Comparison of the Troponin T and Troponin I ELISA Tests, as Measured by Microplate Immunoassay Techniques, in Diagnosing Acute Myocardial Infarction

Abstract

Summary:We describe an improved procedure using a standard microplate immunoassay reader to measure the concentration of troponin T in human serum. We also describe an immunoassay for troponin I in serum. Only 160 μΐ of serum are needed for a single analysis of each troponin. For comparison, creatine kinase MB mass analysis in serum was performed with a commercial luminometric method. From 95 apparently healthy people the following values were obtained: creatine kinase MB mass 2.6 ±1.2 μg/l, troponin Τ 0.027 ± 0.025 μg/l and troponin I 0.03 ± 0.031We compared the results of troponin Τ and troponin I methods with each other, as well as with those of creatine kinase MB mass measured in 48 patients with verified acute myocardial infarction and in 60 control patients with non-cardiac chest pain. The correlation between troponin Τ and troponin I values was 0.91 for the total material and 0.94 for 48 patients with acute myocardial infarction. Troponin I showed better earlier sensitivity than troponin Τ (ρ = 0.043). In nine patients in the control group, creatine kinase MB mass exceeded the reference limit of 5.0 μg/l, while in two patients the cut-off limit of 10.0 μ §/1 was also surpassed, pointing to non-specificity.In the group of infarct patients, the highest serum creatinine value was 193 μηιοΐ/ΐ, whereas in the control group it was 406 μιηοΙ/1. The sera of patients with impaired renal function without any cardiac failure showed no increase in troponin Τ and troponin I values.In conclusion, serum creatine kinase MB mass and troponin I seem to confirm an acute myocardial infarction more rapidly than does troponin T; troponin I has the highest cardiac specificity.

Sinapic Acid Attenuates Cardiovascular Disorders in Rats by Modulating Reactive Oxygen Species and Angiotensin Receptor Expression

“…The Cloud-Clone Corp. Rat Troponin I ELISA kit (Cloud-Clone Crop., Houston, USA) was employed to conduct a quantitative sandwich immunoassay for determining the level of troponin I in the serum in keeping with the approach suggested by Penttilä et al [ 30 ]. Meanwhile, the level of creatine kinase-MB enzyme in the serum (CK-MB) was determined with the assay kit supplied by Bioassay Systems (Hayward, CA, USA) and based on the method by Bishop et al [ 31 ].…”

“…Oxidative Medicine and Cellular Longevity serum in keeping with the approach suggested by Penttilä et al [30]. Meanwhile, the level of creatine kinase-MB enzyme in the serum (CK-MB) was determined with the assay kit supplied by Bioassay Systems (Hayward, CA, USA) and based on the method by Bishop et al [31].…”

Proanthocyanidin alleviates doxorubicin‐induced cardiac injury by inhibiting NF‐kB pathway and modulating oxidative stress, cell cycle, and fibrogenesis

“…The CK activity was analyzed according to Szasz et al, [ 19 ] whereas serum CK‐MB activity was analyzed according to Würzburg et al [ 20 ] Serum IL‐2, IL‐6, and TNF‐α were measured using ELISA kits from R&D, following the manufacturer's instructions. Serum troponin I levels were also determined using an ELISA kit (Spectrum Diagnostics), as described by Penttilä et al [ 21 ]…”

“…The CK activity was analyzed according to Szasz et al, [19] whereas serum CK-MB activity was analyzed according to Würzburg et al [20] Serum IL-2, IL-6, and TNF-α were measured using ELISA kits from R&D, following the manufacturer's instructions. Serum troponin I levels were also determined using an ELISA kit (Spectrum Diagnostics), as described by Penttilä et al [21] 2.5 | Determination of oxidative stress state Catalase (CAT) was analyzed according to Aebi, [22] SOD according to Nishikimi et al, [23] GST according to Mohandas et al, [24] and GSH according to Beutler et al [25] Lipid peroxidation (LPO) was analyzed by calculating the amount of malondialdehyde (MDA) according to Uchiyama et al [26] The rate of protein oxidation was analyzed by determining total protein carbonyl (C═O) levels according to Carty et al [27] using the molar absorption coefficient of 22,000 M −1 cm −1 .…”

Studies towards hcTnI Immunodetection Using Electrochemical Approaches Based on Magnetic Microbeads

“…In this context, different immunological methodologies have been developed to detect hcTnI in human samples. Electrochemiluminiscent immunoassays (ECLIA) [ 14 , 15 , 16 ], Fluoroimmunoassays [ 16 , 17 , 18 , 19 , 20 ], and enzyme-linked immunosorbent assay (ELISA) [ 21 , 22 , 23 , 24 ] have been widely used for AMI diagnosis. However, these techniques require expensive laboratory equipment, well-trained personnel and are time-consuming techniques, especially taking into account that a timely and reliable diagnosis is required for appropriate patient treatment.…”