Competing endogenous RNA network analysis of CD274, IL‑10 and FOXP3 co‑expression in laryngeal squamous cell carcinoma

Abstract

Laryngeal squamous cell carcinoma (LSCC) is one of the most common types of head and neck malignant tumor; however, there is a lack of effective molecular targets for therapy. The present study detected the expression of three immunity‑associated molecules [forkhead box (FOX)3, interleukin (IL)‑10 and cluster of differentiation (CD)274] in 133 LSCC samples using immunohistochemistry (IHC); subsequently, the association between their expression and the clinical characteristics of LSCC were analyzed. Spearman's rank correlation method, Kaplan‑Meier and Cox regression model were used to analyze the correlations of the three proteins and their clinical significance. StarBase and miRTarBase databases were used to establish the competitive endogenous (ce)RNA network of the three molecules. IHC demonstrated that the positive expression rates of FOXP3, IL‑10 and CD274 were 68.4, 73.7 and 58.6% in 133 LSCC samples, respectively. In addition, it was identified that the expression of the three proteins was closely correlated with the clinical characteristics of LSCC, including lymph node metastasis and prognosis (P<0.05). There was also a significant association of co‑expression between any two proteins (P<0.001). Furthermore, the expression levels of FOXP3, IL‑10 and CD274 were negatively associated with the survival rate of patients with LSCC (P<0.05). The results of a Cox regression model suggested that the three proteins were prognostic risk factors for LSCC (P<0.05). The ceRNA network revealed that 10 microRNAs (miRs; including miR‑16‑5p and miR‑214‑3p), 123 long non‑coding RNAs (including X‑inactive specific transcript, H19 and metastasis associated lung adenocarcinoma transcript 1) and 408 circular RNAs (including ATP‑binding cassette subfamily C member 1 hsa_circ_001569 and ISY1 splicing factor homolog hsa_circ_001859) may regulate the expression of FOXP3, IL‑10 and CD274. The data generated from the present study may increase the understanding of the immune escape mechanisms of LSCC and may be beneficial for the development of a specific immunotherapy.

A prognostic long non-coding RNA-associated competing endogenous RNA network in head and neck squamous cell carcinoma

“…Previous publications have focused in part on ceRNAs in laryngeal squamous cell carcinoma (LSCC) (Kong et al, 2019;Liu & Ye, 2019;Gao et al, 2019;Zhao et al, 2019;Sun et al, 2018;Feng et al, 2016), tongue squamous cell carcinoma (TSCC) (Sturgis & Cinciripini, 2007), and nasopharyngeal carcinoma (NPC) (Fullerton et al, 2020;Agrawal et al, 2011). These studies have identified differentially expressed RNAs by lncRNA and mRNA integrated microarrays of cancer tissue or by immunohistochemistry (Gao et al, 2019;Zhao et al, 2019;Sun et al, 2018;Feng et al, 2016). The different key RNAs screened may be attributed to the patients included from different databases and the different tumor types.…”

Differential expression of lncRNA/miRNA/mRNA and their related functional networks during the osteogenic/odontogenic differentiation of dental pulp stem cells

“…Through MREs, lncRNAs, mRNAs, and miRNAs form ceRNA cross-talk systems (J. Sun et al, 2018). The physiological consequences of ceRNAs in systemic lupus erythematosus, cancer, and other diseases have been reported, thus representing novel treatment strategies (L. J.…”

Retracted: Long Noncoding RNA SOX2OT Accelerates the Carcinogenesis of Wilms' Tumor Through ceRNA Through miR-363/FOXP4 Axis

“…Forkhead-box (FOX) gene family consists of > 43 members (Yamazaki et al, 2018;Sun et al, 2018b). The transcription factors of FOX family also epigenetically regulate the protein gene expression in cells, thereby modulating the cellular progression (Yin et al, 2017;Hu et al, 2018).…”