“…Out of them 155 animals reacted positively with ELISA, while 809 animals reacted negatively in the farm with a prevalence rate 16.1%. The prevalence of paratuberculosis was in parallel to that recorded by (Abraham et al, 2014) who reported that the prevalence of paratuberculosis among dairy cattle was (16%). In another side, the obtained results are different from the result obtained by (Fawzy et al, 2013), who found that, the highest prevalence rate of the disease among the examined farms, that may be attributed to size of sample collected and method of diagnosis and the prevalence rate was 46.6%.…”