Comparison of three methods for thermostable C-phycocyanin separation and purification

Antecka, Anna; Klepacz-Smółka, Anna; Szeląg, Rafał; Pietrzyk, Damian; Ledakowicz, S.

doi:10.1016/j.cep.2021.108563

Cited by 17 publications

(11 citation statements)

References 38 publications

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“…The process with air flow of 2.4 L•h -1 resulted in partitioning coefficients of about 39, which is three times higher than previously reported for the same system (Antecka et al, 2022). With regard to pH, for most of the systems studied, the value of the partitioning coefficient was around 15, with an increase observed for pH 10 and a decrease for pH 5.…”

Section: Foam Fractionationmentioning

confidence: 47%

“…The experimental set-up for foam fractionation was previously described in Antecka et al (Antecka et al, 2022). In this work the influence of air flow rate, temperature and pH value, as well as the addition of surfactants were thoroughly investigated.…”

Section: Foam Fractionationmentioning

confidence: 99%

“…The experiments were performed in the specially designed extraction vessels similar to those presented by Prinz et al (Prinz et al, 2012). First, the stock solutions of PEGs and salts as well as the crude extract of PBPs were transferred into the mixing parts of the vessels and complemented with deionized water like in Antecka et al (Antecka et al, 2022). Then the vessels with all components were put on a magnetic stirrer and stirred for an hour at 300 rpm to achieve phase equilibrium between the phases.…”

Section: Aqueous Two-phase Extractionmentioning

confidence: 99%

“…It is an attractive method of biomolecule concentration, due to its mild conditions, relatively low cost, and scale-up potential (Iqbal et al, 2016). Both methods have already been applied to C-PC (Antecka et al, 2022), but not all parameters affecting the processes have been studied. Moreover, despite the advantages described, the results obtained were not on par with chromatographic methods.…”

Section: Introductionmentioning

confidence: 99%

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Separation and purification of phycobiliproteins from Thermosynechococcus PCC 6715 by foam fractionation and aqueous twophase extraction

Antecka,

Szeląg,

Ledakowicz

2024

Chemical and Process Engineering: New Frontiers

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The use of foam fractionation followed by aqueous two-phase extraction has emerged as a potential alternative to traditional liquid chromatography, hitherto irreplaceable in the purification of phycobiliproteins. The crude extracts of C-phycocyanin and allophycocyanin were obtained after Thermosynechococcus PCC 6715 biomass disintegration. The FF process with air flow of 2.4 L·h -1 resulted in purification factors up to 1.47 and partitioning coefficients of about 39, and did not require the addition of surfactants. A temperature of 35˚C allowed for the highest partitioning coefficient of 67.6 and yield of 76%; however, the purity of C-PC in condensate at this temperature was lower than at 25˚C. ATPE was tested in 20 different systems consisting of polyethylene glycol and phosphate or citrate salts, of which PEG1500-citrate gave the highest purification factor value of 2.31. Conversely, a partitioning coefficient of 2416 and 1094 were obtained for the PEG1500-phosphate and PEG3000-phosphate systems, respectively. Interestingly, the use of FF condensate in subsequent ATPE step resulted, for the first time, in the separation of the polymer phase into two fractions, one contained C-phycocyanin and the other allophycocyanin. It can be concluded that the use of a two-step system of FF and ATPE is a viable way to separate phycobiliproteins.

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Section: Foam Fractionationmentioning

confidence: 47%

Section: Foam Fractionationmentioning

confidence: 99%

Section: Aqueous Two-phase Extractionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Separation and purification of phycobiliproteins from Thermosynechococcus PCC 6715 by foam fractionation and aqueous twophase extraction

Antecka,

Szeląg,

Ledakowicz

2024

Chemical and Process Engineering: New Frontiers

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“…The freezing and thawing extraction process was accomplished according to Antecka et al [20]. After pre-soaking in distilled water, the predetermined weights of defatted Arthrospira platensis were dispersed at a ratio of 1:20 (S/L).…”

Section: Freezing and Thawingmentioning

confidence: 99%

Effect of Different Extraction Methods on Physicochemical Characteristics and Antioxidant Activity of C-Phycocyanin from Dry Biomass of Arthrospira platensis

Chen

Xiong

et al. 2022

Foods

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The effect of four different extraction methods on physicochemical characteristics and functionalities of chloro-phycocyanin (CP) was investigated. Swelling (S-CP), freezing and thawing (4FT-CP), ultrasonication with freezing and thawing (4FT+U-CP), and the high-pressure cell disruption (HPCD-CP) process affected CP differently, thus resulting in different levels of solubility, DPPH scavenging activity, ABTS scavenging activity, and reducing power. Among the four CPs, HPCD-CP had the highest CP content (15.3%), purity (1.66 ± 0.16), and ∆E value but the lowest ∆b value. The ζ potential of HPCD-CP (−38.8 mV) was the highest, but the average particle size of 4FT+U-CP (719.1 nm) was the highest. UV-Vis absorption spectra and fluorescence spectra illustrated that high-pressure cell disruption-assisted extraction had more profound impacts on the microenvironment of tetrapyrrole chromophores, the environment of aromatic amino acids, and the phycocyanobilin of CP. Furthermore, HPCD-CP and 4FT-CP showed higher solubility and antioxidant activities than S-CP, especially 4FT+U-CP. The results obtained in this study demonstrate that HPCD technology could obtain a food-grade C-phycocyanin product with higher CP concentration, purity, solubility, and antioxidant activity.

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Storage, Processing, and Stability of Phycobilins

Tariq,

Ali,

Basharat

et al. 2024

Pharmaceutical and Nutraceutical Potential of Cyanobacteria

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Comparison of three methods for thermostable C-phycocyanin separation and purification

Cited by 17 publications

References 38 publications

Separation and purification of phycobiliproteins from Thermosynechococcus PCC 6715 by foam fractionation and aqueous twophase extraction

Separation and purification of phycobiliproteins from Thermosynechococcus PCC 6715 by foam fractionation and aqueous twophase extraction

Effect of Different Extraction Methods on Physicochemical Characteristics and Antioxidant Activity of C-Phycocyanin from Dry Biomass of Arthrospira platensis

Storage, Processing, and Stability of Phycobilins

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