Ontogenetic expression and 17β-estradiol regulation of immune-related genes in early life stages of Japanese medaka (Oryzias latipes)

Sun, Liwei; Shao, Xiaolu; Wu, Yuangang; Li, Jingming; Zhou, Qinfang; Lin, Bo; Bao, Shenyuan; Fu, Zhengwei

doi:10.1016/j.fsi.2011.02.020

Cited by 26 publications

(11 citation statements)

References 43 publications

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“…These results explained the important leucocyte mortality enhancement due to necrosis, without specific impact on one leucocyte population. This hypothesis was supported by other authors who showed sexual hormones induced an increase of different macrophage cytokines, which initiate or regulate inflammatory process (Jin et al, 2010;Sun et al, 2011). In another way, since an increase of ROS production could on a long term result in apoptotic cell death (Risso-de Faverney et al, 2001;Krumschnabel et al, 2005), the absence of apoptosis here seems to be due to stable ROS basal levels, which insinuates an absence of oxidative stress.…”

Section: Discussionsupporting

confidence: 74%

Detection of immunotoxic effects of estrogenic and androgenic endocrine disrupting compounds using splenic immune cells of the female three-spined stickleback, Gasterosteus aculeatus (L.)

Bado‐Nilles¹,

Técher²,

Porcher³

et al. 2014

Environmental Toxicology and Pharmacology

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Today, the list of endocrine disrupting compounds (EDCs) in freshwater and marine environments that mimic or block endogenous hormones is expanding at an alarming rate. As immune and reproductive systems may interact in a bidirectional way, some authors proposed the immune capacities as attractive markers to evaluate the hormonal potential of environmental samples. Thus, the present work proposed to gain more knowledge on direct biological effects of natural and EDCs on female fish splenic leucocyte non-specific immune activities by using ex vivo assays. After determining the optimal required conditions to analyze splenic immune responses, seven different EDCs were tested ex vivo at 0.01, 1 and 100nM over 12h on the leucocyte functions of female three-spined stickleback, Gasterosteus aculeatus. In summary, we found that natural hormones acted as immunostimulants, whilst EDCs were immunosuppressive.

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Section: Discussionsupporting

confidence: 74%

Detection of immunotoxic effects of estrogenic and androgenic endocrine disrupting compounds using splenic immune cells of the female three-spined stickleback, Gasterosteus aculeatus (L.)

Bado‐Nilles¹,

Técher²,

Porcher³

et al. 2014

Environmental Toxicology and Pharmacology

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“…Inhibition of E2 sulfonation will sustain the level of bioactive estradiol and lead to a prolonged estrogenic effect (Fisher 2004). A number of recent studies have shown that an estrogen-active compound, e.g., E2, is able to downregulate complement system and C3 transcription in juvenile/male fish (Williams et al 2007; Sun et al 2011; Wenger et al 2011). The suppression effect of BDE-47 on C3 and complement gene transcription in BDE-47 exposed male O. melastigma could be attributed to an increase of estrogenic effect induced by BDE-47.…”

Section: Discussionmentioning

confidence: 99%

“…Complement component mRNA expression, for instance, C1q, C3, and C7 of juvenile/male fish, is highly responsive to estrogen-active chemicals (see review of Casanova-Nakayama et al 2011). Furthermore, larvae of Japanese medaka ( O. latipes ) exposed to 17β-estradiol (0.1–10 μg/L for 30 days) showed a concentration- and time-dependent suppression in mRNA expression of three complement components (C3-1, C3-2, and Bf/C2) (Sun et al 2011). Similar analyses were not conducted for mature female fish with a high endogenous level of estrogen.…”

Section: Introductionmentioning

confidence: 99%

Gender-specific modulation of immune system complement gene expression in marine medaka Oryzias melastigma following dietary exposure of BDE-47

