subsp. kurstaki HD73. lacZ gene fusions revealed that the transcription of the downstream genes, lrgAB, encoding two putative membrane-associated proteins, is regulated by LytSR. The sporulation efficiency of a lytSR mutant was significantly lower than that of wild-type HD73. A confocal microscopic analysis demonstrated that LytSR modulates the process of forespore engulfment. Moreover, the transcription of the lytSR operon is regulated by the mother-cell transcription factor SigE, whereas the transcription of the sporulation gene spoIIP was reduced in the lytSR mutant, as demonstrated with a β-galactosidase activity assay. These results suggest that LytSR modulates forespore engulfment by affecting the transcription of the spoIIP gene in Bt.

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Complete Genome Sequences of Classical Swine Fever Virus Isolates Belonging to a New Subgenotype, 2.1c, from Hunan Province, China

Cited by 11 publications

References 7 publications

Comparative Analysis of Genomics and Proteomics in Bacillus thuringiensis 4.0718

Comparative Analysis of Genomics and Proteomics in Bacillus thuringiensis 4.0718

Classical Swine Fever—An Updated Review

Disruption of Two-component System LytSR Affects Forespore Engulfment in Bacillus thuringiensis

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