Comparison of two leukapheresis programs for computerized collection of blood progenitor cells on a new cell separator

Abstract

The LP-MNC program shows a significantly better CD34+ cell collection efficiency than the LP-PBSC-Lym program. However, collections with the LP-MNC program result in PBPC components with a lower MNC and CD34+ cell concentrations and a higher apheresis-related loss of patient's platelets.

Comparison of CD34+ cell collection on the CS‐3000+ and Amicus blood cell separators

“…The first major result of this comparison is a higher CD34+eff on the Amicus than on the CS3000+ separator, confirming, in two groups of patients, the first results obtained with one group of patients 3 . To better explore this point, we calculated the CD34+eff with respect to the initial CD34+ cell concentration, which could influence the leukapheresis efficiency 8 . Usually, the arbitrary thresholds of initial values of CD34+ cell concentrations of 36 or 40 per µL were used 6,8 .…”

“…To better explore this point, we calculated the CD34+eff with respect to the initial CD34+ cell concentration, which could influence the leukapheresis efficiency 8 . Usually, the arbitrary thresholds of initial values of CD34+ cell concentrations of 36 or 40 per µL were used 6,8 . To improve this approach, we studied four populations divided arbitrarily on the basis of discrete distribution CD34+ concentration per CD34+ yield: 5 to 50 CD34+ per µL (poorer mobilizers); 51 to 200 CD34+ per µL (good mobilizers); 201 to 500 CD34+ per µL (very good mobilizers); and more than 501 CD34 per µL (excellent mobilizers).…”

Combination of Cobe AutoPBSC and Gambro Elutra as a platform for monocyte enrichment in dendritic cell (DC) therapy: Clinical study

“…Despite the advantages of a high yield associated with LVL, several possible side effects associated with LVL have been reported, including increased incidence of citrate related reactions [17] and LVL associated platelets loss [18]. In our study group, no unusual incidence of citrate effects during LVL was observed, which probably resulted from the continuous infusion of Ca 21 .…”

Safe And Efficient Collection of Cytokine-Mobilized Peripheral Blood Cells From Cynomolgus Monkeys (Macaca fascicularis) with Human Newborn-Equivalent Body Weights

“…Our modified protocol significantly improved mononuclear and CD34 + cell harvest compared to the manufacturer's protocol. Under our modified procedure, we routinely collected 5 × 10 6 CD34 + cells per kg, which is equivalent to numbers in human trials published in the literature [13,25,28,31]. Thus, with our modified protocol, it is possible to collect sufficient numbers of CD34 + stem cells for various applications including transplantation experiments in monkeys.…”

“…of leukapheresis for adults [10,13,25,28] and children [1,8,15,31], only one very limited trial has been conducted on a human newborn baby [32]. To our knowledge, this paper is the first systematic documentation of leukapheresis for small primates.…”