Separation and purification of phycobiliproteins from Thermosynechococcus PCC 6715 by foam fractionation and aqueous twophase extraction
Anna Antecka,
Rafał Szeląg,
Stanisław Ledakowicz
Abstract:The use of foam fractionation followed by aqueous two-phase extraction has emerged as a potential alternative to traditional liquid chromatography, hitherto irreplaceable in the purification of phycobiliproteins. The crude extracts of C-phycocyanin and allophycocyanin were obtained after Thermosynechococcus PCC 6715 biomass disintegration. The FF process with air flow of 2.4 L·h -1 resulted in purification factors up to 1.47 and partitioning coefficients of about 39, and did not require the addition of surfact… Show more
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