Lei

Lam

et al. 2012

Environ Sci Pollut Res

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BDE-47 is one of the most widely found congeners of PBDEs in marine environments. The potential immunomodulatory effects of BDE-47 on fish complement system were studied using the marine medaka Oryzias melastigma as a model fish. Three-month-old O. melastigma were subjected to short-term (5 days) and long-term (21 days) exposure to two concentrations of BDE-47 (low dose at 290 ± 172 ng/day; high dose at 580 ± 344 ng/day) via dietary uptake of BDE-47 encapsulated in Artemia nauplii. Body burdens of BDE-47 and other metabolic products were analyzed in the exposed and control fish. Only a small amount of debrominated product, BDE-28, was detected, while other metabolic products were all under detection limit. Transcriptional expression of six major complement system genes involved in complement activation: C1r/s (classical pathway), MBL-2 (lectin pathway), CFP (alternative pathway), F2 (coagulation pathway), C3 (the central component of complement system), and C9 (cell lysis) were quantified in the liver of marine medaka. Endogenous expression of all six complement system genes was found to be higher in males than in females (p < 0.05). Upon dietary exposure of marine medaka to BDE-47, expression of all six complement genes were downregulated in males at day 5 (or longer), whereas in females, MBl-2, CFP, and F2 mRNAs expression were upregulated, but C3 and C9 remained stable with exposure time and dose. A significant negative relationship was found between BDE-47 body burden and mRNA expression of C1r/s, CFP, and C3 in male fish (r = −0.8576 to −0.9447). The above findings on changes in complement gene expression patterns indicate the complement system may be compromised in male O. melastigma upon dietary exposure to BDE-47. Distinct gender difference in expression of six major complement system genes was evident in marine medaka under resting condition and dietary BDE-47 challenge. The immunomodulatory effects of BDE-47 on transcriptional expression of these complement components in marine medaka were likely induced by the parent compound instead of biotransformed products. Our results clearly demonstrate that future direction for fish immunotoxicology and risk assessment of immunosuppressive chemicals must include parallel evaluation for both genders.Electronic supplementary materialThe online version of this article (doi:10.1007/s11356-012-0887-z) contains supplementary material, which is available to authorized users.

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“…Fish transcriptomes have been analyzed during sexual differentiation [ 20 – 25 ] and after E 2 exposure [ 18 , 26 – 28 ]. Tissue- and gender-specific responses, [ 29 , 30 ] as well as biogeographical differences [ 31 ], have been shown. However, it is still not clear whether the exposure to exogenous steroids elicits changes similar to those occurring during natural physiological processes [ 32 – 34 ].…”

Section: Introductionmentioning

confidence: 99%

Estrogen exposure overrides the masculinizing effect of elevated temperature by a downregulation of the key genes implicated in sexual differentiation in a fish with mixed genetic and environmental sex determination

Díaz

Piferrer

2017

BMC Genomics

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BackgroundUnderstanding the consequences of thermal and chemical variations in aquatic habitats is of importance in a scenario of global change. In ecology, the sex ratio is a major population demographic parameter. So far, research that measured environmental perturbations on fish sex ratios has usually involved a few model species with a strong genetic basis of sex determination, and focused on the study of juvenile or adult gonads. However, the underlying mechanisms at the time of gender commitment are poorly understood. In an effort to elucidate the mechanisms driving sex differentiation, here we used the European sea bass, a fish species where genetics and environment (temperature) contribute equally to sex determination.ResultsHere, we analyzed the transcriptome of developing gonads experiencing either testis or ovarian differentiation as a result of thermal and/or exogenous estrogen influences. These external insults elicited different responses. Thus, while elevated temperature masculinized genetic females, estrogen exposure was able to override thermal effects and resulted in an all-female population. A total of 383 genes were differentially expressed, with an overall downregulation in the expression of genes involved in both in testicular and ovarian differentiation when fish were exposed to Estradiol-17ß through a shutdown of the first steps of steroidogenesis. However, once the female phenotype was imposed, gonads could continue their normal development, even taking into account that some of the resulting females were fish that otherwise would have developed as males.ConclusionsThe data on the underlying mechanisms operating at the molecular level presented here contribute to a better understanding of the sex ratio response of fish species subjected to a combination of two of the most common environmental perturbations and can have implications in future conservational policies.

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Ontogenetic expression and 17β-estradiol regulation of immune-related genes in early life stages of Japanese medaka (Oryzias latipes)

Cited by 26 publications

References 43 publications

Detection of immunotoxic effects of estrogenic and androgenic endocrine disrupting compounds using splenic immune cells of the female three-spined stickleback, Gasterosteus aculeatus (L.)

Detection of immunotoxic effects of estrogenic and androgenic endocrine disrupting compounds using splenic immune cells of the female three-spined stickleback, Gasterosteus aculeatus (L.)

Gender-specific modulation of immune system complement gene expression in marine medaka Oryzias melastigma following dietary exposure of BDE-47

Estrogen exposure overrides the masculinizing effect of elevated temperature by a downregulation of the key genes implicated in sexual differentiation in a fish with mixed genetic and environmental sex determination

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