Safe And Efficient Collection of Cytokine-Mobilized Peripheral Blood Cells From Cynomolgus Monkeys (Macaca fascicularis) with Human Newborn-Equivalent Body Weights

Ageyama, Naohide; Hanazono, Yutaka; Shibata, Hiroaki; Ono, Fumiko; Ogawa, Hiromi; Nagashima, Takahiro; Ueda, Yasuji; Yoshikawa, Yasuhiro; Hasegawa, Mamoru; Ozawa, Keiya; Terao, Keiji

doi:10.1538/expanim.54.421

Cited by 4 publications

(2 citation statements)

References 33 publications

(36 reference statements)

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“…In this report, we detail the safe apheresis of mobilized and nonmobilized cynomolgus macaques weighing 3 to 10 kg. While apheresis of macaques has been previously reported, 8,[17][18][19] this is the first report describing apheresis of cynomolgus macaques and of macaques weighing as low as 3 kg using Spectra Optia. Indeed, these previous descriptions of macaque apheresis were insufficient to facilitate successful leukapheresis of small cynomolgus macaques.…”

Section: Discussionmentioning

confidence: 99%

Terumo spectra optia leukapheresis of cynomolgus macaques for hematopoietic stem cell and T cell collection

Greene

Swanson

et al. 2020

J of Clinical Apheresis

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Macaques are physiologically relevant animal models of human immunology and infectious disease that have provided key insights and advanced clinical treatment in transplantation, vaccinology, and HIV/AIDS. However, the small size of macaques is a stumbling block for studies requiring large numbers of cells, such as hematopoietic stem cells (HSCs) for transplantation, antigenspecific lymphocytes for in-depth immunological analysis, and latentlyinfected CD4+ T-cells for HIV cure studies. Here, we provide a detailed protocol for collection of large numbers of HSCs and T-cells from cynomolgus macaques as small as 3 kg using the Terumo Spectra Optia apheresis system, yielding an average of 5.0 × 10 9 total nucleated cells from mobilized animals and 1.2 × 10 9 total nucleated cells from nonmobilized animals per procedure. This report provides sufficient detail to adapt this apheresis technique at other institutions, which will facilitate more efficient and detailed analysis of HSCs and their progeny blood cells.

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Section: Discussionmentioning

confidence: 99%

Terumo spectra optia leukapheresis of cynomolgus macaques for hematopoietic stem cell and T cell collection

Greene

Swanson

et al. 2020

J of Clinical Apheresis

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“…The technique is based on the mobilisation of bone marrow cells into the peripheral blood by the administration of exogenous granulocyte colony stimulating factor (G-CSF) [ 8 ]. Many studies have described the use of apheresis to collect G-CSF stimulated PBMC in man and apheresis has been described in a number of animal species including dogs [ 9 , 10 ], monkeys [ 11 ] and swine [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Peripheral mononuclear blood cell apheresis in a preclinical ovine model

et al. 2018

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BackgroundRecent research has demonstrated that circulating peripheral blood mononuclear fractions (PBMC) containing haematopoietic stem (HSC)/progenitor cells have the potential to play a crucial role in regenerative medicine strategies. Work in our laboratory has shown that a peripheral blood mononuclear cell fraction (PBMC) enhances cartilage repair in an osteochondral defect model in sheep and has a significant effect on cells in the joint niche. In order to obtain PBMC rich blood containing HSCs for further studies, we have performed, for the first time, apheresis on adult sheep.ResultsSubcutaneous granulocyte-colony stimulating factor (G-CSF) was used to mobilise white blood cells and continual flow apheresis was performed on 8 sheep under general anaesthetic. There were no observable side effects, although a marked tendency for blood clotting during the procedure was noted. The administration of G-CSF for 3 days increased the white blood cell (WBC) count in the peripheral blood from to 6.7 ± 2.1 × 106/ml to 16.1 ± 5.0 × 106/ml. Following apheresis, the WBC numbers in the apheretic product increased to 38.5 ± 27.6 × 106/ml, comprised of a significant increase in neutrophils and PBMC (from 5.25 ± 1.8 × 106/ml following G-CSF stimulation to 27.5 5 ± 27.6 × 106/ml). There was a mean of 2.1% CD34 + ve cells and 95.5% CD45 + ve cells in the apheretic product.ConclusionsThis study describes the administration of G-CSF and subsequent apheresis in adult sheep. The technique is safe when performed as described with no observable side effects. The technique permits collection of an increased WBC fraction containing neutrophils and PBMC in adult sheep. This apheretic product contains CD34 + ve cells, representing an HSC/progenitor population for use in in vivo and in vitro experiments.

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History of Hematopoietic Stem Cell Transplantation

Gatti

Storb

2014

Primary Immunodeficiency Disorders

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Safe And Efficient Collection of Cytokine-Mobilized Peripheral Blood Cells From Cynomolgus Monkeys (Macaca fascicularis) with Human Newborn-Equivalent Body Weights

Cited by 4 publications

References 33 publications

Terumo spectra optia leukapheresis of cynomolgus macaques for hematopoietic stem cell and T cell collection

Terumo spectra optia leukapheresis of cynomolgus macaques for hematopoietic stem cell and T cell collection

Peripheral mononuclear blood cell apheresis in a preclinical ovine model

History of Hematopoietic Stem Cell Transplantation

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