Complement component C3 transcription in Atlantic halibut (Hippoglossus hippoglossus L.) larvae

Lange, Sigrun; Bambir, Slavko H.; Dodds, Alister W.; Bowden, Tim; Bricknell, Ian; Espelid, Sigrun; Magnadóttir, Bergljót

doi:10.1016/j.fsi.2005.05.004

Cited by 37 publications

(24 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly, an early appearance of macrophages has been described in three marine species, yellowtail, sea bream and Japanese flounder [176]. The ontogeny of the complement component C3 of cod and halibut has recently been described using immunohistology [181,182]. In both instances C3 was present in several organs at hatching.…”

Section: The Ontogeny Of the Innate Immune Systemmentioning

confidence: 83%

Innate immunity of fish (overview)

Magnadóttir

2006

Fish & Shellfish Immunology

Self Cite

1,741

809

View full text Add to dashboard Cite

Section: The Ontogeny Of the Innate Immune Systemmentioning

confidence: 83%

Innate immunity of fish (overview)

Magnadóttir

2006

Fish & Shellfish Immunology

Self Cite

1,741

809

View full text Add to dashboard Cite

“…These genes included three complement components (i.e., C3-1, C3-2 and Bf/C2), two cytokines (i.e., IL-21 and type I IFN [IFN]), lysozyme (LZM), novel immune-type receptor (NITR-18), Ikaros (IK) and ceruloplasmin (CP). It is well known that the complement system is one of the first lines of immune defense as well as a modifier of acquired immunity [18,29]. The complement system is activated though three different but partially overlapping routes: the classical pathway, the alternative pathway and the lectin pathway [19,29].…”

Section: Introductionmentioning

confidence: 99%

“…It is well known that the complement system is one of the first lines of immune defense as well as a modifier of acquired immunity [18,29]. The complement system is activated though three different but partially overlapping routes: the classical pathway, the alternative pathway and the lectin pathway [19,29]. Complement C3 is the central protein of all three activation pathways and the major opsonin of the complement system, which is also essential for the generation of the membrane attack complex [29].…”

Section: Introductionmentioning

confidence: 99%

“…The complement system is activated though three different but partially overlapping routes: the classical pathway, the alternative pathway and the lectin pathway [19,29]. Complement C3 is the central protein of all three activation pathways and the major opsonin of the complement system, which is also essential for the generation of the membrane attack complex [29]. Besides complement C3, other components, such as complement C2 and factor B, also play important roles in the innate immune response.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Ontogenetic expression and 17β-estradiol regulation of immune-related genes in early life stages of Japanese medaka (Oryzias latipes)

Sun

Shao

et al. 2011

Fish & Shellfish Immunology

View full text Add to dashboard Cite

“…However, the adaptative immune response of halibut is not mature at the first larval stage of feeding (Øvergård et al, 2010). Therefore, the innate (nonspecific) response of the immune system of halibut has to be used for protection against pathogens at this stage (Lange et al, 2006;Magnadottir, 2006). For this reason, we have used the innate immune response associated with the expression of certain effectors obtained after genomic approaches.…”

Section: Introductionmentioning

confidence: 99%

Characterization of culturable bacterial flora in yolk sac larvae of Atlantic halibut (Hippoglossus hippoglossus L.) with

Urtubia¹,

Gallardo²,

Lavín³

et al. 2014

lajar

View full text Add to dashboard Cite

ABSTRACT.One of the main problems facing Atlantic halibut hatcheries is the high mortality in the early stages of larval development. Several factors could be involved, for example: water quality, diseases or abnormalities, such as deformities occurring in the yolk sac larvae prior to exogenous feeding. The aim of this study was to identify differences in bacterial flora associated with yolk sac larvae with oral deformity. We also aimed to establish whether there is any relationship between bacterial strains and the "gaping jaws" syndrome. During our study, 74 bacterial isolates were obtained using three different nutrient media: Marine Agar, R2A and TCBS. Some of these bacteria were characterized using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) and 16S rRNA sequencing. The immune response in larvae exhibiting the "gaping jaws" condition was measured by real time PCR. Our results showed significant differences in bacterial flora between normal and gaping larvae. The gaping yolk sac larvae were predominantly colonized by members of the families Vibrionaceae and Flavobacteriaceae. Bacteria belonging to the Bacillus and Pseudoalteromonas genera were also present but less frequent. It was not possible to associate a type or group of bacteria directly related to "gaping". Strikingly, larvae with gaping jaws had an increase in the expression of two immune related genes, like hepcidin and chemokine (MIP-1ß). These results indicate activation of the immune response in larvae with "gaping jaws" syndrome and this response could be related to bacteria isolated from gaping condition. Keywords: Atlantic halibut, Hippoglossus hippoglossus, bacterial flora, "gaping jaws", immune genes.Caracterización de la flora cultivable y la respuesta inmune en larvas con saco vitelino del halibut del Atlántico (Hippoglossus hippoglossus L.) con el síndrome de "gaping jaws" RESUMEN. Uno de los mayores problemas que enfrentan los criaderos de halibut del Atlántico es la alta mortalidad en estados tempranos del desarrollo larval. Distintos factores pueden estar involucrados, e.g., calidad del agua, enfermedades o anormalidades tales como deformidades que ocurren en las larvas en estado de saco vitelino antes de la alimentación exógena. El objetivo de este estudio fue identificar diferencias en la flora bacteriana asociada a larvas con saco vitelino con deformidad oral. Además se buscó establecer si existió relación entre alguna cepa bacteriana y el síndrome de "gaping jaws". Durante el estudio, 74 cepas bacterianas fueron aisladas usando diferentes medios de cultivo: agar marino, R2A y agar TCBS. Algunas de las bacterias fueron caracterizadas usando reacción en cadena de la polimerasa con análisis del polimorfismo de los fragmentos de restricción (PCR-RFLP) y secuenciación del gen ribosomal 16S. La respuesta inmune de larvas con el síndrome de "gaping jaws" fue medida por PCR en tiempo real. Los resultados evidenciaron diferencias significativas en la flora entre larvas normales y "gaping jaws". Las larvas c...

show abstract

Complement component C3 transcription in Atlantic halibut (Hippoglossus hippoglossus L.) larvae

Cited by 37 publications

References 41 publications

Innate immunity of fish (overview)

Innate immunity of fish (overview)

Ontogenetic expression and 17β-estradiol regulation of immune-related genes in early life stages of Japanese medaka (Oryzias latipes)

Characterization of culturable bacterial flora in yolk sac larvae of Atlantic halibut (Hippoglossus hippoglossus L.) with

Contact Info

Product

Resources

About