Differential expression of lncRNA/miRNA/mRNA and their related functional networks during the osteogenic/odontogenic differentiation of dental pulp stem cells

Liu, Zhongjun; Xu, Shuaimei; Dao, Junfeng; Zhang, Gan; Zeng, Xiongqun

doi:10.1002/jcp.29223

Cited by 47 publications

(45 citation statements)

References 41 publications

(40 reference statements)

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“…In addition, miR-138, miR-31, miR-142, miR-148, and miR-637 could inhibit the expression of OSX (Zhang et al, 2012;Liu et al, 2020). Chen et al (2016) found that serum miR-30b-5p level was significantly down-regulated in postmenopausal women, and the results were consistent in the corresponding rat model.…”

Section: Other Mirnasmentioning

confidence: 61%

“…Promote osteoblastic differentiation (Hassan et al, 2012) miR-143, miR-31 OSX Inhibit osteoblastic differentiation (Zhang et al, 2012;Li E. et al, 2014;Liu et al, 2020) miR-101, miR-132 PI3K/AKT/mTOR pathway Promote osteoblastic differentiation (Qi and Zhang, 2014) miR-17 TCF/Wnt pathway Inhibit osteoblastic differentiation (Liu et al, 2013) miR-216 PI3K/AKT pathway Promote osteoblastic differentiation (Xiao et al, 2016) miR-194 STAT1 Promote osteoblastic differentiation (Li et al, 2015) miR-96 EGFR signaling Promote osteoblastic differentiation (Yang et al, 2014) miR-23 MARK pathway Inhibit osteoblastic differentiation (Jiang et al, 2020) miR-375 RUNX2 Inhibit osteoblastic differentiation (Du et al, 2015) miR-153 BMPRII Inhibit osteoblastic differentiation (Cao et al, 2015) miR-124 DLX Inhibit osteoblastic differentiation MiR-125b OSX Inhibit osteoblastic differentiation (Chen et al, 2014) mTOR, mammalian target of rapamycin; SMAD1, SMAD Family Member 1; TGIF, transforming growth factor-β induced factor; BMP7, Bone morphogenetic protein 7; GSK3β, Glycogen synthase kinase-3β; c-Myc, MYC proto-oncogene; HMGA1, high mobility group A; PPARγ, peroxisome proliferator activated receptor γ; Spry, Sprouty; OSX, osterix; HDAC 5, histone deacetylase 5; HOXA2, Homeobox A 2; RUNX2, Runt-related transcription factor 2; DKK1, Dickkopf 1; DKK1, Dickkopf 2; Krm2, Kremen2; sFRP2, secreted frizzled-related protein 2; SOST, sclerostin; STAT1, signal transducer and activator of transcription 1; BMPRII, bone morphogenetic protein receptor II; DLX, distal-less homeobox gene.…”

Section: Smad5mentioning

confidence: 99%

“…The functions of numerous unknown miRNAs require further exploration by researchers. For known miRNAs, the FIGURE 7 | Schematic drawing of miRNAs implicated in osteoblast differentiation (Li et al, 2009(Li et al, , 2012(Li et al, , 2015Hu et al, 2011;Wu et al, 2012;Zhang et al, 2012;Grunhagen and Ott, 2013;Liu et al, 2013Liu et al, , 2020Wang et al, 2013;Kato et al, 2014;Kureel et al, 2014;Li E. et al, 2014;Qi and Zhang, 2014;Sun et al, 2014Sun et al, , 2016Yang et al, 2014Yang et al, , 2017Xiao et al, 2016;Zheng et al, 2017;Xie and Cao, 2019;Krzeszinski et al, 2020;Yan et al, 2020). regulatory mechanism, selection of downstream target mRNAs and association with related diseases also requires further study.…”

Section: Other Mirnasmentioning

confidence: 99%

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The Roles of Epigenetics Regulation in Bone Metabolism and Osteoporosis

Yang

et al. 2021

Front. Cell Dev. Biol.

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Osteoporosis is a metabolic disease characterized by decreased bone mineral density and the destruction of bone microstructure, which can lead to increased bone fragility and risk of fracture. In recent years, with the deepening of the research on the pathological mechanism of osteoporosis, the research on epigenetics has made significant progress. Epigenetics refers to changes in gene expression levels that are not caused by changes in gene sequences, mainly including DNA methylation, histone modification, and non-coding RNAs (lncRNA, microRNA, and circRNA). Epigenetics play mainly a post-transcriptional regulatory role and have important functions in the biological signal regulatory network. Studies have shown that epigenetic mechanisms are closely related to osteogenic differentiation, osteogenesis, bone remodeling and other bone metabolism-related processes. Abnormal epigenetic regulation can lead to a series of bone metabolism-related diseases, such as osteoporosis. Considering the important role of epigenetic mechanisms in the regulation of bone metabolism, we mainly review the research progress on epigenetic mechanisms (DNA methylation, histone modification, and non-coding RNAs) in the osteogenic differentiation and the pathogenesis of osteoporosis to provide a new direction for the treatment of bone metabolism-related diseases.

