Complete Genome Sequence of Finegoldia magna, an Anaerobic Opportunistic Pathogen

Abstract

Finegoldia magna (formerly Peptostreptococcus magnus), a member of the Gram-positive anaerobic cocci (GPAC), is a commensal bacterium colonizing human skin and mucous membranes. Moreover, it is also recognized as an opportunistic pathogen responsible for various infectious diseases. Here, we report the complete genome sequence of F. magna ATCC 29328. The genome consists of a 1 797 577 bp circular chromosome and an 189 163 bp plasmid (pPEP1). The metabolic maps constructed based on the genome information confirmed that most F. magna strains cannot ferment most sugars, except fructose, and have various aminopeptidase activities. Three homologs of albumin-binding protein, a known virulence factor useful for antiphagocytosis, are encoded on the chromosome, and one albumin-binding protein homolog is encoded on the plasmid. A unique feature of the genome is that F. magna encodes many sortase genes, of which substrates may be involved in bacterial pathogenesis, such as antiphagocytosis and adherence to the host cell. The plasmid pPEP1 encodes seven sortase and seven substrate genes, whereas the chromosome encodes four sortase and 19 substrate genes. These plasmid-encoded sortases may play important roles in the pathogenesis of F. magna by enriching the variety of cell wall anchored surface proteins.

Antimicrobial Susceptibility of Clinically Relevant Gram-Positive Anaerobic Cocci Collected over a Three-Year Period in the Netherlands

“…asaccharolyticus (10,21). The combination of diminished antimicrobial susceptibility, its prevalence, and the described virulence factors (8) gives F. magna a special position among the GPAC. …”

Gram-positive anaerobic cocci – commensals and opportunistic pathogens

“…The GA module is found in the Peptostreptococcal Albumin Binding (PAB) protein, originally isolated from F. magna in 1994 (de Château & Björck, 1994). The presence of seven sortase genes on the plasmid seems to be a unique feature of F. magna, as searching of sortases in 29 plasmids of 14 Gram-positive bacterial species revealed only one sortase gene present on a Clostridium perfringens plasmid (Goto et al, 2008). Also, collagen adhesion homologues, amidase homologues, a serine proteinase precursor and a putative biofilm-associated surface protein were identified.…”

Genome Sequence of Peptoniphilus rhinitidis 1-13 T , an Anaerobic Coccus Strain Isolated from Clinical Specimens

“…he Gram-positive anaerobic cocci (GPAC) are a major part of the normal flora and are isolated predominantly from the skin, mouths, and gastrointestinal, urinary, and vaginal tracts of humans (7). GPAC are associated with a variety infections as opportunists, constituting about 20 to 40% of the anaerobes isolated from clinical specimens (3,10).…”

“…The draft genome sequence of P. rhinitidis [1][2][3][4][5][6][7][8][9][10][11][12][13] T is available in GenBank under accession numbers BAEW01000001 to BAEW01000056.…”