Disruption of Two-component System LytSR Affects Forespore Engulfment in Bacillus thuringiensis

Peng, Qi; Wu, Jianbo; Chen, Xiaomin; Qiu, Lili; Zhang, Jie; Tian, Hongtao; Song, Fuping

doi:10.3389/fcimb.2017.00468

Cited by 7 publications

(2 citation statements)

References 41 publications

(53 reference statements)

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“…Confocal laser-scanning microscopy was performed as previously described [23]. Briefly, cells were stained with the membrane dye FM4-64 (final concentration 100 µM; Molecular Probes, Inc., Eugene, OR, USA).…”

Section: Confocal Laser-scanning Microscopymentioning

confidence: 99%

SpoVG Is Necessary for Sporulation in Bacillus anthracis

Chen¹,

Lyu²,

Feng³

et al. 2020

Microorganisms

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The Bacillus anthracis spore constitutes the infectious form of the bacterium, and sporulation is an important process in the organism’s life cycle. Herein, we show that disruption of SpoVG resulted in defective B. anthracis sporulation. Confocal microscopy demonstrated that a ΔspoVG mutant could not form an asymmetric septum, the first morphological change observed during sporulation. Moreover, levels of spoIIE mRNA were reduced in the spoVG mutant, as demonstrated using β-galactosidase activity assays. The effects on sporulation of the ΔspoVG mutation differed in B. anthracis from those in B. subtilis because of the redundant functions of SpoVG and SpoIIB in B. subtilis. SpoVG is highly conserved between B. anthracis and B. subtilis. Conversely, BA4688 (the protein tentatively assigned as SpoIIB in B. anthracis) and B. subtilis SpoIIB (SpoIIBBs) share only 27.9% sequence identity. On complementation of the B. anthracis ΔspoVG strain with spoIIBBs, the resulting strain pBspoIIBBs/ΔspoVG could not form resistant spores, but partially completed the prespore engulfment stage. In agreement with this finding, mRNA levels of the prespore engulfment gene spoIIM were significantly increased in strain pBspoIIBBs/ΔspoVG compared with the ΔspoVG strain. Transcription of the coat development gene cotE was similar in the pBspoIIBBs/ΔspoVG and ΔspoVG strains. Thus, unlike in B. subtilis, SpoVG appears to be required for sporulation in B. anthracis, which provides further insight into the sporulation mechanisms of this pathogen.

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Section: Confocal Laser-scanning Microscopymentioning

confidence: 99%

SpoVG Is Necessary for Sporulation in Bacillus anthracis

Chen¹,

Lyu²,

Feng³

et al. 2020

Microorganisms

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“…The vital membrane dye FM 4-64 (Molecular Probes, Inc., Eugene, OR, USA) or MitoTracker green FM (ThermoFisher, USA) was dissolved in dimethyl sulfoxide ( 35 ). To assess polar septa and engulfment, 1-ml amounts of cells cultured to a designated time point in SSM medium were pelleted and resuspended in 0.02 to 0.05 ml H 2 O. Aliquots (2 μl) of the cell suspensions were placed on slides, stained with FM 4-64 (100 μM) and MitoTracker green FM (MTG; 100 nM) for 1 min ( 47 ), and then scanned with a confocal laser scanning microscope (Leica TCS SL; Leica Microsystems, Wetzlar, Germany) ( 24 ). Each strain was scanned independently at least three times, and each scan was then viewed in at least five fields.…”

Section: Methodsmentioning

confidence: 99%

Identification and Functional Characterization of Two Homologous SpoVS Proteins Involved in Sporulation of Bacillus thuringiensis

et al. 2021

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Cell death dependent on holins LrgAB repressed by a novel ArsR family regulator CdsR

Zhang,

Chen,

Yan

et al. 2024

Cell Death Discov.

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The cell death and survival paradox in various biological processes requires clarification. While spore development causes maternal cell death in Bacillus species, the involvement of other cell death pathways in sporulation remains unknown. Here, we identified a novel ArsR family transcriptional regulator, CdsR, and found that the deletion of its encoding gene cdsR causes cell lysis and inhibits sporulation. To our knowledge, this is the first report of an ArsR family transcriptional regulator governing cell death. We found that CdsR directly repressed lrgAB expression. Furthermore, lrgAB overexpression resulted in cell lysis without sporulation, akin to the cdsR mutant, suggesting that LrgAB, a holin-like protein, induces cell death in Bacillus spp. The lrgAB mutation increases abnormal cell numbers during spore development. In conclusion, we propose that a novel repressor is vital for inhibiting LrgAB-dependent cell lysis.

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Disruption of Two-component System LytSR Affects Forespore Engulfment in Bacillus thuringiensis

Cited by 7 publications

References 41 publications

SpoVG Is Necessary for Sporulation in Bacillus anthracis

SpoVG Is Necessary for Sporulation in Bacillus anthracis

Identification and Functional Characterization of Two Homologous SpoVS Proteins Involved in Sporulation of Bacillus thuringiensis

Cell death dependent on holins LrgAB repressed by a novel ArsR family regulator CdsR

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