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Section: Other Mirnasmentioning

confidence: 61%

Section: Smad5mentioning

confidence: 99%

Section: Other Mirnasmentioning

confidence: 99%

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The Roles of Epigenetics Regulation in Bone Metabolism and Osteoporosis

Yang

et al. 2021

Front. Cell Dev. Biol.

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“…Expanding this study to include more DPSC isolates with variable expression of these pluripotency biomarkers will provide substantial information that could validate the findings of the current study. In addition, a more comprehensive evaluation of other potential biomarkers, such as non-coding microRNA, may provide more specific and targeted methods for bioengineering and biotechnology applications utilizing DPSC [40][41][42].…”

Section: Discussionmentioning

confidence: 99%

Characterization of Dental Pulp Stem Cell Responses to Functional Biomaterials Including Mineralized Trioxide Aggregates

Bae

Kang

Lee

et al. 2021

JFB

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Introduction: Many studies in stem cell biology have demonstrated that dental pulp stem cells (DPSC) may be highly proliferative and capable of pluripotent differentiation into many different tissue types. Recent advances in stem cell research have outlined methods for directing in vitro or in vivo growth, viability, and proliferation, as well as differentiation of DPSC—although much remains to be discovered. Based upon this information, the primary objective of this study was to understand the functional biomaterials needed to more effectively direct DPSC viability, growth, and proliferation. Methods: Using an approved protocol, previously collected and isolated samples of DPSC from an existing repository were used. Previously established stem cell biomarkers (Sox-2, Oct-4, NANOG) from each isolate were correlated with their proliferation rates or doubling times to categorize them into rapid, intermediate, or slow-dividing multipotent DPSC. Growth factors and other functional dental biomaterials were subsequently tested to evaluate DPSC responses in proliferation, viability, and morphology. Results: Differential responses were observed among DPSC isolates to growth factors, including vascular endothelial growth factor (VEGF) and bone morphogenic protein (BMP-2), and functional biomaterials such as mineralized trioxide aggregates (MTA). The responsiveness of DPSC isolates did not correlate with any single factor but rather with a combination of proliferation rate and biomarker expression. Conclusions: These data strongly suggest that some, but not all, DPSC isolates are capable of a robust and significant in vitro response to differentiation stimuli, although this response is not universal. Although some biomarkers and phenotypes that distinguish and characterize these DPSC isolates may facilitate the ability to predict growth, viability, and differentiation potential, more research is needed to determine the other intrinsic and extrinsic factors that may contribute to and modulate these DPSC responses to these functional biomaterials for biotechnology and bioengineering applications.

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“…It has been found that lncRNA can regulate the expression of miRNA and play a role in tumorigenesis [15].…”

Section: Introductionmentioning

confidence: 99%

Lnc-RNA CASC15 promotes HCC progression via modulation of Six1 targeted by miR-2355-5p

Han

Sui

Tao

et al. 2020

Preprint

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Background: Long-chain non-coding RNA (LncRNA) plays a key role in the biological processes of tumors. LncRNA CASC15 has been shown to be involved in the development of a variety of tumors. The study aimed to elucidate the mechanism of lncRNA CASC15 in the progression of hepatocellular carcinomas (HCC). Methods: qRT-PCR was used to detect the expression levels of CASC15, miR-2355-5p and Six1 mRNA in HCC tissues and cells. Six1 protein expression levels were detected by Western Blot. CCK-8 experiment, colony formation experiment, Edu staining and Transwell experiment analysis were used to analyze the effects of CASC15, miR-2355-5p and Six1 on cell proliferation, cell invasion and migration. The relationship between CASC15, miR-2355-5p and Six1 was analyzed using bioinformatics analysis and Luciferase. Result: CASC15 was raised in HCC tissues and HCC cells. Down-regulation of CASC15 inhibited the growth, migration, invasion and tumor growth of HCC cells. The expression level of miR-2355-5p was reduced in HCC tissues. In addition, miR-2355-5p inhibitor induced the growth, migration and invasion of HCC cells. MiR-2355-5p was predicted to be a downstream target of CASC15. The expression level of miR-2355-5p was negatively correlated with CASC15 in HCC tumor tissues. Six1 was predicted to be a downstream target of miR-30a-5p. In vitro and in vivo results showed that CASC15/miR-2355-5p can regulate Six1.Conclusion: LncCASC15 regulated the proliferation and invasion of Six1 by binding with miR-2355-5p in HCC, suggesting that CASC15 may be a potential target for HCC.

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Differential expression of lncRNA/miRNA/mRNA and their related functional networks during the osteogenic/odontogenic differentiation of dental pulp stem cells

Cited by 47 publications

References 41 publications

The Roles of Epigenetics Regulation in Bone Metabolism and Osteoporosis

The Roles of Epigenetics Regulation in Bone Metabolism and Osteoporosis

Characterization of Dental Pulp Stem Cell Responses to Functional Biomaterials Including Mineralized Trioxide Aggregates

Lnc-RNA CASC15 promotes HCC progression via modulation of Six1 targeted by miR-2355-5p

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